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Enhancing PCR* Efficiency with MasterTaq

Ruler DNA ladder (MBI Fermentas)
2: display RT/hsp60
3: MMuLV RT/hsp60
4: display RT/hsp27
5: MMuLV RT/hsp27
A: Eppendorf MasterTaq 1u/50 l
B: MBI Fermentas TAQ 1 u/50 l



Figure 2: Northern blot of LT23 human lung adenocarcinoma cell RNA (8 g) hybridized with hsp60 and hsp27 probe, respectively (protocol available from the authors). Image derived from Phosphorimager after 8 h exposure.

These results demonstrate the positive effect of TaqMaster on thermal stability and processivity of Taq DNA Polymerase. The use of this PCR enhancer dramatically improves RT-PCR sensitivity and allows the amplification of target genes that otherwise prove difficult or impossible to be detected via RT-PCR.

References:

  1. Liang P and Pardee AB. Differential display of eukaryotic messenger RNA by means of polymerase chain reaction. Science. 1992:257(5072);967-71.
  2. Sagerstrm CG, Sun BI, Sive HL. Subtractive cloning: past, present, and future. Annu Rev Biochem. 1997;66:751-83.
    Soares MB. Identification and cloning of differentially expressed genes.
  3. Curr Opin Biotechnol. 1997;8(5):542-6.
  4. Wang Z and Brown DD. A gene expression screen. Proc Natl Acad Sci. 1991;88:11505-9.


Fermentas is a registered trademark of AB Fermentas CORPORATION.
This product is sold under licensing arrangements with F. Hoffman-La Roche Ltd., Roche Molecular Systems, Inc. and Applied Biosystems.
*Practice of the patented polymerase chain reaction (PCR) process requires a license.
**The Eppendorf Thermal Cycler is an Auth
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