Navigation Links
Escherichia coli DH5

nd transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 x g, 2-4 C). Resuspend pellet in 5 ml ice-cold water. Keep the cells cold during the entire procedure. 3. Wash twice with the original culture volume of ice-cold water. Centrifuge as above. Resuspend pellet by swirling in remaining liquid. 4a. If using the cells immediately, place suspension in a prechilled tube and centrifuge (10 minutes, 5,000 x g, 2-4 C). Resuspend the cells in ice-cold water to a final concentration of approximately 2 x 1011 cells/ml. Aliquote 40-300 l cells into prechilled centrifuge tubes. 4b. If freezing the cells for later use, add 40 ml of ice-cold 10% glycerol, mix and centrifuge for 10 minutes, at 5,000 x g and 2-4 C. Resuspend the cells in ice-cold 10% glycerol to a final concentration of approximately, 2 x 1011 cells/ml. Aliquote 40-300 l cells into prechilled centrifuge tubes. quick freeze on dry ice and store at 80 C.

Electroporation of cells:

  1. Add 1 l DNA (10 pg in water) to tubes containing 40 l electrocompetent cells. Homogenize by gently mixing with
    pipette several times.
  2. Transfer mixture to a prechilled cuvette. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O Voltage (V)
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Escherichia coli C600
2. Escherichia coli DH10B
3. Escherichia coli DH5
4. Escherichia coli K12
5. Escherichia coli C600
6. Escherichia coli K12
7. Escherichia coli DH10B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Escherichia coli

(Date:12/24/2014)... and NEW YORK , Dec. ... biopharmaceutical company advancing patient care in critical areas, announced the ... common stock, and warrants to purchase up to an aggregate ... $4.00 per share and $.01 per warrant.  The warrants have ...
(Date:12/24/2014)... Solutions , Inc. (NASDAQ: BLFS ), a leading ... storage and cryopreservation freeze media and ... or the "Company"), today announced that it will hold its ... "Annual Meeting"). Because the expected date for the ...
(Date:12/24/2014)... Missouri (PRWEB) December 23, 2014 GMO corn ... company, Syngenta, are in the process of being consolidated in ... is In Re: Syngenta AG MIR 162 Corn Litigation, MDL ... of Kansas. , Management of the Syngenta GMO corn multidistrict ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 ... designed to introduce cutting edge communication technology, provide ... platform upgrades. This webinar series demonstrates online communication ... to leverage web conferencing’s most innovative and powerful ...
Breaking Biology Technology:PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 2PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 3BioLife Solutions Sets Date for Annual Meeting of Stockholders 2Carey Danis & Lowe Reports on Syngenta GMO Corn Transfer Order 2SoundConnect Launches Leading Edge Business Communications Webinar Series 2SoundConnect Launches Leading Edge Business Communications Webinar Series 3
... , EATONTOWN, N.J. , Jan. 22 Osteotech, ... of biologic products for regenerative healing, announced today that its Board ... designed to discourage coercive or unfair takeover tactics and to provide ... the event that an unsolicited offer is made to acquire the ...
... Twenty leading US research institutions, patient advocates and biomedical industry ... 2009 Recovery and Reinvestment Act , WASHINGTON , ... anniversary of the Recovery and Reinvestment Act,s enactment on ... patient advocates and biomedical industry have released a detailed report ...
... ... QCam imaging driver for QImaging cameras. This interface provides support for Microsoft,s 64-bit ... faster analysis. , ... (PRWEB) January 20, 2010 -- QImaging, a leading developer and manufacturer of high-performance, ...
Cached Biology Technology:Osteotech, Inc. Adopts Stockholder Rights Plan 2Osteotech, Inc. Adopts Stockholder Rights Plan 3A Recovery Act Success Story: NIH Funding of Biomedical Innovation Is Creating Jobs, Fueling Industry Growth, and Helping Reduce Long-term Health Care Costs 2A Recovery Act Success Story: NIH Funding of Biomedical Innovation Is Creating Jobs, Fueling Industry Growth, and Helping Reduce Long-term Health Care Costs 3QImaging Introduces 64-Bit QCam Driver 2
(Date:1/22/2015)...  A man-made form of insulin delivered by nasal spray ... adults with mild cognitive impairment and Alzheimer,s disease dementia, ... Wake Forest Baptist Medical Center. The study,s ... impairment (MCI) or mild to moderate Alzheimer,s dementia (AD). ...
(Date:1/22/2015)... , Europe,s most prestigious innovation prize ... present a video retrospective of the most exciting and innovative nominations  ... and Sophie Wilson   A different theme ... Starting on 22 January 2015 on   http://www.epo.org/european-inventor   ...
(Date:12/24/2014)... 2014  Since its launch in December 2014, the 1U™ ... pain of trying to remember their usernames and passwords through ... their smartphones. To assist people who have struggled to remember ... created 1U and focuses on redefining identity, announced today that ...
Breaking Biology News(10 mins):Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 31U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3
... the genetic basis of bipolar disorder, a highly heritable ... depression. The research, published by Cell Press online February ... , identifies a previously unrecognized susceptibility factor for bipolar ... to systematically sort through all the DNA of many ...
... All night long, bats swoop over our landscape consuming ... view. Until recently, scientists have been unable to bring ... genetic techniques, researchers from the University of Bristol and Biodiversity ... environment supporting these elusive creatures. Working at three ...
... With the steep decline in populations of many animal species, scientists ... extinction like those that have occurred just five times during the ... three-quarters or more of all animal species go extinct. In ... Nature , researchers report on an assessment of where mammals ...
Cached Biology News:Scientists identify susceptibility factor for bipolar disorder 2Food forensics: DNA links habitat quality to bat diet 2Earth's sixth mass extinction: Is it almost here? 2Earth's sixth mass extinction: Is it almost here? 3Earth's sixth mass extinction: Is it almost here? 4
0.2ml Gradient Block MBSR. Robot compatible block ejection mechanism. Stackable. (96 well block - holds 1 plate 0.2ml x 96 well plate)...
A Highly Purified, Cost-Effective Taq DNA Polymerase...
... Synonym: Dinitro-5,5'-dithiodibenzoic acid , DTNB , ... 396.36 g/mol , *CAS-No.: 69-78-3 ... a product with a reactive grouping not present ... include a reagent which will react directly with ...
Cag-A (IgG) -Ab EIA Sample Size: 10 l...
Biology Products: