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EndoFree Plasmid Kits

For purification of endotoxin-free ultrapure plasmid or cosmid DNA

Features and benefits
  • Less than 0.1 EU/g plasmid DNA
  • Yields of up to 10 mg plasmid DNA
  • Greater than 90% recovery of plasmid DNA
  • Fast and simple procedure



* Actual yields depend on plasmid copy number, size of insert, host strain, culture medium, and culture volume.
Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.
For purification of low-copy plasmids and cosmids, EndoFree Plasmid Mega Kits are a better choice than EndoFree Plasmid Giga Kits, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge

Background

Endotoxins, also known as lipopolysaccharides or LPS, are cell-membrane components of Gram-negative bacteria such as E. coli (see figure "Schematic diagram of the envelope of E. coli."). Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines (see figures "Plasmid Purification Method vs. Transfection Efficiency"and "Plasmid Purity vs. Transfection Efficiency" and tables "Endotoxin levels in plasmid preparations" and "EndoFree DNA yields high transfection efficiencies with primary cells"). Furthermore, endotoxins can influence the uptake of plasmid DNA in
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