( d 6). Increased performance in 1.5X buf...)Enhanced Amplification of Long Targets with PfuTurbo DNA,,,Polymerase

d 6). Increased performance in 1.5X buffer was partly, but not entirely, due to the 50% increase in MgSO₄ concentration (data not shown). PCR buffer titration experiments, carried out with additional PCR systems, indicate that a PCR buffer concentration of 1.5X is consistently optimal for long PCRs with PfuTurbo DNA polymerase.⁵

The error rate of PfuTurbo DNA polymerase in 1.5X PCR buffer was measured as described.⁵ No significant differences in error rates were observed when PCRs were performed in 1.5X PCR reaction buffer, compared to the 1X concentration (Table 1).

Table 1
PCR Enzyme Error Rates

The error rate of PfuTurbo DNA polymerase in 1.5X PCR buffer was measured as described.⁵ No significant difference in error rates were observed when PCRs were performed in PCR reaction buffer at 1.5X, compared to the 1X concentration.

PCR enzyme

PCR reaction buffer

Error rate (x10^-6)
(mean variation)

PfuTurbo DNA Polymerase

1X cloned Pfu buffer

1.3 0.2

PfuTurbo DNA Polymerase

1.5X cloned Pfu buffer

1.1 0.1

Taq DNA Polymerase

1X Taq buffer

11
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