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Enhanced Amplification of Long Targets with PfuTurbo DNA,,,Polymerase

d 6). Increased performance in 1.5X buffer was partly, but not entirely, due to the 50% increase in MgSO4 concentration (data not shown). PCR buffer titration experiments, carried out with additional PCR systems, indicate that a PCR buffer concentration of 1.5X is consistently optimal for long PCRs with PfuTurbo DNA polymerase.5

The error rate of PfuTurbo DNA polymerase in 1.5X PCR buffer was measured as described.5 No significant differences in error rates were observed when PCRs were performed in 1.5X PCR reaction buffer, compared to the 1X concentration (Table 1).

Table 1
PCR Enzyme Error Rates

The error rate of PfuTurbo DNA polymerase in 1.5X PCR buffer was measured as described.5 No significant difference in error rates were observed when PCRs were performed in PCR reaction buffer at 1.5X, compared to the 1X concentration.

PCR enzyme

PCR reaction buffer

Error rate (x10-6)
(mean variation)

PfuTurbo DNA Polymerase

1X cloned Pfu buffer

1.3 0.2

PfuTurbo DNA Polymerase

1.5X cloned Pfu buffer

1.1 0.1

Taq DNA Polymerase

1X Taq buffer

11
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