Navigation Links
Enzyme Immunoassay for Studying Intracellular Levels of cAMP

emoved by a wash step. The reaction is completed by the addition of the colorimetric substrate, 3,3,5,5-tetramethylbenzidine/hydrogen peroxide (TMB/H2O2). The addition of TMB/H2O2 to each well results in a blue-green product background, and the reaction is stopped by the addition of 1 M phosphoric acid. A bright yellow color results, and absorbance of peroxidase-labeled cAMP that is bound to the cAMP-specific antibody is determined using a spectrophoto- metric plate reader at 450 nm. Since the amount of cAMP-horseradish peroxidase bound to rabbit anti-cAMP antibody is inversely proportional to cAMP in the sample (or standard), a high absorbance value corresponds to low levels of cAMP present in the sample. The EIA cAMP Kit includes cAMP standards for calculating standard curves. As an option for increased sensitivity (figure 2), standards and prepared samples can be acetylated by treatment with acetic anhydride in the presence of triethylamine.1 Picomolar detection is possible for acetylated cAMP, whereas nonacetylated cAMP levels can generally be assayed in the nanomolar range. The EIA cAMP Kit includes all the re- agents and protocols for quantifying cAMP levels in both nonacetylated and acetylated preparations.

AC Toxin for Increased Intracellular cAMP Levels

Stratagene is offering adenylate cyclase (AC) toxin, a useful reagent for increasing intracellular cAMP levels in cultured cells. AC toxin can be used to study cAMP-dependent protein kinase activation, the key regulatory event in signal transduction pathways involving cAMP. Modulators currently available for examining the effects of cAMP on intracellular signaling include
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Greater Amplification Specificity with New Hot Start PCR Enzyme
5. Improve Amplification Specificity with Hot Start PCR Enzyme
6. Choice of RT-PCR Enzymes
7. Properties of PCR Enzymes
8. Select the Optimal Enzyme for RT-PCR
9. Enzyme Purification With the Econo-Pac Q Cartridge
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Enzyme Immunoassay for Studying Intracellular Levels cAMP

(Date:7/31/2014)... , July 31, 2014 According ... Research "Electrodes for Medical Devices Market (Diagnostic Electrodes - ... Electrodes - TENS, Pacemakers, Defibrillators, Electrosurgical and Other Electrodes) ... Forecast, 2013 - 2019," the global electrodes market for ... 2012 and is estimated to reach a market worth ...
(Date:7/31/2014)... DIEGO and SHENZHEN, China ... announced today the purchase of an Irys™System ... organization. BGI selected BioNano,s platform to enable comprehensive exploration ... improved assemblies for various organisms of interest, including those ... will partner together to develop new methods for multiplexing ...
(Date:7/31/2014)... IL (PRWEB) July 31, 2014 ... of automation across the U.S., increasing unemployment, depressing ... class. It is a Catch 22—we need ... but this means fewer jobs,” says economist and ... just-released Pollina Corporate Top 10 Pro-Business States for ...
(Date:7/31/2014)... 2014 Sales Horizons, a leader in ... sales training course – Mastering the Complex Sale ... , Drawn from proven sales training programs used by ... Complex Sale online sales training course is available to ... face-to-face contact with customers, like technical support, engineers, clinical ...
Breaking Biology Technology:Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 2Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 3Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 4Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 5Electrodes for Medical Devices Market Expected to Reach USD 1.45 Billion Globally in 2019: Transparency Market Research 6BioNano Genomics Announces the Purchase of the Irys System by BGI 2BioNano Genomics Announces the Purchase of the Irys System by BGI 3BioNano Genomics Announces the Purchase of the Irys System by BGI 4ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 2ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 3ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 4Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 2Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 3
... SynCardia Systems, Inc. ( www.syncardia.com ), manufacturer of ... today that Texas Children,s Hospital in Houston has discharged ... for a matching donor heart at home. On Aug. ... Freedom® portable driver, a wearable power supply for his ...
... 7, 2011 Eppendorf AG has licensed ... Bio Inc. for use in their thermal cyclers, including ... technology in its Eppendorf Mastercycler® pro gradient, Mastercycler gradient ... based in Hamburg, Germany, develops, produces and distributes instruments ...
... 16 Nanotechnology products and processes were unveiled at ... mark the fifty-second anniversary celebrations of the Asian ... Center of Excellence in Nanotechnology (CoEN) at AIT, ... and processes demonstrates potential development of Thailand as ...
Cached Biology Technology:1st Pediatric Total Artificial Heart Patient Discharged Home Using Freedom® Portable Driver 21st Pediatric Total Artificial Heart Patient Discharged Home Using Freedom® Portable Driver 3AIT unveils portfolio of 16 nanotechnology products 2
(Date:7/30/2014)... A deep-sea octopus protected and tended her eggs until ... published July 30, 2014 in the open-access journal ... Aquarium Research Institute and colleagues. , Octopuses typically have ... females protect and take care of their fertilized eggs ... eggs for 1 to 3 months, but little is ...
(Date:7/30/2014)... the shortest distance between two points is a straight ... have been taking the familiar axiom to heart. ... to be intense, with each racing for the chance ... species, sperm form cooperative groups that allow them to ... A new study, conducted by Heidi Fisher, a post-doctoral ...
(Date:7/30/2014)... joint venture between The University of Texas at San ... Center at San Antonio, has selected the winners of ... from UTSA and Lei Huang from the Health Science ... engineered ferritins for tracking and protecting neural stem cells ... focused on finding a treatment for stroke through stem ...
Breaking Biology News(10 mins):Deep-sea octopus has longest-known egg-brooding period 2When cooperation counts 2When cooperation counts 3
... NANO -- World,s smallest antenna . . . ... metal or dipole antenna, electronic devices of tomorrow could incorporate ... possible by a design that allows an electrically charged nano-mechanical ... be the days when we need to match the antenna ...
... the day a machine can draw your blood, screen it ... and pop out a drug tailor-made for your DNA. ... point irregularities which have accumulated over time that can lead ... National Institutes of Health has tapped Viterbi professor Andrea Armani ...
... Finally, a gazelle comes into view. The cheetah plunges forward, ... the hapless gazelle by the jugular in less than a ... A blue whale surfaces, blasting water high from its ... with air. Then it descends again to look for krill, ...
Cached Biology News:Story tips from the US Department of Energy's Oak Ridge National Laboratory October 2010 2Story tips from the US Department of Energy's Oak Ridge National Laboratory October 2010 3Fighting cancers by tagging their triggers 2Slicing proteins with Occam's Razor 2Slicing proteins with Occam's Razor 3Slicing proteins with Occam's Razor 4
... aa 16-25 of human GSK3 alpha. ... Store vial at -20 C prior to opening. Dilute ... and freeze at -20 C or below. Avoid cycles ... Sub-Family: not assigned-Other ...
... ligase (AMP forming), Acetate thiokinase ... Physical form: Lyophilized powder containing ... glutathione Preparation Unit Definition: One ... coenzyme A from acetate, ATP and coenzyme ...
...
... PGF2α is a metabolically stable ... a potent uterine stimulant and ... intramuscularly to induce labor. It ... progesterone concentrations when given as ...
Biology Products: