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Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

align="right">6
T=72.0 0:00:30
Hold 72C for 30 seconds
Final elongation temperature
7
SOUND 05
Beep 5 times
Notification of program end- add Stop Solution
8
HOLD 4 ENTER
Hold 4C
User must end program by STOP/ ENTER
END
Use SEL key to change, add, or delete a program line.
Use OPT key to view all parameters (i.e. ramp rate).
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