Navigation Links
Escherichia coli DH5

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.512 04/2002 Microorganism Escherichia coli DH5alpha Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pUC 19) Growth medium LB medium Washing solution Sterile, ice-cold water; (10% glycerol) Electroporation solution Sterile, ice-cold water; (10% glycerol) Outgrowth medium SOC medium (without antibiotics) Cuvette 1 mm gap width Reference Eppendorf AG Application Hotline D-22331 Hamburg
Phone +49 180 3 666 789 Fax +49 40 53990 125 e-mail: application-hotline@eppendorf.de
Adapted from: High Efficiency Transformation by Electroporation Short Protocols in Molecular Biology
Second Edition Green Publishing Association and John Wiley & Sons, New York 1-22 1-23 Making electrocompetent cells: 1. Inoculate 500 ml LB medium with 2.5 ml of a fresh overnight culture of E. coli DH5alpha. Grow at 37 C with shaking to an O.D.600 of 0.5 to 0.6. 2. Chill cells on ice for 15 minutes a
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Escherichia coli C600
2. Escherichia coli DH10B
3. Escherichia coli DH5
4. Escherichia coli K12
5. Escherichia coli C600
6. Escherichia coli K12
7. Escherichia coli DH10B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Escherichia coli

(Date:7/10/2014)... Terascala, the industry leader in ... a former vice president of product management for Flexera ... of marketing. Terascala’s software when combined with leading compute ... highest performance and most reliable solutions for processing big ... channel expansion and broaden its product portfolio. His leadership ...
(Date:7/10/2014)... 2014 Robert Harman, DVM, Founder and CEO ... proud to announce the relaunch of his highly informative blog, ... series called “ What are Stem Cells ?” Dr. ... straightforward foundation in the basics of stem cell therapy so ... right type of treatment when considering regenerative medicine. , A ...
(Date:7/10/2014)... EvoDerma ’s NOOME Anti-Aging Motion has been upgraded ... with a second treatment cup to help prevent and smooth ... is thinner on the edges for a softer contact with ... uneven surfaces on the face, neck and décolleté. Choose to ... areas such as the cheeks and neck, or opt for ...
(Date:7/10/2014)... POINT, Ind. , July 10, 2014 ... expanded its use of capnography for respiratory ... growing group of healthcare leaders in embracing ... measures how effectively patients are breathing and ... depression occurs. By measuring the amount of ...
Breaking Biology Technology:Terascala Expands Executive Team 2Terascala Expands Executive Team 3Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2
... MELBOURNE, Australia, Oct. 5 Cytopia Limited (ASX: CYT) ... BioSciences Inc. ("YM") have signed an exclusive and binding ... YM will acquire all of the issued shares and ... scheme(s) of arrangement ("Scheme(s)") and shareholders (and optionholders if ...
... Corporation (NYSE: BSX ) today announced its exclusive ... This new trial follows in the tradition of ... each exclusively sponsored by Boston Scientific and conducted under ... Professor of Medicine at the University of Rochester. , ...
... , UPPSALA, Sweden, October 5 ... SuperConvection(TM), - At One Sixth the Price of ... of PCR Expected to Rapidly Penetrate the Market , ... developed a,miniaturized PCR-instrument - AmpXpress(TM) - to meet demands for lower,instrument ...
Cached Biology Technology:Cytopia Merger with Toronto-based YM BioSciences Inc. 2Cytopia Merger with Toronto-based YM BioSciences Inc. 3Cytopia Merger with Toronto-based YM BioSciences Inc. 4Cytopia Merger with Toronto-based YM BioSciences Inc. 5Cytopia Merger with Toronto-based YM BioSciences Inc. 6Cytopia Merger with Toronto-based YM BioSciences Inc. 7Boston Scientific Announces Exclusive Sponsorship and First Enrollments of MADIT-RIT Clinical Trial 2Boston Scientific Announces Exclusive Sponsorship and First Enrollments of MADIT-RIT Clinical Trial 3AlphaHelix Presents AmpXpress(TM) at the BIOTECHNICA Show 2
(Date:7/10/2014)... , July 1, 2014 Smart ... way for convenience and improved security: NXT-ID, Inc. (OTCBB: NXTD), ... GOOG ), Apple Inc. (NASDAQ: AAPL ... Corporation (NYSE: MA) NXT-ID, Inc., (OTCQB: NXTD) a biometric ... to announce that the second series of 30 second spots ...
(Date:7/10/2014)... COMMACK, N.Y. , July 2, 2014 ... Inc. (ABD) to announce the appointment of the world-renowned ... its Board of Directors. Dr. Ginsburg, with ... two facilities: NY Presbyterian Hospital and Good Samaritan Regional ... Breathing Pacemaker since the late 1980,s and is considered ...
(Date:7/10/2014)... According to the new market research report, ... (Optical Prism, Pizeoelectric, Capacitive, and Adhesives), Application (Mobile Devices, ... and Geography - Global Trends & Forecasts to 2014 ... Market is projected to cross $14.35 Billion by 2020, ... to 2020. Browse more than 78 market ...
Breaking Biology News(10 mins):Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 4Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 5Avery Biomedical Devices, Inc. announces the appointment of Mark E Ginsburg, M.D. to its Board of Directors 2Fingerprint Sensors Market worth $14.35 Billion by 2020 2Fingerprint Sensors Market worth $14.35 Billion by 2020 3
... presents Research & Entrepreneurship Day on April 30th, 2010 ... Stevens campus. R&E Day is a showcase of ... and student based research programs. It is also ... success in engineering solutions for the betterment of mankind. ...
... Tech biologist proposes to use a physics strategy called resonant ... they will be sensitive to antibiotics. In medicine, resonance ... thumps your chest. In physics, resonance is a periodic force ... a natural system,s frequency. Sound waves are an example of ...
... , A new study identifies cattle feed as a ... San Joaquin Valley a moderately-populated agricultural region ... ingredients in smog) than many densely-populated cities. The report, ... exhausts in forming ozone, is in ACS, Environmental ...
Cached Biology News:Research & Entrepreneurship Day 2010: Engineering Innovation 2Physics strategy tested as solution for antibiotic resistance 2
... Microarrays designed for genome-wide microRNA ... flexible and powerful Paraflo microfluidic on-chip ... as part of our comprehensive microRNA ... Content The Soil Nematode microRNA ...
... to the maintenance of appropriate levels ... important and diverse as those involved ... stress response, antigen presentation, modulation of ... cell cycle regulation, transcription, and signalling ...
... modified digital scanner serves as the spot-picking ... five standard rack positions on the tray, ... well plates on the remaining two racks.,The ... spots by mouse-click on the scanned gel ...
... Cleanroom-grade vinyl curtains or ... powder-coated steel support frame to ... cleanroom that meets cleanliness to ... HEPA filter/fan units, lights, and ...
Biology Products: