In order to compare yields between our kits and the leading competitor, side-by-side extractions were performed on aliquots from single cultures of various strains of E.coli containing different plasmids. The results are shown in Table 1. Clearly, the Perfectprep kits significantly outperformed the leading competitor's kit in terms of yield every time.
Table 1: Perfectprep Midi vs. Brand X
|Plasmid/Host||Culture Volume||PP Midi Yield||Brand X Yield||Average PP percentage of Leading Brand||Overall average PP percentage of Brand X|
|pEGFP-N1/XL-1 Blue||25 ml||201 g||133 g||147%||344%|
|185 g||128 g|
|pGEMgapdh/HB101 TD>||25 ml||137 g||27 g||592%|
|155 g||22 g|
|pCH110/JM109||75 ml||80 g||34 g||292%|
|148 g||44 g|
1) Sample 1, PP-Midi, uncut; 2) Sample 1, PP-Midi, 1U EcoR1; 3) Sample 1, PP-Midi, 5U EcoR1; 4) Sample 2, PP-Midi, uncut; 5) Sample 2, PP-Midi, 1U EcoR1; 6) Sample 2, PP-Midi, 5U EcoR1; 7) Sample 1, Brand X, uncut; 8) Sample 1, Brand X, 1U EcoR1; 9) Sample 1, Brand X, 5U EcoR1; 10) Sample 2, Brand X, uncut; 11) Sample 2, Brand X, 1U EcoR1; 12) Sample 2, Brand X, 5U EcoR1; 13) Lambda HindIII DNA Marker
Recover more DNA in less time!
ectprep Plasmid isolation kits offer a convenient, time and cost-effective
method for isolating plasmid DNA. Because each lot is performance tested
and the isolated DNA evaluated for quantity and quality using spectrophotometric
assay, digestion with restriction enzymes, automated fluorescent DNA cycle
sequencing, and transfection of cultured cells, the researcher is guaranteed
excellent quality, RNA-free plasmid DNA comparable to DNA purified using
a CsCl gradient. Typical follow-up applications include manual and automated
fluorescent sequencing, transfection, cloning, in vitro transcription,
and even microinjection.