Navigation Links
Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

olume brought to 10 ml in SE, and the beads incubated at 37 C for 2 to 24 hours. Following incubation, the beads were pelleted as before, resuspended in 20 ml of 25 mM Na2EDTA pH 8.0, containing 1% sarkosyl (Na salt of N-lauroylsarcosine, Sigma) and 50 g/ml proteinase K (Boehringer Mannheim), and incubated overnight at 50 C. The next day they were centrifuged and washed several times in 20 ml aliquots of 50 to 150 mM EDTA, pH 7.5 to 8.0, before being stored as a slurry (approximately 10 ml) in the EDTA solution at 4 C for as long as 2 years.

The following substitutions9 have since produced identical results: SDS instead of sarkosyl; 1 mg/ml pronase E (Sigma P 6911) at 37 C for 2 to 24 hours instead of 50 g/ml proteinase K overnight at 50 C; stationary instead of log phase cultures; use of cells from individual colonies from a YEPD plate (0.25 ml agarose per colony); and a scaled-down but analogous cell work-up with microfuge tubes and 5-second microcentrifuge spins instead of 50 ml tubes and 5-minute low-speed spins.


Results
Electrophoretic Karyotype of the Strains
The karyotype (Figure 1) of the laboratory strains (lanes A, B, G, and H) is virtually identical to that previously described3, and hence the identities of the bands are also presumed to be the same. In the profile of the UCD 522A (lane C), band 1 migrates faster than the laboratory counterpart and although band 4 corresponds well to band 3 (chromosome III) in the laboratory strains, an additional faster migrating band is also present. The doublet corresponding to band 5 in the laboratory strains migrates faster, as does the UCD 522A version of laboratory band 7. Labo
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
2. Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach
3. Saccharomyces cerevisiae
4. Saccharomyces cerevisiae
5. Saccharomyces cerevisiae
6. Saccharomyces cerevisiae
7. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
8. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
Post Your Comments:
(Date:5/5/2015)... 2015 CytomX, a biotechnology company developing Probody™ ... Sean McCarthy , D. Phil., chief executive officer, ... New York at 1:15 p.m. ... an overview of CytomX,s Probody pipeline, including the company,s ... About CytomX Therapeutics CytomX Therapeutics develops Probody™ ...
(Date:5/5/2015)... 05, 2015 Exotic Automation ... announces the launch of http://www.cng.exoticautomation.com , an ... a convenient way to purchase genuine OEM replacement ... , The new website provides owners of ... direct access to high performance products for their ...
(Date:5/5/2015)... CA (PRWEB) May 05, 2015 Synedgen ... Nguyen, PhD as Regulatory Affairs Manager. , Dr. Nguyen ... an important juncture for the company as it moves ... drug products over the next two years. Dr. Nguyen ... of all the regulatory key documents that must be ...
(Date:5/4/2015)... 4, 2015 Tobira Therapeutics, Inc. (NASDAQ: ... development and commercialization of novel treatments for liver ... its merger with Regado Biosciences, Inc. (NASDAQ: RGDO, ... of the merger, Tobira raised $40 million of ... and all current Tobira institutional investors, including the ...
Breaking Biology Technology:Exotic Automation & Supply Launches Ecommerce Website 2Synedgen Announces the Appointment of Regulatory Affairs Manager 2Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 2Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 3Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 4Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 5Tobira Therapeutics Completes Financing and Merger with Regado Biosciences 6
... , , , Multiporator / Electroporator 2510 , , , , , ... Transformation Protocol , Protocol No. 4308 915.534 01/2002 , ... , , , Microorganism , ... Cell type , Bacteria, gram positive, ...
... , , , , , , Multiporator / Electroporator 2510 , , ... , Transformation Protocol , Protocol No. 4308 915.540 ... , , , , , , ... , Cell type , ...
... Electroporator 2510 , , , , , , , , ... Protocol No. 4308 915.522 03/2002 , , , , ... , Microorganism , Mycobacterium smegmatis LR222, ... , Bacteria, gram positive, , ...
Cached Biology Technology:Staphylococcus epidermidis 2Pediococcus spp. 2Mycobacterium smegmatis 2
(Date:4/17/2015)... BURNABY, Canada , April 17, 2015 /PRNewswire/ ... projects, growing security concerns, and technological advancement to ... Arabia until 2020   ... " Saudi Arabia Biometric Systems Market Forecast &  Opportunities, 2020 ", biometric ... is projected to register growth at CAGR ...
(Date:4/10/2015)... , April 10, 2015 ... addition of the "Security Competitive Profiles - NEC" ... http://photos.prnewswire.com/prnh/20130307/600769) , NEC will continue to supply ... to the market, with a company focus on the ... anticipated. Winning opportunities in the ...
(Date:4/2/2015)... 2, 2015 At its 2015 ACMG Annual ... , the American College of Medical Genetics and ... to its Board.  Members of the ACMG Board of ... forming and advancing its policies and programs. ACMG is ... "It,s an eventful time in medical genetics and ...
Breaking Biology News(10 mins):Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3NEC Security Competitive Profile 2015 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 2American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 3American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 4American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 5American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 6American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 7American College of Medical Genetics and Genomics Announces New Board Members: Dr. Louanne Hudgins is ACMG President-Elect 8
... Illinois researchers, Phillip A. Newmark, a professor of cell ... the William H. and Janet Lycan professor of chemistry, ... Newmark and van der Donk are among 56 biomedical ... nationwide competition. The 2008 investigators were selected for their ...
... The University of Nottingham have taken some important first ... cell, a leading science journal reports. Dr Cameron ... School of Pharmacy have used polymers long-chain molecules ... the surfaces of a real cell. In work ...
... summer temperatures could mean some parts of southern England are ... according to a new book launched today (26 May 2008). ... College London, claims that if average summer temperatures in the ... of Hampshire and the Severn valley, which currently contain many ...
Cached Biology News:2 University of Illinois researchers named HHMI investigators 22 University of Illinois researchers named HHMI investigators 3Life, but not as we know it? 2Research suggests parts of UK could be too hot for wine making by 2080 2
... Phosphate Sensor is a highly sensitive ... This product is a purified form of ... the fluorophore MDCC, which is sensitive to ... inorganic phosphate by Phosphate Sensor is rapid ...
... This benchtop ductless exhaust hood ... allowing safe indoor release of exhaust. ... contaminants. Over 28" of vertical clearance ... glassware. Includes polypropylene spill tray; options ...
... cleanroom for biological safety labs (BSL) ... (to Class 10/ISO 3) and easy ... ultra-smooth surfaces that are easy to ... corners simplify cleaning. Unique double-wall panel ...
... modular cleanroom for biological safety labs ... cleanliness (to Class 10/ISO 3) and ... create ultra-smooth surfaces that are easy ... radius corners simplify cleaning. Unique double-wall ...
Biology Products: