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Epitope-Tagging Vectors for Functional Analysis in Yeast

gure 4

Conclusions

With Stratagenes new series of epitope-tagging vectors to express and functionally analyze eukaryotic genes in S. cerevisiae, high protein levels can be detected using available antibodies against the epitope tags.

Acknowledgments

The authors thank Edward Aranas, Alan Greener, Mary Buchanan, and John Bauer at Stratagene for discussions and suggestions.

REFERENCES
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  4. Johnston, M. (1977) Microbiol. Rev. 51: 458-476.

  5. Marathe, S. V. and McEwen, J. E. (1995) Gene 154: 105-107.

  6. Lohr, D., Venkov, P., and Zlatanova, J. (1995) FASEB J. 9: 777-787.

  7. Hopp, H.P., et al. (1988) Bio/Technology 6: 1204-1210.

  8. Evan, G. I., et al. (1985) Mol. Cell. Biol. 5: 3610-3616.

  9. Kolodziej, P. A. and Young, R. A. (1991) Methods Enzymol. 194: 508-519.

  10. Zealey, G. R., et al. (1988) Mol. Gen. Genet. 211: 155-159.

The FLAG Technology is under license from Sigma-Aldrich Co.


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