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Enhanced PCR Cloning System

ed the kits to include the StrataPrep PCR purification kit and XL10-Gold ultracompetent cells. The StrataPrep kit allows PCR products to be rapidly and efficiently purified from excess primers. XL10-Gold Kan ultracompetent cells provide the highest transformation efficiency of ligated DNA and easy-to-score colonies. Finally, these improvements were added to the PCR-Script kit without increasing the price of the kit.

REFERENCES

  1. Bauer, J., et al. (1992) Strategies 5: 62-65.

  2. Pearson, S. and Bauer, J.C. (1996) Strategies 9: 26-27.

  3. Sanchez, T., Zheng, C.-F., and Bauer, J. (1996) Strategies 9: 44-46.

  4. Scott, B., et al. (1994) Strategies 7: 6263.

  5. Hogrefe, H., et al (1997) Strategies 10: 93-96.

  6. Braman, J. and Basehore, S. (1997) Strategies 10: 84-86.

  7. Jerpseth, B., Callahan, M., and Greener, A. (1997) Strategies 10: 37-38.

* U.S. Patent Nos. 5,512,468 and 5,707,841 and patents pending.
** U.S. Patent No. 5,545,552 and patents pending.


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