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Eppendorf Perfectprep Gel Cleanup Kit

Eppendorf Perfectprep Gel Cleanup Kit

Jennifer Diers, Jody Davis, Christopher Bock, and Jose Gomez
Eppendorf 5 Prime, Boulder, Colorado Abstract

The purity of DNA extracted from agarose gels can have a significant impact on downstream applications. DNA fragments extracted and purified using the are free of salts, ethidium bromide, protein, agarose and organics, thereby allowing successful results in downstream applications. The provides a method for extraction of DNA from agarose gels for subsequent use in PCR*, sequencing, labeling and hybridization. DNA can be extracted from low and high melting temperature agarose in TBE or TAE buffer. This method has been optimized for purification of DNA fragments from 70 bp to 10 kb. Gel purification and binding capacity experiments (Fig. 1) show over 80% recovery of DNA when samples are processed using the .

Experiment # Ave. Yield (g)
(n=3) Std. Dev, 1 Unpurified Stock
Eppendorf
Brand Q 9.82
9.08
7.34
0.39
0.41 2 Unpurified Stock
Eppendorf
Brand Q 9.82

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