Navigation Links
Enzyme Immunoassay for Studying Intracellular Levels of cAMP

emoved by a wash step. The reaction is completed by the addition of the colorimetric substrate, 3,3,5,5-tetramethylbenzidine/hydrogen peroxide (TMB/H2O2). The addition of TMB/H2O2 to each well results in a blue-green product background, and the reaction is stopped by the addition of 1 M phosphoric acid. A bright yellow color results, and absorbance of peroxidase-labeled cAMP that is bound to the cAMP-specific antibody is determined using a spectrophoto- metric plate reader at 450 nm. Since the amount of cAMP-horseradish peroxidase bound to rabbit anti-cAMP antibody is inversely proportional to cAMP in the sample (or standard), a high absorbance value corresponds to low levels of cAMP present in the sample. The EIA cAMP Kit includes cAMP standards for calculating standard curves. As an option for increased sensitivity (figure 2), standards and prepared samples can be acetylated by treatment with acetic anhydride in the presence of triethylamine.1 Picomolar detection is possible for acetylated cAMP, whereas nonacetylated cAMP levels can generally be assayed in the nanomolar range. The EIA cAMP Kit includes all the re- agents and protocols for quantifying cAMP levels in both nonacetylated and acetylated preparations.

AC Toxin for Increased Intracellular cAMP Levels

Stratagene is offering adenylate cyclase (AC) toxin, a useful reagent for increasing intracellular cAMP levels in cultured cells. AC toxin can be used to study cAMP-dependent protein kinase activation, the key regulatory event in signal transduction pathways involving cAMP. Modulators currently available for examining the effects of cAMP on intracellular signaling include
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Greater Amplification Specificity with New Hot Start PCR Enzyme
5. Improve Amplification Specificity with Hot Start PCR Enzyme
6. Choice of RT-PCR Enzymes
7. Properties of PCR Enzymes
8. Select the Optimal Enzyme for RT-PCR
9. Enzyme Purification With the Econo-Pac Q Cartridge
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Enzyme Immunoassay for Studying Intracellular Levels cAMP

(Date:9/30/2014)... Nematicide is a type of chemical ... parasitic roundworms, found in massive quantities virtually everywhere—in ... animals. Nematicides have tended to be broad-spectrum toxicants ... through the soil. A single gram of soil ... more than 10,000 species classified, they are one ...
(Date:9/30/2014)... 2014 Follow us on LinkedIn ... type of cholesterol carrier or lipoprotein in the blood. ... cholesterol levels in the body, especially along the walls ... attack and other cardiovascular disorders. Cardiovascular Diseases (CVD) is ... cholesterol levels on a regular basis, to assess an ...
(Date:9/30/2014)... Fast, semi-automatic creation of ... documents ChemAxon , a leader ... for life science research, launches ChemCurator, an application ... from documents. Markush structures are essential ... and understanding Markush structures is a difficult and ...
(Date:9/30/2014)... CORALVILLE, Iowa , Sept. 30, 2014 /PRNewswire/ ... in the discovery and development of proprietary new ... United States Patent and Trademark Office (USPTO) issued ... patent application entitled, "Benzoic Acid, Benzoic Acid Derivatives ... Methods of Making and Use Thereof." ...
Breaking Biology Technology:Nematicides Market by Types (Fumigants, Organophosphates, Carbamate and Bio-Nematicides), Applications (Agrochemicals, Industrial, and Others), and Geography (North America, Europe, Asia-Pacific & ROW) - Global Trends & Forecasts to 2019 2Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 2Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 3Surging Diabetic, Obese and Hypertensive Population Drives the Global Cholesterol Testing Market, According to New Report by Global Industry Analysts, Inc. 4ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2KemPharm, Inc. Receives Patent from the USPTO for Novel Prodrug of Hydromorphone, KP511 2
... ... ... , , , ... ...
... ... web to cost effectively boost the pool of eligible patients for screening for any ... upload recruitment material approved by their Institutional Review Board (IRB), on their own specific ... ...
... ... ... ... ...
Cached Biology Technology:Spectral granted IDE approval by U.S. FDA to conduct pivotal trial for Toraymyxin(TM) 2Spectral granted IDE approval by U.S. FDA to conduct pivotal trial for Toraymyxin(TM) 3Spectral granted IDE approval by U.S. FDA to conduct pivotal trial for Toraymyxin(TM) 4RESolutions Rapid Enrollment Solutions, LLC Announces Launch of Novel Patient Recruitment Web Site - Clinical Trial Spotlight™ 2RESolutions Rapid Enrollment Solutions, LLC Announces Launch of Novel Patient Recruitment Web Site - Clinical Trial Spotlight™ 3China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 2China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 3China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 4China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 5China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 6China-Biotics, Inc. Inaugurated the Commercial Production of its New Production Facility 7
(Date:9/30/2014)... DURHAM, N.C. -- Pollution in urban and farm runoff ... a new study finds. , The study, published ... shows that nitrogen in the runoff ends up in ... tumors on the animals, eyes, flippers and internal organs. ... and the National Oceanic and Atmospheric Administration (NOAA) conducted ...
(Date:9/30/2014)... wrong with your muscles because of age, disease or injury, ... That,s where a new research report published in the October ... comes in. In the report, a team of scientists produce ... scientists "see" which genes are active in an organ at ... and that men have approximately 400 more active genes in ...
(Date:9/30/2014)... , Sept. 30, 2014  Spectra ... solutions for the biotech and power generation ... new RECONN Bioprocess Manager, an easy-to-use and ... geared towards bioprocess development laboratories. ... disparate bioreactor and analyzer systems. Accessing and ...
Breaking Biology News(10 mins):Pollution linked to lethal sea turtle tumors 2Pollution linked to lethal sea turtle tumors 3Scientists identify which genes are active in muscles of men and women 2Spectra Automation Introduces RECONN Bioprocess Manager 2
... National Institute of Standards and Technology (NIST) have ... clumps of proteins in protein-based pharmaceuticals. This first ... can be assured that their instruments are faithfully ... concern because of its impact on quality control ...
... laundry products and air fresheners found the products emitted ... off at least one chemical regulated as toxic or ... was listed on the product labels. , "I first ... me that the air fresheners in public restrooms and ...
... the first issue of , Energy & Environmental Science ... Free for all to read online, this inaugural ... hydrogen storage, thermochemical biofuel production, sustainable energy, and much ... of the highest quality, knowing that they can rely ...
Cached Biology News:NIST trumps the clumps: Making biologic drugs safer 2Toxic chemicals found in common scented laundry products, air fresheners 2Toxic chemicals found in common scented laundry products, air fresheners 3
...
...
... and supplies, for 10 chips, provides ... perform standard-sensitivity RNA analysis (nanogram levels) ... automated electrophoresis system. Supplied are 1,250 ... StdSens stain, 20 microliters RNA ladder, ...
... vectors are designed for enhanced translation of cloned ... rabbit reticulocyte lysate. The maps for pCITE-2b(+) and ... following exceptions: pCITE-2b(+) is a 3793 bp plasmid; ... and Xcm I sites. Subtract 7 bp from ...
Biology Products: