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Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

ratory band 11, which is a doublet, is partially resolved in UCD 522A. The laboratory form of band 12 (chromosome IV) is not present. Instead, a faster- migrating band is seen which just merges with the partially resolved doublet corresponding to laboratory band 11. The karyotype of the genetically purified and presumably homozygous clone 522XA (lane D) has a different profile than its progenitor, UCD 522A. The band migrating just ahead of laboratory band 3 is absent while laboratory doublet band 11, partially resolved in UCD 522A, is completely resolved.

The profile of UCD 505K (lane E) indicated the presence of laboratory band 3, the faster migrating band seen in the UCD 522A profile, and a unique band migrating between laboratory bands 4 and 5. The faster migrating form of laboratory band 7 seen in UCD 522A and 522XA has a diffuse counterpart in UCD 505K profile. Laboratory bands 10, 11, and 13 have approximate counterparts in UCD 505K, but band 12 is absent.

The profile of UCD 595A (lane F) indicated a very faint counterpart to laboratory band 1, perhaps three merged bands migrating between laboratory bands 2 and 3, and a partially resolved doublet corresponding to laboratory band 5. Laboratory bands 6 through 9 have diffuse and approximate counterparts in this wine strain which may indicate the presence of additional and unresolved chromosomes. As in UCD 505K, laboratory bands 10, 11, and 13 have counterparts in this strain, while band 12 is absent. Instead, a faint band migrates slightly more slowly than the UCD 595A form of laboratory band 11.

While the electrophoretic profiles of UCD 522A, UCD 505K, and UCD 595A provide qualitative evidence for aneuploidy, a more useful qu
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