Navigation Links
Epitope-Tagging Vectors for Functional Analysis in Yeast

nces: a ColE1ori-ampR fragment, which allows antibiotic selection and replication of the vector in E. coli for cloning; the 2-m sequence, which provides the origin of replication so that the vector can replicate autonomously in S. cerevisiae; an auxotrophic selectable marker gene (HIS, LEU2, TRP1, or URA3) to select and maintain the expression vector in yeast cells; GAL1 and GAL10 promoters in opposite orientation; a multiple cloning site (MCS); and a transcription termination sequence downstream of each promoter.

LEU2, TRP1, and HIS3 genes encode b-isopropylmalate dehydrogenase, N-(5-phosphoribosyl) anthranilate isomerase, and imidizoleglycerolphosphate dehydratase, respectively. These gene products are required for the biosynthesis of the amino acids leucine, tryptophan, and histidine, respectively. The URA3 gene encodes orotidine-5-phosphate decarboxylase, which is required for uracil biosynthesis. Plasmids bearing URA3 can be counter-selected by plating the cells on media containing 5-FOA (5-fluoroorotic acid). These features facilitate the functional analysis of genes by plasmid shuffling.3

Both the GAL1 and GAL10 promoters from S. cerevisiae are strictly regulated at the transcription level by the carbon source in the media. These promoters are tightly repressed when glucose is present in the media and are highly induced when galactose is the sole carbon source.4 In S. cerevisiae, the induction ratio of these promoters has been estimated to be greater than 1000 fold.5,6 The presence of both the GAL1 and GAL10 promoters in opposite orientation allows two genes to coexpress in the same host cell.

The pESC vectors also contain DNA sequences coding for epitope peptid
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
4. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. Versatile Reporter Vectors for Monitoring Viral Transduction
7. High-Titer Retroviral Vectors for Gene Delivery
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Mammalian Expression Vectors with Interchangeable Selectable Markers
10. Delivering siRNA Using Adenoviral Vectors
11. Drive Long-Term Silencing with CMV Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Epitope Tagging Vectors for Functional Analysis Yeast

(Date:9/18/2014)... , Sept. 18, 2014  Health Enhancement Products, Inc. ... investigating early-stage algae bioactive compounds and metabolic processes, is pleased ... company,s board of directors. Ms. Nola E. Masterson , ... advisor joined the board effective September 17, 2014. ... agree to join our board," states Andrew Dahl , ...
(Date:9/18/2014)... 2014 On Tuesday, Secretary of Energy ... Risch for National Lab Day on the Hill. The ... national laboratory system. Durbin and Risch also formally launched ... awareness of the reach of the national labs as ... address some of our nation's most pressing challenges. , ...
(Date:9/18/2014)... -- Research and Markets has announced the ... 2014" report to their offering. ... professional and in-depth study on the current state of ... provides a basic overview of the industry including definitions, ... market analysis is provided for the international markets including ...
(Date:9/18/2014)... 2014  U.S. biotech company Kultevat has ... for technology developed by the Donald Danforth Plant ... including for applications in production of natural rubber in ... was developed under the direction of Roger Beachy ... president of the Danforth Plant Science Center, and is ...
Breaking Biology Technology:Health Enhancement Announces New Board Member In Advance Of Annual Shareholder Meeting 2Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 2Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 3Global Human Albumin Industry Report 2014 2Global Human Albumin Industry Report 2014 3Kultevat obtains license of gene switch technology 2
... magnetic fields and tiny iron-bearing particles to drive healthy ... done in animals, may lead to a new method ... diseased organs in people. , The study team, led ... Chair of Pediatric Cardiology at The Childrens Hospital of ...
... BALTIMORE, Jan. 7 Physicians and ... California will benefit from,a new partnership ... University,Medical Center, providing resources to help ... thereby improving patient care., (Logo: ...
... 7 Cephalon, Inc. (Nasdaq:,CEPH) announced today that the ... the JPMorgan Healthcare Conference are now available on the,"Investor ... On Tuesday, January 8, 2008, Frank Baldino, Jr.,Ph.D., Chairman ... the,Conference beginning at 2:00 p.m. Pacific Time (5 p.m. ...
Cached Biology Technology:Researchers use magnetism to target cells to animal arteries 2Researchers use magnetism to target cells to animal arteries 3Physicians Practice, Loma Linda Partner to Improve Patient Care in Southern California 2Cephalon Presentation Slides for the 26th Annual JPMorgan Healthcare Conference Now Available 2
(Date:9/18/2014)... deep in the primitive brainstem has revealed how we ... School of Medicine and the University at Buffalo School ... second "sleep node" identified in the mammalian brain whose ... produce deep sleep. , Published online in August in ... half of all of the brain,s sleep-promoting activity originates ...
(Date:9/18/2014)... research into the Crimean-Congo hemorrhagic fever virus (CCHFV), a ... humans similar to that caused by Ebolavirus, has identified ... has the potential to lead to novel targets for ... research, reported in a paper published today in the ... at the Texas Biomedical Research Institute and their colleagues, ...
(Date:9/18/2014)... and the Quebec government have discovered microplastics (in ... of Fisheries and Aquatic Sciences . , The ... industrial cleansers, to which they are commonly added ... buoyancy, they may readily pass through sewage treatment ... the world,s oceans, but have only recently been ...
Breaking Biology News(10 mins):No sedative necessary: Scientists discover new 'sleep node' in the brain 2Research milestone in CCHF virus could help identify new treatments 2Microplastic pollution discovered in St. Lawrence River sediments 2
... have fish-like sensations, advanced ship design and more, four ... on Dec. 23 learned they will receive the nation,s ... researchers have been selected for Presidential Early Career Awards ... at a ceremony at the White House early this ...
... forces in a quest to correct a faulty chromosome through ... , used stem cells to correct a defective "ring chromosome" ... correct chromosome abnormalities that give rise to birth defects, mental ... may be possible to use this approach to take cells ...
... a key difference in the biological mechanisms by which the ... study, published in the journal Nature Immunology and ... Life Sciences, centred on STAT1, a protein that can bind ... in the body. STAT-1 responds to interferon signals, hormone-like ...
Cached Biology News:White House lauds ONR-funded researchers for early success 2Nature study discovers chromosome therapy to correct a severe chromosome defect 2Nature study discovers chromosome therapy to correct a severe chromosome defect 3Research uncovers key difference between our bodies' fight against viruses and bacteria 2
Mouse monoclonal antibody raised against a partial recombinant FBXO11. NCBI Entrez Gene ID = FBXO11...
Mouse polyclonal antibody raised against a partial recombinant LASS3. NCBI Entrez Gene ID = 204219...
... Antibody against Nonmuscle Myosin II Heavy ... (Stored in TBS + 0.03% Thimerosal) ... peptide SDVNETQPPQSE corresponding to the C-terminus ... B (MHC-B) This antibody is effective ...
... Anti-Mi-2 Immunogen : ... acid residues 474-970 of human ... 7.4, 0.105M NaCl, 0.035% sodium ... Assurance: routinely evaluated by immunoblot ...
Biology Products: