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EndoFree Plasmid Kits

Plasmid Purification Method vs. Transfection Efficiency Two independent pRSVcat preparations obtained with each method shown were each transfected twice into COS-7, HeLa, and LMH cells using a liposome-mediated method and into Huh7 cells using calcium phosphate. Average transfection efficiencies are expressed as percentages relative to the efficiency obtained with DNA prepared using the QIAGEN Plasmid Kit (100%). Plasmid Purity vs. Transfection Efficiency Effect of the amount and quality of plasmid DNA on transfection efficiency in CHO SSF3 X9 cells grown in suspension under serum-free conditions. Each point represents the mean of three independent experiments; Rel. LU: relative light units. Data kindly provided by M. Zang-Gandor, Novartis AG, Basel, Switzerland.

* For purification of low-copy plasmids and cosmids, EndoFree Plasmid Mega Kits are a better choice than EndoFree Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.



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Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
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3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
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7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
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11. RNA-free Plasmid DNA Isolation Protocol
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