Navigation Links
Epitope-Tagging Vectors for Functional Analysis in Yeast

nces: a ColE1ori-ampR fragment, which allows antibiotic selection and replication of the vector in E. coli for cloning; the 2-m sequence, which provides the origin of replication so that the vector can replicate autonomously in S. cerevisiae; an auxotrophic selectable marker gene (HIS, LEU2, TRP1, or URA3) to select and maintain the expression vector in yeast cells; GAL1 and GAL10 promoters in opposite orientation; a multiple cloning site (MCS); and a transcription termination sequence downstream of each promoter.

LEU2, TRP1, and HIS3 genes encode b-isopropylmalate dehydrogenase, N-(5-phosphoribosyl) anthranilate isomerase, and imidizoleglycerolphosphate dehydratase, respectively. These gene products are required for the biosynthesis of the amino acids leucine, tryptophan, and histidine, respectively. The URA3 gene encodes orotidine-5-phosphate decarboxylase, which is required for uracil biosynthesis. Plasmids bearing URA3 can be counter-selected by plating the cells on media containing 5-FOA (5-fluoroorotic acid). These features facilitate the functional analysis of genes by plasmid shuffling.3

Both the GAL1 and GAL10 promoters from S. cerevisiae are strictly regulated at the transcription level by the carbon source in the media. These promoters are tightly repressed when glucose is present in the media and are highly induced when galactose is the sole carbon source.4 In S. cerevisiae, the induction ratio of these promoters has been estimated to be greater than 1000 fold.5,6 The presence of both the GAL1 and GAL10 promoters in opposite orientation allows two genes to coexpress in the same host cell.

The pESC vectors also contain DNA sequences coding for epitope peptid
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
4. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. Versatile Reporter Vectors for Monitoring Viral Transduction
7. High-Titer Retroviral Vectors for Gene Delivery
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Mammalian Expression Vectors with Interchangeable Selectable Markers
10. Delivering siRNA Using Adenoviral Vectors
11. Drive Long-Term Silencing with CMV Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Epitope Tagging Vectors for Functional Analysis Yeast

(Date:8/29/2014)... Antonio, TX (PRWEB) August 29, 2014 ... and ISO 13485:2003 certified, GAMP® 5 compliant imaging ... awarded an international Phase II clinical trial to ... lymphoma. , Throughout this trial, Intrinsic Imaging will ... not limited to, protocol and charter development, site ...
(Date:8/28/2014)... “This kit has an improved extraction efficiency and ... 150 ppb,” said Mark Tess, PhD, Mycotoxin Product Manager for ... owners and USDA-GIPSA inspection agencies that have a need to ... place in a matter of minutes with nothing but our ... feed and grain before accepting it, but they no longer ...
(Date:8/28/2014)... August 28, 2014 The ability for ... culture that will allow individuals to perform at the ... important for employers looking to compete in this post–recession ... embracing this trend, as the sector increasingly focuses on ... company, which will have real results on the organization's ...
(Date:8/28/2014)... Aug. 28, 2014  Armetheon, Inc. ( www.armetheon.com ... mid- to late-stage cardiovascular drug candidates, today announced ... first round of financing. The Series A round ... Bioventures with participation from investors that included Atheneos ... Dr. Larry Hsu , the founder of ...
Breaking Biology Technology:Intrinsic Imaging Awarded Phase II Clinical Trial to Assess New Treatment for Non-Hodgkin’s Lymphoma 2Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 2Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 3Armetheon Completes $7.0 Million Series A Financing 2Armetheon Completes $7.0 Million Series A Financing 3
... China, FORT LAUDERDALE, Fla., Sept. 5 ... pioneer in laser,optical breast cancer imaging systems, has ... Tianjin Medical University,s Cancer Institute and,Hospital, the largest ... http://www.newscom.com/cgi-bin/prnh/20040412/IMDSLOGO ) Research at the Cancer ...
... EDMONTON, Sept. 5 /PRNewswire-FirstCall/ - Biomira Inc. (Nasdaq: ... of three-year survival results,from a randomized Phase II ... lung cancer (NSCLC). The results suggest that Stimuvax,combined ... benefits to,patients with unresectable stage IIIB NSCLC who ...
... FRANCISCO, Calif., Sept. 5 Hyperion,Therapeutics, a biopharmaceutical ... and hepatology therapies for the,treatment of Urea Cycle ... $40 million Series B financing. Leading the round ... Partners and,NEA, all of Menlo Park, CA. WRF ...
Cached Biology Technology:Largest Asian Cancer Hospital to Evaluate New CT Laser Breast Imaging System 2Largest Asian Cancer Hospital to Evaluate New CT Laser Breast Imaging System 3Stimuvax(R) Phase II data highlight three-year survival results for patients with non-small cell lung cancer 2Stimuvax(R) Phase II data highlight three-year survival results for patients with non-small cell lung cancer 3Stimuvax(R) Phase II data highlight three-year survival results for patients with non-small cell lung cancer 4Hyperion Therapeutics Secures $40 Million In Series B Round 2
(Date:8/28/2014)... than traditional cigarettes, but still release toxins into ... USC. , Scientists studying secondhand smoke from e-cigarettes ... harmful particles, with close-to-zero exposure to organic carcinogens. ... in second-hand e-cigarette smoke were found to be ... levels of polycyclic aromatic hydrocarbons cancer-causing organic ...
(Date:8/28/2014)... groups and clinicians is critical to the development ... of Georgia research. , A qualitative study of ... that role models for successful breastfeeding help positively ... "Mothers who received that support are more likely ... Alex Anderson, an associate professor in the College ...
(Date:8/28/2014)... complex systems. Their infrastructure equipment requires ancillary sub-systems ... to supply fuel, and ICT systems for control ... is interconnected with a wider network and they ... scientists has studied various aspects of inter-network dependencies, ... in EPJ B by Gaihua Fu from Newcastle ...
Breaking Biology News(10 mins):Second-hand e-cig smoke compared to regular cigarette smoke 2Breastfeeding study shows need for effective peer counseling programs 2Inter-dependent networks stress test 2
... by Dr. Ann F. Jacobson, associate professor in Kent ... may initially choose to postpone but ultimately undergo total ... patient education before and after the procedure. ... pre-and post operative experiences with total knee replacement surgery, ...
... release is available in Spanish . , A ... (USA) and Nakai (China), among others, have deciphered for the first ... to the cover of the last issue of the prestigious journal ... to the group of Molecular Genetics of the Department of ...
... fund four faculty research projects for spring 2008. ... a number of excellent proposals submitted in response ... for the program is administered by the OSU ... by royalties from OSU-licensed technologies. These funded projects ...
Cached Biology News:Research examines factors in delaying or declining total knee replacement surgery 2Scientists decipher fruit tree genome for the first time 2Scientists decipher fruit tree genome for the first time 3OSU technologies funded for development 2OSU technologies funded for development 3
... Maxi MB (for aqueous, other low volatility ... systems respond to specific applications of molecular ... concentration of heat-labile samples. The RC systems ... cold traps and a wide selection of ...
... Acid Resistant SpeedVac Model SPD131DDA , Our ... SPD131DDA is designed to handle the rigors and ... slim design, digital display, dual independent timers, an ... chamber and vapor pathway. , , Adjustable ...
...
For absolute protein quantification. 4 peptides in 5 vials x 2 nmol per peptide...
Biology Products: