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Enzyme Immunoassay for Studying Intracellular Levels of cAMP

terest. In addition to Jurkat cells, AC toxin has also been shown to elevate cAMP in monocyte macrophages,10 polymorphonuclear leukocytes and alveolar macrophages,11 natural killer cells12 and HL-60 promyelocytic leukemia cells.13

Conclusions

The EIA cAMP Kit is a nonradioactive EIA system for measuring intracellular cAMP levels. The kit is simple, convenient and highly sensitive and allows determination of cAMP levels from cell culture or tissue preparations. Intracellular cAMP levels can be detected in the nanomolar or picomolar range. Stratagene also offers AC toxin, which significantly increases intracellular cAMP levels as an alternative to currently available cAMP agonists.

REFERENCES

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  2. Barnes, S.J., and Conn, P.M. (1993) Endocrinology 133: 2756-2760.

  3. Wells, J.N., et al. (1975) Mol. Pharmacol. 11: 775.

  4. Seamon, K.B., and Daly, J.W. (1986) Adv. Cyclic Nucleotide Protein Phosphorylation Res. 20: 1.

  5. Selfe, S., et al. (1987) Mol. Pharmacol. 31: 529-534.

  6. Hewlett, E.L., et al. (1982) J. Biol. Chem. 268: 7842-7848.

  7. Sebo, P., et al. (1991) Gene 104: 19-24.

  8. Confer, D.L., and Eaton, J.W. (1982) Science 217: 948-950.

  9. Gentile, F., et al. (1988) Eur. J. Biochem. 175: 447-453.

  10. Hewlett, E.L., et al. (1983) Clin. Res. 31: 365A.

  11. Hewlett, E.L., et al. (1989) J. Biol. Chem. 264: 19379-19389.

  12. Hewlett, E.L., et al. (1993) J. Bi
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