( ...)Enjoy a Greater Sense of Security,,,with PCR-Grade Water

Like all components of a PCR or RT-PCR assay, the water component must be of high purity. Standard laboratory water is often contaminated with DNA, RNA, nucleases, or a variety of microorganisms. These contaminants often contribute to suboptimal amplification reactions.

Roche Applied Sciences LightCycler Kits and other amplification reagents contain specially purified, deionized, double-distilled, and autoclaved water. The unique production process also ensures that no detectable amounts of nucleic acids are included. This is routinely confirmed with conventional thermal cyclers and in highly sensitive assays with the LightCycler Instrument and related LightCycler Kits.

In addition, this water is guaranteed to be free of detectable endonucleases, ribonucleases, and nicking activity.

With all the things that can go wrong in a PCR or RT-PCR reaction, give yourself a greater sense of security by choosing PCR-grade water from Roche Applied Science now available as a single reagent.

Rapid Protein Engineering: Expression of Truncation Mutants with the RTS 100 System

Now that the post-genomic era has begun, rapid protein expression is one of the major tasks to be addressed by the molecular biology field. In order to assign functions to the genes encoded in the enormous amounts of sequence data, mutagenesis methods are of paramount importance. By combining a powerful polymerase chain reaction (PCR) technique to generate linear expression fragments with a rapid in vitro protein expression method, a new tool for protein engineering has been created.




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