Navigation Links
Escherichia coli DH5

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.512 04/2002 Microorganism Escherichia coli DH5alpha Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pUC 19) Growth medium LB medium Washing solution Sterile, ice-cold water; (10% glycerol) Electroporation solution Sterile, ice-cold water; (10% glycerol) Outgrowth medium SOC medium (without antibiotics) Cuvette 1 mm gap width Reference Eppendorf AG Application Hotline D-22331 Hamburg
Phone +49 180 3 666 789 Fax +49 40 53990 125 e-mail: application-hotline@eppendorf.de
Adapted from: High Efficiency Transformation by Electroporation Short Protocols in Molecular Biology
Second Edition Green Publishing Association and John Wiley & Sons, New York 1-22 1-23 Making electrocompetent cells: 1. Inoculate 500 ml LB medium with 2.5 ml of a fresh overnight culture of E. coli DH5alpha. Grow at 37 C with shaking to an O.D.600 of 0.5 to 0.6. 2. Chill cells on ice for 15 minutes a
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Escherichia coli C600
2. Escherichia coli DH10B
3. Escherichia coli DH5
4. Escherichia coli K12
5. Escherichia coli C600
6. Escherichia coli K12
7. Escherichia coli DH10B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Escherichia coli

(Date:9/2/2014)... (PRWEB) September 02, 2014 Myriant ... the signage of a distribution agreement with Azelis ... now distributing Myriant’s bio-succinic acid in the Nordics, ... Ireland, targeting customers in the industrial and base ... powerhouse, Azelis offers a far-reaching chemical portfolio with ...
(Date:9/2/2014)... MONROVIA, Calif. , Sept. 2, 2014 /PRNewswire/ ... clinical-stage biopharmaceutical company developing engineered monoclonal antibodies for ... diseases, and cancer, today announced the appointment of ... clinical development and the appointment of Lloyd ... and general counsel. "Debra,s expertise ...
(Date:9/2/2014)... ON , Sept. 2, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: ... totalling $2.1 million. The orders consist of three large odour ... Canada . "These orders are consistent with ... said Peter Bruijns , President and CEO. "After a ... market, we are now beginning to see sales growth. Our ...
(Date:9/2/2014)... , September 2, 2014 ... BioData,s research and lab management services  BioData, ... service, is pleased to announce the appointment of ... most recently served as VP Marketing and Business Development ... science research and clinical applications. Previously, Culot ...
Breaking Biology Technology:Myriant and Azelis Sign European Distribution Agreement for Bio-Succinic Acid 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 2Xencor Appoints Debra Zack, M.D., Ph.D., Vice President, Clinical Development and Lloyd Rowland, Senior Vice President, Chief Compliance Officer and General Counsel 3Louis Culot Appointed as BioData's CEO 2
... 25 /Xinhua-PRNewswire-FirstCall/ -- 3SBio Inc.,(Nasdaq: SSRX ... researching,developing, manufacturing and marketing biopharmaceutical products,primarily in ... with the Chinese,State Food and Drug Administration ... formulation of EPIAO for the treatment of ...
... be Used for Advancement of Cancer Stem Cells and Other ... ... Inc., a privately,held biotechnology company that develops immunotherapeutics to treat,autoimmune disorders, ... in a Series D-2 financing. The round, led by,Nextech Venture, included ...
... COLLINS, Colo., Sept. 24 InVitria,( http://www.InVitria.com ... Annual "Optimizing Cell Culture Development" Conference on,September ... most recent,results optimizing cell culture media using ... ingredients.,( http://www.healthtech.com/ocb/overview.aspx?c=562 ), In a presentation ...
Cached Biology Technology:3SBio Inc. Files for SFDA Approval of High-dose (36,000 IU) EPIAO 23SBio Inc. Files for SFDA Approval of High-dose (36,000 IU) EPIAO 3MacroGenics Raises $25M in Series D-2 Financing 2MacroGenics Raises $25M in Series D-2 Financing 3MacroGenics Raises $25M in Series D-2 Financing 4InVitria Touts Product Advantages at Cambridge's Cell Culture Conference 2
(Date:9/2/2014)... Taiyuan, China, have substantially improved the health of people ... in costs associated with loss of life and disability ... Columbia Center for Children,s Environmental Health (CCCEH) at the ... the Center of Diseases Control and Prevention of Taiyuan ... , The study is the first to document the ...
(Date:9/2/2014)... DURHAM, N.C. -- When you accidentally touch a hot ... know the basic neural circuits involved in sensing and ... out the molecular players. , Duke researchers have made ... molecule involved in pain in worms, and have built ... Sept. 2 in Nature Communications , may help ...
(Date:9/1/2014)... Dietary quality in the U.S. has improved steadily in ... intakebut overall dietary quality remains poor and disparities continue ... a new study from Harvard School of Public Health ... to date that the extensive efforts by many groups ... some payoff, but it also indicates that these efforts ...
Breaking Biology News(10 mins):Clean air halves health costs in Chinese city 2Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3Quality of US diet shows modest improvement, but overall remains poor 2Quality of US diet shows modest improvement, but overall remains poor 3
... what you know often comes at the price of learning ... easy for many, but not for those with conditions such ... simple routines. Using brain scans in monkeys, Duke ... monkeys will switch from exploiting a known resource to exploring ...
... Taylor Chair of Chemistry Barry Dellinger was recently awarded a ... Health Sciences, or NIEHS, to support a Superfund Research Center ... health. It is the only center ever awarded by this ... school of public health. "This grant is the result of ...
... semi-decayed vegetation matter, has been used by commercial growers ... century. Peat is added to potting soil to help ... for peat grew, acres of peat bogs were being ... of extracting peat from wetlands are mounting. And as ...
Cached Biology News:Monkey brains signal the desire to explore 2LSU receives $3.6 million to fund center studying hazardous waste cleanup and health impacts 2For peat's sake: Alternative growing media 2
... sealed isolation chamber provides a safe, ... sterile pharmaceuticals, in accord with USP-797 ... 100 (ISO 5) cleanliness conditions in ... IV rod and tilt-up front panel ...
... Protein determined by Biuret. Physical ... (NH 4 ) 2 SO ... Note: Affinity purified • Physical ... Additives: MgCl 2 1 ...
Ideal for chemical processing that requires downward flow of exhaust fumes. Powderful 1200 CFM blower draws fumes into sub-surface bonded charcoal filter for safe removal of organic vapors (final HEP...
... using mass numbers as a trigger, precisely ... performing investigation and research of natural compounds. ... and preparative operation. Switching between modes is ... This support provides a quick solution for ...
Biology Products: