Navigation Links
Enhanced Amplification of Long Targets with PfuTurbo DNA,,,Polymerase

d 6). Increased performance in 1.5X buffer was partly, but not entirely, due to the 50% increase in MgSO4 concentration (data not shown). PCR buffer titration experiments, carried out with additional PCR systems, indicate that a PCR buffer concentration of 1.5X is consistently optimal for long PCRs with PfuTurbo DNA polymerase.5

The error rate of PfuTurbo DNA polymerase in 1.5X PCR buffer was measured as described.5 No significant differences in error rates were observed when PCRs were performed in 1.5X PCR reaction buffer, compared to the 1X concentration (Table 1).

Table 1
PCR Enzyme Error Rates

The error rate of PfuTurbo DNA polymerase in 1.5X PCR buffer was measured as described.5 No significant difference in error rates were observed when PCRs were performed in PCR reaction buffer at 1.5X, compared to the 1X concentration.

PCR enzyme

PCR reaction buffer

Error rate (x10-6)
(mean variation)

PfuTurbo DNA Polymerase

1X cloned Pfu buffer

1.3 0.2

PfuTurbo DNA Polymerase

1.5X cloned Pfu buffer

1.1 0.1

Taq DNA Polymerase

1X Taq buffer

11
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Enhanced PCR Cloning System
2. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
3. Enhanced siRNA Delivery and Long-term Gene Silencing
4. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
5. Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
6. Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
7. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
10. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
11. Greater Amplification Specificity with New Hot Start PCR Enzyme
Post Your Comments:
(Date:9/18/2014)... Woburn, MA (PRWEB) September 18, 2014 ... Single Molecule Sequencing (tSMS™) technology, announced today the ... round of investment, led by Genomic Diagnostic Technologies, ... scientific and diagnostic applications. , Founded in March ... Scientist and Bioinformatics Analyst at Helicos BioSciences, SeqLL ...
(Date:9/18/2014)... to weight loss and diabetes prevention, could actually hasten ... they do it in a surprising way: by changing ... the substantial population of bacteria residing in our intestines. ... humans, were published today in Nature . Among ... Institute,s Immunology Department, who led this research together with ...
(Date:9/18/2014)... quest for the ultimate memory device for computing may ... The City College of New York led by chemist ... both magnetic and ferroelectric properties. , Combining both properties ... occur between them and for the devices that might ... these two properties in a single material, however, has ...
(Date:9/18/2014)... MX (PRWEB) September 18, 2014 September ... Mexico City, MX. ASEA is the manufacturer of the ... the skin care product, RENU28. , The Mexico ... Vice President Duvan Botero, and ASEA Field Leader Shawn ... holds a PhD in Atomic/Medical Physics from the University ...
Breaking Biology Technology:SeqLL Closes Series-A Funding Round for Expansion of Single Molecule Sequencing 2Gut bacteria, artificial sweeteners and glucose intolerance 2Gut bacteria, artificial sweeteners and glucose intolerance 3Oxides discovered by CCNY team could advance memory devices 2Announcing the ASEA Mexico Grand Opening Tour Beginning September 18th 2
... Keck Graduate Institute,(KGI) today announced a partnership with ... forum to help women managers advance in the,life ... forum from Jan. 13-18, 2008. Attendees will,have the ... executives from,pharmaceutical, medical device and biotechnology companies along ...
... 21 Nile Therapeutics Inc., (OTC,Bulletin Board: SPDU) ... disease, is pleased to announce the,appointments of Daron ... as the Vice President of Development. Mr. ... Chief Operating Officer,and was appointed Chief Financial Officer ...
... of Physics Dr. Satyendra Kumar recently was honored ... Directors Award for Excellence for recognition of exceptional ... matter physics proposals. In all, seven program directors ... Arlington, Va. , The NSF Directors Award ...
Cached Biology Technology:KGI and Smith College Establish Leadership Forum for Women in Bioscience 2Nile Therapeutics, Inc. Adds to Executive Management Team 2Nile Therapeutics, Inc. Adds to Executive Management Team 3Nile Therapeutics, Inc. Adds to Executive Management Team 4
(Date:9/18/2014)... 2014 -- Arizona turned its newborn screening program into a ... months, earning it the first-ever Newborn Screening Quality Award from ... presented Will Humble, M.P.H., Arizona,s Department of Health Services director, ... Humble established a policy of full transparency for the length ... samples to the lab for analysis, and set a target ...
(Date:9/18/2014)... which have devastated algal forests in the eastern Mediterranean ... basin if their distribution continues to expand as the ... an international team of researchers led by Dr Adriana ... the Mediterranean Institute for Advanced Studies in Spain, is ... of the team surveyed more than 1000 kilometres of ...
(Date:9/18/2014)... the fish world, are the ideal animal in which ... moved from the ocean into tens of thousands of ... changing their skeleton to adapt to the new environment. ... sticklebacks now have turned up one of the genes ... change in that gene,s regulation in a freshwater population ...
Breaking Biology News(10 mins):March of Dimes honors Arizona with first-ever newborn screening award 2March of Dimes honors Arizona with first-ever newborn screening award 3Tropical fish a threat to Mediterranean Sea ecosystems 2Counting fish teeth reveals regulatory DNA changes behind rapid evolution, adaptation 2Counting fish teeth reveals regulatory DNA changes behind rapid evolution, adaptation 3
... When the larvae of the primitive social insect Polistes ... she doesnt know if shell arise a worker or gyne ... social insect researcher Gro Amdam. Amdams group is ... solitary ancestors through study of a primitive social order of ...
... Vitamin D deficiency early in pregnancy is associated with ... study from the University of Pittsburgh Schools of the ... Clinical Endocrinology and Metabolism. A serious complication of ... the hands and feet, preeclampsia is the leading cause ...
... researchers at Boston Universitys College of Engineering may ... capable of defeating so-called superbugs, bacteria that have ... discovered a previously unknown chain of events occurring ... avenues of research. Currently, three classes ...
Cached Biology News:Old developmental pathways spawn revolutionary evolutionary changes 2Old developmental pathways spawn revolutionary evolutionary changes 3Low vitamin D during pregnancy linked to pre-eclampsia 2Boston University biomedical engineers find chink in bacteria's armor 2Boston University biomedical engineers find chink in bacteria's armor 3
... Detection Systems allow non-radioactive detection of ... systems, biotinylated lysine residues are incorporated ... the need for labeling with [35-S]methionine ... biotinylated lysine is added to the ...
... Series Hybridization chambers with forced convection ... ambient up to 99.9 C / 212 F ... and reproducible results DS controller Timer ... with an accuracy of a tenth of a ...
... of your microarray data using Asuragens bioinformatics ... from consultation to extensive data analysis using ... our expertise in combination with the Gene ... microarray experiments., ,Normalization and basic statistical analysis ...
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. Material: cap ...
Biology Products: