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EndoFree Plasmid Kits

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EndoFree Plasmid Kits yield endotoxin-free DNA which is ideal for high reproducibility and efficiency in transfection (see figures "Plasmid Purification Method vs. Transfection Efficiency" and "Plasmid Purity vs. Transfection Efficiency" and tables "Endotoxin levels in plasmid preparations" and "EndoFree DNA yields high transfection efficiencies with primary cells"). QIAGEN ultrapure endotoxin-free DNA is also suitable for gene therapy research and genetic vaccination and other sensitive applications.

Endotoxin levels in plasmid preparations*



*Host strain: DH5 a plasmid: pRSVcat
1 ng LPS = 1.8 EU
Calculated from data in Figure 9.19

EndoFree DNA yields high transfection efficiencies with primary cells Primary rabbit gastric parietal cells were transfected with pEGFP-N2 (CLONTECH) prepared by the methods indicated. Transfections were performed using Effectene Transfection Reagent. The data represent the percentage of cells expressing GFP as determined by scoring the number of green fluorescent cells 48 h post transfection. The transfection efficiencies represent the average from 6 to 9 replicate dishes from more than 2 different DNA preps for each purification method. (Data kindly provided by C. Chew and J. Parente, Department of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, USA.)
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