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Eppendorf Perfectprep Plasmid DNA Isolation Kits

Eppendorf Perfectprep Plasmid DNA Isolation Kits are designed to generate high yields of highly pure plasmid DNA suitable for automated fluorescent sequencing, transfection, PCR, and other extremely sensitive downstream applications.

In order to compare yields between our kits and the leading competitor, side-by-side extractions were performed on aliquots from single cultures of various strains of E.coli containing different plasmids. The results are shown in Table 1. Clearly, the Perfectprep kits significantly outperformed the leading competitor's kit in terms of yield every time.

Table 1: Perfectprep Midi vs. Brand X

Plasmid/Host	Culture Volume	PP Midi Yield	Brand X Yield	Average PP percentage of Leading Brand	Overall average PP percentage of Brand X
pEGFP-N1/XL-1 Blue	25 ml	201 g	133 g	147%	344%
		185 g	128 g
pGEMgapdh/HB101	25 ml	137 g	27 g	592%
		155 g	22 g
pCH110/JM109	75 ml	80 g	34 g	292%
		148 g	44 g

Next we tested product purity by restriction digests of 250 ng of the pCH110 DNA. As is shown in figure 1, all samples cut to completion with both kits. Figure 1: Perfectprep Midi DNA and Brand X DNA Restriction Digest Restriction digests using EcoR1 from NEB were performed on 250 ng of the pCH110 DNA samples tabulated above. Lane order is as follows:

1) Sample 1, PP-Midi, uncut; 2) Sample 1, PP-Midi, 1U EcoR1; 3) Sample 1, PP-Midi, 5U EcoR1; 4) Sample 2, PP-Midi, uncut; 5) Sample 2, PP-Midi, 1U EcoR1; 6) Sample 2, PP-Midi, 5U EcoR1; 7) Sample 1, Brand X, uncut; 8) Sample 1, Brand X, 1U EcoR1; 9) Sample 1, Brand X, 5U EcoR1; 10) Sample 2, Brand X, uncut; 11) Sample 2, Brand X, 1U EcoR1; 12) Sample 2, Brand X, 5U EcoR1; 13) Lambda HindIII DNA Marker

Recover more DNA in less time!

Conclusion

Perfectprep Plasmid isolation kits offer a convenient, time and cost-effective method for isolating plasmid DNA. Because each lot is performance tested and the isolated DNA evaluated for quantity and quality using spectrophotometric assay, digestion with restriction enzymes, automated fluorescent DNA cycle sequencing, and transfection of cultured cells, the researcher is guaranteed excellent quality, RNA-free plasmid DNA comparable to DNA purified using a CsCl gradient. Typical follow-up applications include manual and automated fluorescent sequencing, transfection, cloning, in vitro transcription, and even microinjection.

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