Navigation Links
Enzyme Immunoassay for Studying Intracellular Levels of cAMP

cause cAMP can activate a cascade of reactions, the involvement of cAMP greatly amplifies the response to a hormonal stimulus.

Nonradioactive Detection of cAMP

figure 1

Since changes in cAMP levels can result in the activation of proteins known to play key roles in signal transduction cascades, quantitative measurment of cAMP can serve to identify signaling events. Popular methods for determining cAMP levels include radioimmunoassays (RIA). Although these assays are highly sensitive, they require radioisotopes and time-consuming protocols. In contrast, Stratagenes nonradioactive EIA cAMP Kit uses a competitive EIA (figure 1) assay for simple and sensitive measurement of intracellualar cAMP levels. The assay is based on the competition between cAMP present in the sample and a fixed quantity of a cAMP-peroxidase conjugate that binds to rabbit anti-cAMP-specific antibodies.

figure 2

The assay is performed in a microtiter plate, with each well precoated with goat anti-rabbit IgG. Rabbit anti-cAMP antibodies are combined with the sample and cAMP-horseradish peroxidase conjugate (tracer) and applied to the microtiter well. Samples containing cAMP compete with a fixed quantity of tracer for the rabbit anti-cAMP-specific antibodies. All unbound reagents are r
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. The Best Enzyme for the Toughest PCR Challenges Improved with New Hot-Start Feature
2. Comparing Fidelity and Performance of Proofreading PCR Enzymes
3. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
4. Greater Amplification Specificity with New Hot Start PCR Enzyme
5. Improve Amplification Specificity with Hot Start PCR Enzyme
6. Choice of RT-PCR Enzymes
7. Properties of PCR Enzymes
8. Select the Optimal Enzyme for RT-PCR
9. Enzyme Purification With the Econo-Pac Q Cartridge
10. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
11. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Enzyme Immunoassay for Studying Intracellular Levels cAMP

(Date:8/21/2014)... team including DESY scientists has observed tiny quantum vortices ... in the journal Science that the exotic ... nanodroplets. It is the first time that the quantum ... of what is known as superfluid helium, have been ... expectations," says Andrey Vilesov of the University of Southern ...
(Date:8/21/2014)... , August 21, 2014 ... ) has announced the addition of the  ... to their offering.       (Logo: ... hormone therapy, is a means of replacing ... by the body. This type of therapy ...
(Date:8/21/2014)... N.Y. , Aug. 21, 2014 ... launch of their Nutraceutical "TELO-20 for Dogs" with ... only Telomere-lengthening supplement in the world for dogs. ... of every chromosome in the body. A wealth ... leading experts in Telomere Science and Aging, including ...
(Date:8/21/2014)...  Decision Resources Group finds that the asthma ... Mexico currently faces severe ... agents, the anticipated label extension of Boehringer Ingelheim/Pfizer,s ... emerging biologics will substantially increase the competitiveness of ... inhibitors will introduce a new dynamic to the severe, ...
Breaking Biology Technology:Scientists observe quantum vortices in cold helium droplets 2Global Hormone Replacement Therapy Market 2014-2018: Key Vendors are Actavis, Bayer, Merck & Co, Novartis and Novo Nordisk 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 3Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3
... 26 Verenium Corporation (Nasdaq: VRNM ), ... enzyme solutions, today announced that it has renewed and ... leading global provider of heat transfer, separation and fluid ... Alfa Laval signed an agreement to jointly market enzymatic ...
... Pa. and LEUSDEN, Netherlands, Oct. 26 Molecular ... designed to increase the speed and accuracy of ... distributor Westburg BV today announced that they have ... MDI,s Detect-Ready MRSA panel in the Benelux region. ...
... 26 iDiverse announced that it has successfully modified ... lethal stresses normally encountered in the bioproduction of fuel ... to generate significantly more ethanol. "We ... and are rapidly developing a 2nd generation with yet ...
Cached Biology Technology:Verenium and Alfa Laval Announce Extension of Purifine® PLC Enzyme Agreement 2Verenium and Alfa Laval Announce Extension of Purifine® PLC Enzyme Agreement 3Verenium and Alfa Laval Announce Extension of Purifine® PLC Enzyme Agreement 4Molecular Detection Inc. Announces MSSA Label Expansion and Benelux Distribution Agreement for Its Detect-Ready™ MRSA Screening Test 2Molecular Detection Inc. Announces MSSA Label Expansion and Benelux Distribution Agreement for Its Detect-Ready™ MRSA Screening Test 3Molecular Detection Inc. Announces MSSA Label Expansion and Benelux Distribution Agreement for Its Detect-Ready™ MRSA Screening Test 4Molecular Detection Inc. Announces MSSA Label Expansion and Benelux Distribution Agreement for Its Detect-Ready™ MRSA Screening Test 5iDiverse Develops Stress Resistant Yeast for Producing Fuel Ethanol 2
(Date:8/21/2014)... study of American singles found that during sex ... orgasm rates. On average, men experience orgasm 85.1 ... making little difference. For women, however, orgasm occurrence ... 62.9 percent of the time during sex with ... with women,s sexual orientation, with lesbian women experiencing ...
(Date:8/21/2014)... linked to dandruff, eczema and other itchy, flaky maladies ... global reachesincluding Hawaiian coral reefs and the extreme environments ... review in the scientific journal PLOS Pathogens ... of the genus Malassezia in light of ... around the world. , University of Hawai,i at Mānoa ...
(Date:8/21/2014)... In a finding that has implications for life in ... in the solar system, LSU Associate Professor of Biological ... National Science Foundation, or NSF, this week published a ... lake that lies 800 meters (2600 feet) beneath the ... microbial ecosystems.", Given that more than 400 subglacial lakes ...
Breaking Biology News(10 mins):Orgasm rates for single women less predictable than men's, vary by sexual orientation 2Orgasm rates for single women less predictable than men's, vary by sexual orientation 3Orgasm rates for single women less predictable than men's, vary by sexual orientation 4From dandruff to deep sea vents, an ecologically hyper-diverse fungus 2800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 2800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 3800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 4
... Philadelphia, PA, March 1, 2012 Down syndrome ... born children arising as a consequence of a chromosomal ... copies of chromosome 21, instead of the usual two. ... cognitive dysfunction that can range from mild to severe ...
... is the largest sea turtle in the world, growing ... also one of the most threatened. A major new ... zonesin the Pacific Ocean for this critically endangered species. ... practices to help reduce further deaths of this fragile ...
... A*STAR scientists have for the first time, identified that ... stem cell (ESC) self-renewal the ability of ESCs to ... for better understanding of ESC regulation and was published in ... by the team of scientists from the Institute of Medical ...
Cached Biology News:Understanding and treating the cognitive dysfunction of Down syndrome and Alzheimer's disease 2Leatherback turtle migration study identifies Pacific danger zones for endangered species 2A*STAR scientists make groundbreaking discovery on stem cell regulation 2
... The ImageXpress 5000A automated cellular imaging and ... software system designed specifically for rapid acquisition ... throughput cell-based screening. ImageXpress is fast. ... resolution (1280 x 1024 pixels), with two ...
...
... Custom Peptide Services offer a full range ... research needs. Services include: Peptide synthesis, ... Peptide synthesis, purified product (70-95% pure) ... amidation, biotinylation, fluorescein, myristylation, phosphorylation, and rhodamine) ...
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. Material: ...
Biology Products: