Navigation Links
Escherichia coli DH5

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.512 04/2002 Microorganism Escherichia coli DH5alpha Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pUC 19) Growth medium LB medium Washing solution Sterile, ice-cold water; (10% glycerol) Electroporation solution Sterile, ice-cold water; (10% glycerol) Outgrowth medium SOC medium (without antibiotics) Cuvette 1 mm gap width Reference Eppendorf AG Application Hotline D-22331 Hamburg
Phone +49 180 3 666 789 Fax +49 40 53990 125 e-mail: application-hotline@eppendorf.de
Adapted from: High Efficiency Transformation by Electroporation Short Protocols in Molecular Biology
Second Edition Green Publishing Association and John Wiley & Sons, New York 1-22 1-23 Making electrocompetent cells: 1. Inoculate 500 ml LB medium with 2.5 ml of a fresh overnight culture of E. coli DH5alpha. Grow at 37 C with shaking to an O.D.600 of 0.5 to 0.6. 2. Chill cells on ice for 15 minutes a
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Escherichia coli C600
2. Escherichia coli DH10B
3. Escherichia coli K12
4. Escherichia coli C600
5. Escherichia coli K12
6. Escherichia coli DH10B
7. Escherichia coli DH5
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Escherichia coli

(Date:5/1/2015)... , May 1, 2015  Northwest Biotherapeutics (NASDAQ: ... DCVax® personalized immune therapies for solid tumor cancers, announced ... million second tranche of a $40 million investment from ... Woodford first invested $25 million in NW Bio on ... into an agreement with the Company for a further ...
(Date:5/1/2015)... 2015 A new report by  ... will reach $22.4bn in 2019. That revenue forecast and ... Market Prospects 2015-2025 ,  published in March ... to provide forecasts and qualitative analyses of the antithrombotic ... consultancy in London, UK . It ...
(Date:4/30/2015)... 2015 Spirax Sarco, the leader in ... to announce that for the first time in the ... of a Queen’s Award for Innovation – the UK’s ... manufacture of a unique flowmeter. , The Spirax Sarco ... traditional steam flow measurement. The device provides reliable, ...
(Date:4/30/2015)... Nitto Avecia, a leading biotechnology manufacturer and ... production capability to serve biotechnology pharmaceutical clients. A ... continues to drive Nitto Avecia to enhance synthesis ... , In Fall 2014, Nitto Avecia first ... its Milford, MA facility, which is recognized as ...
Breaking Biology Technology:NW Bio Closes On $28.5 Million Second Tranche Of Woodford $40 Million Financing 2NW Bio Closes On $28.5 Million Second Tranche Of Woodford $40 Million Financing 3Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 2Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 3Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 4Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 5Spirax Sarco Receives a Queen’s Award for Innovation 2Spirax Sarco Receives a Queen’s Award for Innovation 3Biotechnology Manufacturer Nitto Avecia Expands Capacity to Meet Client Demand 2
... of visible light and zinc oxide nanorods for destroying ... at the Asian Institute of Technology (AIT). Nanorods grown ... been found to be effective in killing both gram ... has immense possibilities for affordable and environmentally friendly water ...
... May 12, 2011 Stem Cell Assurance, Inc. (SCLZ.PK) ... for Registration of Securities on Form 10 pursuant to Section ... 10 includes audited financial statements as of December 31, 2009 ... this filing, effective July 11, 2011, the Company will become ...
... May 12, 2011 The Maryland Academy of Sciences ... recipients of its Annual Awards which recognize the accomplishments ... Dr. Ian Appelbaum, Associate Professor of Physics at the ... (OYS) award and Dr. Ganesh Sriram, Assistant Professor in ...
Cached Biology Technology:Exposing ZnO nanorods to visible light removes microbes 22011 Outstanding Young Scientist and Engineer Named; Baltimore City High School Students Awarded Glass Scholarships 2
(Date:4/21/2015)... , April 21, 2015 ... government policies are boosting access control systems market in ... According to a recently published report by TechSci Research ... ", the access control systems market in ... billion by 2020.The access control systems market in the ...
(Date:4/17/2015)... 2015 Increasing adoption ... advancement to drive biometric systems market in ... According to a recently released TechSci ... ", biometric systems market in Saudi ... at CAGR of over 22% through 2020. The ...
(Date:4/13/2015)... April 13, 2015 According ... "Global Biometrics Market Forecast & Opportunities, 2020", the global ... of around 14% till 2020. The biometrics market ... scale implementation and review of biometric management systems. ... new products with greater efficiency, are resulting in ...
Breaking Biology News(10 mins):Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2
... Drawing on records dating back to the journals of Henry ... plant families near Walden Pond have borne the effects of ... families hit hardest by global warming have included beloved species ... appears this week in the Proceedings of the National ...
... used to treat type 2 diabetes may be more likely ... at Washington University School of Medicine in St. Louis have ... drugs, sulfonylureas, help type 2 diabetics make more insulin, improving ... effects of sulfonylureas are lost after several years of use, ...
... carriers of avian influenza viruses from continent to continent ... has important implications for highly pathogenic avian influenza virus ... multi-pronged research effort to understand the role of migratory ... Asia and North America, scientists with the U.S. Geological ...
Cached Biology News:Effects of climate change vary greatly across plant families 2Moderate use averts failure of type 2 diabetes drugs in animal model 2Genetic evidence for avian influenza movement from Asia to North America via wild birds 2
37-1A9...
sPLA2-XII Human, Rabbit Polyconal Antibody Anti-Human sPLA2-XII Antibody was raised in rabbits against Human sPLA2-XII His-Tagged Fusion Protein Antigen: The recombinant human protein sPLA-XII...
...
Porcine Coronary Artery Smooth Muscle Cells (PCASMC) (>500,000 cells)...
Biology Products: