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Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep ,,, Technology

ime period. Without Ion Sweep gas, the caffeine signal is lost in the base peak chromatogram, as observed in Figure 3a. The signal due to caffeine (m/z 195) is overwhelmed by signal due to solvent clusters at m/z 150 and m/z 160, as evident from the mass spectrum (Figure 3b). Figure 3: Flow injection analysis of caffeine without use of Ion Sweep gas. The presence of solvent adduct ions (m/z 150 and m/z 160) overwhelm the caffeine signal at m/z 195 in both a) the base peak chromatogram and b) mass spectrum. When Ion Sweep gas is used, the background noise is drastically reduced and each injection is clearly visible in the base peak chromatogram, as illustrated in Figure 4a. Furthermore, m/z 195 is the base peak in the mass spectrum (Figure 4b). Figure 4: Flow injection analysis of caffeine using Ion Sweep gas at a setting of 10 arbitrary units. Reducing the background solvent adduct ions yields significantly greater analyte signal-to-noise in the base peak chromatogram (a). The mass spectrum (b) also shows a base peak of the caffeine (m/z 195) and no significant noise due to solvent adduct ions at m/z 150 and m/z 160, as seen in Figure 3b. Similar improvements are observed in analysis of buspirone (m/z 386.4), as illustrated in Figures 5 and 6. Again, the solvent ion clusters at m/z 150 and m/z 160 elevate the background in the base peak ion trace, as well as the mass spectrum, when the Ion Sweep gas is turned off (see Figure 5). The remarkable increase in S/N when the Ion Sweep gas is turned on at 10 arbitrary units (au), as presented in Figure 6, illustrates the
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