| HOME >> BIOLOGY >> TECHNOLOGY |
Retroviral-mediated Delivery of siRNA
The pSilencer 5.1 Retro System
enables researchers to study the long-term effects of reducing
the expression of specific genes in cell culture models. These
retroviral vectors make use of H1 or U6 Polymerase III
promoters to stably express siRNAs, even in
difficult-to-transfect cell types. This technology expands RNA
interference research to address long-term alteration of the
expression of genes that cause disease phenotypes.
Introduction: Getting siRNAs into Cells
with Viruses
The use of siRNAs
to induce gene silencing has enormous potential for
identifying and understanding gene function and may eventually
be used as a therapeutic strategy. Approaches to produce siRNA
molecules include chemical synthesis, enzymatic synthesis, and
DNA-based expression vectors. In general, production of siRNA
in vitro by chemical synthesis is preferred for most transient
experiments. However, DNA-based approaches have distinct
advantages when performing long-term RNAi studies or when
conducting studies in cells or organisms into which siRNAs are
particularly difficult to deliver.
The DNA-mediated siRNA expression techniques
can be divided into viral and nonviral methods. Both involve
the expression of a short hairpin RNA transcript that is
processed endogenously to an siRNA. Although the nonviral
mediated approach is effective for transient RNA interference
'"/>
Source:
Related biology technology :
1. Enhanced PCR Cloning System
2. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
3. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
4. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
5. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
6. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
7. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Custom and library siRNA for efficient gene silencing
10. Custom and library siRNA for efficient gene silencing
11. Cancer siRNA Oligo Set Version 1.0
| Copyright © 2003-2012 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |