Navigation Links
Enzyme Purification With the Econo-Pac Q Cartridge

Data submitted by Dr. Ivan Kaiser, University of Wyoming, Laramie, WY USA


Summary
Dr. Ivan Kaisers research involves the isolation and purification of proteins from snake venom, such as post-synaptic neurotoxins and hemorrhagic factors. In addition to research, Dr. Kaiser teaches a course on protein purification, in which students study the principles of protein purification using snake venom as a model. Students use various separation techniques, such as ion exchange and gel filtration chromatography, and polyacrylamide gel electrophoresis.

Shown below are typical data from university classroom experiments. Two ion exchange columns are examined: an Econo-Pac Q cartridge and a hand-packed DEAE Sephadex column. As shown below, the Econo-Pac Q cartridge resolves peaks more efficiently than the user-packed column. (Note: The two gradient profiles are slightly different.)


Supporting Data and Procedure
Figure 1 shows the fractionation of Crotalus horridus horridus venom using a 2.5 x 15 cm DEAE Sephadex column. Crude, dried venom is dissolved in 50 mM Tris, pH 8.0, then filtered through a 0.45 m filter. Twenty milligrams are loaded onto a column, and the gradient is initiated. Fractions of 4.7 ml were manually collected, and the absorbance of each fraction was measured at 280 nm.

Figure 2 shows a similar separation using the 5 ml Econo-Pac Q cartridge on an FPLC system. Two milligrams of prepared snake venom are loaded onto a cartridge, and the gradient is initiated.

FPLC and Sephadex are trademarks of Pharmacia Fine Chemicals.


back to top
'"/>

Source:


Page: All 1

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Greater Amplification Specificity with New Hot Start PCR Enzyme
5. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
6. Improve Amplification Specificity with Hot Start PCR Enzyme
7. Choice of RT-PCR Enzymes
8. Properties of PCR Enzymes
9. Select the Optimal Enzyme for RT-PCR
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... 27, 2016  Sequenom, Inc. (NASDAQ: SQNM ... lives through the development of innovative products and services, ... the United States denied its petition to ... of Sequenom,s U.S. Patent No. 6,258,540 (",540 Patent") are ... by the Supreme Court,s Mayo Collaborative Services v. Prometheus ...
(Date:6/27/2016)... ... June 27, 2016 , ... Newly created 4Sight ... solutions to the healthcare market. The company's primary focus is on new product ... marketing strategies that are necessary to help companies efficiently bring their products to ...
(Date:6/27/2016)... --  Ginkgo Bioworks , a leading organism design ... awarded as one of the World Economic Forum,s ... innovative companies. Ginkgo Bioworks is engineering biology to ... in the nutrition, health and consumer goods sectors. ... including Fortune 500 companies to design microbes for ...
(Date:6/24/2016)... ... , ... While the majority of commercial spectrophotometers and fluorometers use the z-dimension ... are higher end machines that use the more unconventional z-dimension of 20mm. Z-dimension ... bottom of the cuvette holder. , FireflySci has developed several Agilent flow cell ...
Breaking Biology Technology:
(Date:4/13/2016)... CHICAGO , April 13, 2016  IMPOWER physicians ... are setting a new clinical standard in telehealth ... By leveraging the higi platform, IMPOWER patients can ... weight, pulse and body mass index, and, when they ... quick and convenient visit to a local retail location ...
(Date:3/31/2016)... 31, 2016  Genomics firm Nabsys has completed a ... Barrett Bready , M.D., who returned to the company ... technical leadership team, including Chief Technology Officer, John ... Steve Nurnberg and Vice President of Software and Informatics, ... Dr. Bready served as CEO of Nabsys ...
(Date:3/23/2016)... , March 23, 2016 ... erhöhter Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender ... das Unternehmen mit SpeechPro zusammenarbeitet, um erstmals ... Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, im Rahmen ...
Breaking Biology News(10 mins):