Navigation Links
Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

From MAIN MENU go to FILES/ EDIT/ STANDARD rewrite accordingly then EXIT/ YES (use SEL to choose)/ ENTER/ change name to BIGDYE/ ENTER
Step
Parameter
Explanation
Additional Comments



CNTRL= TUBE LID= 105 NO WAIT AUTO
Block temperature control

Heated lid temperature

Program starts immediately
Lid shuts off at end of program
As instructed in Pharmacias Cycle
Sequencing Kit, preheat block to 95C:

Version 2.1 or above use the incubate mode
Versions 2.1 or less must include a T= 95C
HOLD step prior to step 1 remember to alter
the GO TO step accordingly!
1
T=95.0 0:02:00
+0.00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 95C for 2 min.
Change ramp rate to 1C/ sec
Initial denaturation temperature ramping at
1C/ second
2
T=95.0 0:00:30
+0 .00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 95C for 30 sec.
Change ramp rate to 1C/ sec
Denaturation temperature ramping at 1C/
second
3*
T=55.0 0:00:36
+0.00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 55C for 36 sec.
Change ramp rate to 1C/ sec
Annealing temperature ramping at 1C/
second
4
T=72.0 0:00:30
+0.00 +0:00
R=1.0/s +0.0/s
G=00.0
Hold 72C for 30 sec.
Elongation temperature ramping at 1C/
second
5
GO TO 2 REP 24
Repeat cycle 24 times
Step 2-4, N = 25
6
T=72.0 0:00:30
Hold 72C for 30 seconds
Final elongation temperature
7
SOUND 05
Beep 5 times
Notification of program end- add Stop Solution
8
HOLD 4 ENTER
Hold 4C
User must end program by STOP/ ENTER
END
Use SEL key to change, add, or delete a program line.
Use OPT key to view all parameters (i.e. ramp rate).
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
2. Eppendorf UVette Material Compatibility
3. Eppendorf Perfectprep Gel Cleanup Kit
4. Eppendorf Perfectprep Gel Cleanup Kit
5. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
6. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
7. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
8. EppendorfPerfect gDNA Blood Mini Kit
9. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. EppendorfPerfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/18/2017)... -- Research and Markets has announced the addition of ... their offering. ... The report provides separate comprehensive analytics for the US, ... World. Annual estimates and forecasts are provided for the period 2015 ... markets. Market data and analytics are derived from primary and secondary ...
(Date:2/17/2017)... ... February 17, 2017 , ... Academy of Model Aeronautics (AMA), ... maker of unmanned aircraft systems (UAS), are launching a joint program to promote ... and support educational outreach efforts. , AMA and DJI will collaborate on other ...
(Date:2/16/2017)... , Feb. 16, 2017  ImMAGE Biotherapeutics (OTCMKTS: IMMG), an ... to find a better treatment for triple negative breast cancer ... France program. The YEi ... accelerator designed to help entrepreneurs grow their business in ... of eight American companies selected to complete an intensive one ...
(Date:2/16/2017)... DUBLIN , Feb. 16, 2017 ... the "Synthetic Biology: Global Markets" report to ... ... synthetic-biology products (synthetic genes, biobrick parts, delivery plasmids, chassis ... DNA synthesis and assembly, genome editing, bioinformatics and specialty ...
Breaking Biology Technology:
(Date:2/10/2017)... -- Research and Markets has announced the ... Scientific and Commercial Aspects" to their offering. ... Biomarkers play ... therapy for selection of treatment as well for monitoring the ... disease in modern medicine. Biochip/microarray technologies and next generation sequencing ...
(Date:2/8/2017)... Feb. 7, 2017 Report Highlights ... The global synthetic-biology market ... billion by 2021, growing at a compound annual growth rate ... overview of the global markets for synthetic biology. - Analyses ... 2016, and projections of compound annual growth rates (CAGRs) through ...
(Date:2/7/2017)... , Feb. 7, 2017 Zimmer Biomet ... in musculoskeletal healthcare, will present at the LEERINK Partners ... York Palace Hotel on Wednesday, February 15, 2017 at ... webcast of the presentation can be accessed at ... following the conference via Zimmer Biomet,s Investor Relations website ...
Breaking Biology News(10 mins):