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Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

NO WAIT AUTO
Block temperature control

Heated lid temperature

Program starts immediately
Lid shuts off at end of program
As instructed in Pharmacias Cycle
Sequencing Kit, preheat block to 95C:

Version 2.1 or above use the incubate mode
Versions 2.1 or less must include a T= 95C
HOLD step prior to step 1 remember to alter
the GO TO step accordingly!
1
T=95.0 0:02:00
+0.00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 95C for 2 min.
Change ramp rate to 1C/ sec
Initial denaturation temperature ramping at
1C/ second
2
T=95.0 0:00:30
+0
'"/>

Source:


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