Navigation Links
Epitope-Tagging Vectors for Functional Analysis in Yeast

ucMC or pESC-URA-LucFN were selected on SD-URA plates.

Figure 3

The transformants were grown in selective media with glucose or galactose as the carbon source, and the cleared cell lysate was assayed for luciferase activity. As shown in Figure 3, all eight constructs expressed active luciferase. When pESC-TRP-LucFN or pESC-TRP-LucMC was used, under induced conditions, the activity of the epitope-tagged luciferase showed a level similar to that of the untagged luciferase (Figure 2). The data indicate that the epitope-tagged luciferase retains its biological activity, and the tags do not interfere with the enzyme activity. We consistently observed that the luciferase activities derived from the pESC-URA vector are approximately two- to three-fold less, compared to those derived from other pESC vectors (Figure 3). Lower levels of luciferase expression were most likely due to the URA3 selectable marker contained in the vector. While the HIS3, LEU2, and TRP1 gene products are involved in amino acid biosynthesis, the URA3 gene product is involved in uracil biosynthesis. Marker gene activity has been shown to influence vector maintenance and levels of gene expression in yeast.10 To detect the epitope-tagged luciferase, we subjected the yeast lysates to Western blot analysis using antibodies specific to the epitope tag. A 64-kDa band, corresponding to the expected size of the epitope-tagged luciferase protein, was detected in samples derived from cells grown in galactose media (Figure 4, even lanes) but not in samples from cells grown in glucose media (Figure 4, odd lanes).

Fi
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
4. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. Versatile Reporter Vectors for Monitoring Viral Transduction
7. High-Titer Retroviral Vectors for Gene Delivery
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Mammalian Expression Vectors with Interchangeable Selectable Markers
10. Delivering siRNA Using Adenoviral Vectors
11. Drive Long-Term Silencing with CMV Vectors

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Epitope Tagging Vectors for Functional Analysis Yeast

(Date:12/19/2014)... OR and Hershey, PA (PRWEB) December 19, 2014 ... Pink: BJCT), a leading developer and manufacturer of ... entered into an agreement with Immunomic Therapeutics, Inc. ... Biojector®-2000 needle-free injection device with its LAMP™ vaccine ... an option for an exclusive Worldwide license to ...
(Date:12/19/2014)... MA (PRWEB) December 19, 2014 Charm ... MRLAFMQ test for the Detection of Aflatoxin M1 in ... to receive independent third party validation. The peer reviewed ... Unit of the Institute for Agricultural and Fisheries Research ... Group. , Aflatoxin B1, the most toxic aflatoxin ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), ... promotion of Nick Maroulis, Pharm.D. to the newly created ... , In this position, Dr. Maroulis will continue to ... directors of our multi-site pharmacies as the company expands. ... during that time he has served in many different ...
(Date:12/19/2014)... EVANSTON, Ill. , Dec. 19, 2014 ... to develop novel drugs for diseases of the central ... Ph.D., president and chief executive officer, will present at ... Dr. Riedel,s presentation will take place at 3:00 p.m. ... St. Francis in San Francisco, Calif. ...
Breaking Biology Technology:Bioject and Immunomic Therapeutics Enter into an Agreement for License of Needle-Free Technology for LAMP-vax Vaccines 2Bioject and Immunomic Therapeutics Enter into an Agreement for License of Needle-Free Technology for LAMP-vax Vaccines 3Charm Sciences Achieves Independent Validation of First 15 Minute Quantitative Screening Method for Aflatoxin M1 2BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Naurex to Present at 33rd Annual J.P. Morgan Healthcare Conference 2
... non-profit arm the SBC Foundation, has given $285,500 in ... Milwaukee has reported. , ,Twenty-four organizations that ... received money to help them use more technology. The ... which typically gives from $2,500 to $25,000 per organization. ...
... secretary of Wisconsins Department of Commerce, said on Friday that he ... partner in the law firm Quarles & Bradys Milwaukee office. , ... two years. He will be returning to the firm he worked ... deal from Governor Doyle as a leader, a boss, and a ...
... Having judged last years a success, the Wisconsin ... the second annual Wisconsin Governor's Business Plan Contest ... businesses will submit 250-word business proposals within one of ... business services. The proposals will be accepted until January ...
Cached Biology Technology:Secretary Nettles leaving Commerce for law practice 2Secretary Nettles leaving Commerce for law practice 3Governors business-plan competition starts another round 2Governors business-plan competition starts another round 3
(Date:12/19/2014)... Research and Markets ( http://www.researchandmarkets.com/research/8kmfcn/iphone_5s ... "iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & ... offering. http://photos.prnewswire.com/prnh/20130307/600769 ... July 2012, Apple introduced the fingerprint reading feature ... only device of Apple incorporating such a feature, ...
(Date:12/19/2014)... --Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has ... North America Perimeter Security Systems Market" report to ... The North American perimeter security market is ... 2014 to 2019. Although the U.S. market holds a ... is expected to grow at a higher CAGR ...
(Date:12/19/2014)... , Dec. 18, 2014  23andMe, Inc., the leading ... study that pinpoints fine-scale differences in genetic ancestry of ... . Since immigrants first arrived more than ... has served as a meeting place for ... history and the ongoing mixing of peoples with African, ...
Breaking Biology News(10 mins):iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 323andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4
... , Sept. 4, 2013  Rapid growth in microRNA (miRNA) ... its diagnostic and therapeutic potential, has fuelled development ... Of the various miRNA technologies, which include quantitative ... functional tools, qRT PCR remains the fastest-growing, while ...
... . Families of thousands of victims of ... appropriate punishment, because of a suite of technologies able ... graves, perhaps hundreds of thousands remain undiscovered. Researchers at ... Ridge National Laboratory are developing a method to discover ...
... pleased to announce that the 2014 HFSP Nakasone Award ... Institute of Science for his pioneering work in discovering ... single most important foundation of the field of systems ... of genetic circuits to systematic analysis and understanding. ...
Cached Biology News:Frost & Sullivan: Diagnostic and Therapeutic Potential of microRNAs Stimulate Need for More Research Tools 2Frost & Sullivan: Diagnostic and Therapeutic Potential of microRNAs Stimulate Need for More Research Tools 3Frost & Sullivan: Diagnostic and Therapeutic Potential of microRNAs Stimulate Need for More Research Tools 4September 2013 story tips 2September 2013 story tips 3The 2014 HFSP Nakasone Award goes to Uri Alon 2
... The SPD101B is a no-frills, basic SpeedVac ... Although economically priced, this unit still features ... a digitial display of time and heat. ... samples and accepts a wide variety of ...
Digital display of time, vacuum and temperature , Dual vacuum control of ramping rate and ultimate level , Comes complete with RH40-12 (40 x 1.5 ml) Rotor and (2) Glass Condensation Flasks. ...
... The DNA120 is a dedicated concentrator for ... precipitates of DNA/RNA. The integrated system combines ... pump. The compact design, automatic pump and ... run time; one for heater time), makes ...
For absolute protein quantification. 4 peptides in 5 vials x 2 nmol per peptide...
Biology Products: