Navigation Links
Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

From MAIN MENU go to FILES/ EDIT/ STANDARD rewrite accordingly then EXIT/ YES (use SEL to choose)/ ENTER/ change name to BIGDYE/ ENTER
Step
Parameter
Explanation
Additional Comments



CNTRL= TUBE LID= 105 NO WAIT AUTO
Block temperature control

Heated lid temperature

Program starts immediately
Lid shuts off at end of program
As instructed in Pharmacias Cycle
Sequencing Kit, preheat block to 95C:

Version 2.1 or above use the incubate mode
Versions 2.1 or less must include a T= 95C
HOLD step prior to step 1 remember to alter
the GO TO step accordingly!
1
T=95.0 0:02:00
+0.00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 95C for 2 min.
Change ramp rate to 1C/ sec
Initial denaturation temperature ramping at
1C/ second
2
T=95.0 0:00:30
+0 .00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 95C for 30 sec.
Change ramp rate to 1C/ sec
Denaturation temperature ramping at 1C/
second
3*
T=55.0 0:00:36
+0.00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 55C for 36 sec.
Change ramp rate to 1C/ sec
Annealing temperature ramping at 1C/
second
4
T=72.0 0:00:30
+0.00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 72C for 30 sec.
Elongation temperature ramping at 1C/
second
5
GO TO 2 REP 24
Repeat cycle 24 times
Step 2-4, N = 25
6
T=72.0 0:00:30
Hold 72C for 30 seconds
Final elongation temperature
7
SOUND 05
Beep 5 times
Notification of program end- add Stop Solution
8
HOLD 4 ENTER
Hold 4C
User must end program by STOP/ ENTER
END
Use SEL key to change, add, or delete a program line.
Use OPT key to view all parameters (i.e. ramp rate).
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
2. Eppendorf UVette Material Compatibility
3. Eppendorf Perfectprep Gel Cleanup Kit
4. Eppendorf Perfectprep Gel Cleanup Kit
5. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
6. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
7. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
8. EppendorfPerfect gDNA Blood Mini Kit
9. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. EppendorfPerfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/11/2017)... ... August 11, 2017 , ... “There is an ... natural alternatives to synthetic ingredients,” said Matt Hundt, President of Third Wave Bioactives. ... manufacturing presence and know-how of Biorigin will allow us to bring truly novel ...
(Date:8/10/2017)... Ca (PRWEB) , ... August 09, 2017 , ... ... for regenerative medicine applications in the clinic is here. The team at Capricor ... present in conditioned medium for clinical studies. , Dr. Travis Antes, head ...
(Date:8/10/2017)... (PRWEB) , ... August 09, 2017 , ... ... with four international biomedical optics laboratories — the Wellman Center for Photomedicine, the ... Laser Center Lübeck and the Beckman Laser Institute at University of California, Irvine ...
(Date:8/10/2017)... (PRWEB) , ... August 10, 2017 , ... ... the stock market news outlet had initiated coverage on Next Group Holdings, Inc. ... and underserved consumer markets geared toward those that cannot engage in traditional banking ...
Breaking Biology Technology:
(Date:4/5/2017)... KEY FINDINGS The global market ... CAGR of 25.76% during the forecast period of 2017-2025. ... for the growth of the stem cell market. ... MARKET INSIGHTS The global stem cell market is segmented ... The stem cell market of the product is segmented ...
(Date:3/30/2017)... LOS ANGELES , March 30, 2017  On ... Hack the Genome hackathon at ... This exciting two-day competition will focus on developing health ... experience. Hack the Genome is ... has been tremendous. The world,s largest companies in the ...
(Date:3/29/2017)...  higi, the health IT company that operates the ... , today announced a Series B investment from ... The new investment and acquisition accelerates higi,s strategy to ... population health activities through the collection and workflow integration ... collects and secures data today on behalf of over ...
Breaking Biology News(10 mins):