Navigation Links
Escherichia coli K12

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.514 04/2002 Microorganism Escherichia coli K12 Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pUC 19) Growth medium LB medium Washing solution Sterile, ice-cold water; (10% glycerol) Electroporation solution Sterile, ice-cold water; (10% glycerol) Outgrowth medium SOC medium (without antibiotics) Cuvette 1 mm gap width Reference Eppendorf AG Application Hotline D-22331 Hamburg
Phone +49 180 3 666 789 Fax +49 40 53990 125 e-mail: application-hotline@eppendorf.de
Adapted from: High Efficiency Transformation by Electroporation Short Protocols in Molecular Biology
Second Edition Green Publishing Association and John Wiley & Sons, New York 1-22 1-23 Making electrocompetent cells:

1. Inoculate 500 ml LB medium with 2.5 ml of a fresh overnight culture of E. coli DH10B. Grow at 37 C with shaking to an O.D.600 of 0.5 to 0.6. 2. Chill cells on ic
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Escherichia coli C600
2. Escherichia coli DH10B
3. Escherichia coli DH5
4. Escherichia coli C600
5. Escherichia coli K12
6. Escherichia coli DH10B
7. Escherichia coli DH5
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Escherichia coli

(Date:1/14/2014)... As pet owners drew up their lists of New Year,s resolutions ... care of my furry companion." Nowadays there are pet salons, dog ... to take the little canine or feline darlings along wherever mommy ... some pricey toys at the pet store. But anyone who has ...
(Date:1/14/2014)... AUSTIN, Texas , Jan. 14, 2014 ... analyze and research medicinal plants and therapeutic derivatives ... industry, health professionals, and researchers about the challenges ... (Logo: http://photos.prnewswire.com/prnh/20100430/DC95601LOGO ) The ...
(Date:1/14/2014)... iLab Solutions, the leader in core facility management software, today ... In this role, Michelle will work with current and potential ... development of iLab products. Her main goal will be to ... scientific community by offering the most advanced, user-friendly, and high-impact ...
(Date:1/14/2014)... 2014  RXi Pharmaceuticals Corporation (OTCQX: RXII), a biotechnology ... therapies addressing major unmet medical needs using RNA-targeted ... of Allowance from the United States Patent and ... (sd-rxRNA®), for the treatment of fibrosis. The patent ...
Breaking Biology Technology:New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3
... the College,s history, BRONX, N.Y., May 13 ... University today announced that it has received a ... largest in the College,s 53-year history,-- from Ruth ... the Gottesmans is,available., (Photo: http://www.newscom.com/cgi-bin/prnh/20080513/NYTU059-a ), ...
... Milestone, NEW YORK, May 13 NeoStem, Inc. ... processing and long-term,storage of adult stem cells for future ... for a new stem cell collection center in Coral,Gables, ... MBA, NeoStem,s Chairman and Chief Executive Officer,commented: "Securing the ...
... Pacific Time, SAN DIEGO, May 13 SGX ... for the first quarter,of 2008. For the three months ... $17.0 million and net income of $3.7 million. The ... that was,attributable to the one-time recognition of the remaining ...
Cached Biology Technology:Albert Einstein College of Medicine Receives $25-Million Gift to Support Stem Cell and Epigenomic Research and Clinical Skills Training 2Albert Einstein College of Medicine Receives $25-Million Gift to Support Stem Cell and Epigenomic Research and Clinical Skills Training 3Albert Einstein College of Medicine Receives $25-Million Gift to Support Stem Cell and Epigenomic Research and Clinical Skills Training 4Albert Einstein College of Medicine Receives $25-Million Gift to Support Stem Cell and Epigenomic Research and Clinical Skills Training 5NeoStem to Expand Stem Cell Collection Center Network to Florida 2NeoStem to Expand Stem Cell Collection Center Network to Florida 3SGX Pharmaceuticals Announces First Quarter Financial Results and Gives Pipeline Update 2SGX Pharmaceuticals Announces First Quarter Financial Results and Gives Pipeline Update 3SGX Pharmaceuticals Announces First Quarter Financial Results and Gives Pipeline Update 4SGX Pharmaceuticals Announces First Quarter Financial Results and Gives Pipeline Update 5SGX Pharmaceuticals Announces First Quarter Financial Results and Gives Pipeline Update 6SGX Pharmaceuticals Announces First Quarter Financial Results and Gives Pipeline Update 7SGX Pharmaceuticals Announces First Quarter Financial Results and Gives Pipeline Update 8
(Date:4/17/2014)... Clemson professor Rajendra Singh Thursday as a "Champion ... expand solar deployment in the residential, commercial and ... Professor of Electrical and Computer Engineering and director ... a local hero leading the charge across the ... solar power and driving policy changes at the ...
(Date:4/17/2014)... devastate honeybees in Europe, Asia and the United States ... to be impacting native honeybee populations at this time, ... pests include including Nosema microsporidia and ... be resilient to these invasive pests, which suggests to ... Europe, Asia and the United States currently are not ...
(Date:4/17/2014)... France have hit on a novel method to ... correct and most effective treatment possible., Kidney stones ... and developing world. If left untreated, apart from ... failure and other complications. In many patients treated ... a more effective pathological approach to diagnosis and ...
Breaking Biology News(10 mins):White House honors Clemson professor as 'Champion of Change' for solar deployment 2East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3
... Manchester have successfully extracted protein from the bones of ... the identification of ancient fossils. Using an ultra-high ... near complete collagen sequence for the West Runton Elephant, ... in Norfolk in 1990. The remarkable 85 per cent ...
... new series of events at the New York Academy ... that may offer large cities sustainable alternatives to landfill ... "Trash Talk" series on Thursday, April 7, will examine ... treatment. Experts will explain how these technologies ...
... pollutants whose lack of solubility in water, or "hydrophobicity," has ... Aviv University research has discovered that this is indeed the ... University,s School of Chemistry at the Raymond and Beverly Sackler ... TAU,s Laboratory of Nanomedicine in the Department of Cell Research ...
Cached Biology News:West Runton Elephant helps unlock the past 2Mucus: Fighting the war against pollutants 2
...
... Sample Preparation for Membrane Proteins ,Contains ... membrane,protein extracts for 2-D gel analysis ... Extraction Kit , (contains sufficient ... a packed cell weight of 10 mg ...
Mouse monoclonal antibody raised against a partial recombinant AAAS. NCBI Entrez Gene ID = AAAS...
... Amplifluor UniPrimer-fluorescein is a hairpin primer ... pair (fluorescein and non-fluorescent quencher) and ... The UniPrimer is identical to the ... Detection System (S7901) and is provided ...
Biology Products: