Navigation Links
Efficiently Insert Unique Restriction Sites into Plasmid Vectors

d Xho I restriction digestion patterns for seven of the clones are shown in Figure 2, indicating that the appropriate restriction sites were successfully introduced into the plasmid vector.

Fig.2

Conclusions

These results demonstrate that a 28-base insertion, encoding three unique restriction enzyme sites, was effectively introduced into a plasmid vector using the QuikChange mutagenesis kit. Mutagenesis reactions and transformation can be completed in less than one day so putative positive clones may be examined the following day. And, because high-fidelity PfuTurbo DNA polymerase is used, random mutations are minimized, rendering this the preferred method for efficient mutagenesis.

REFERENCES
  1. Papworth, C., Bauer, J.C., and Braman, J. (1996) Strategies 9: 3-4.

  2. Hogrefe, H., et al. (1997) Strategies 10: 93-96.

  3. Braman, J. and Basehore, S. (1997) Strategies 10: 84-86.

* U.S. Patent No. 5,789,166 and patents pending
** U.S. Patent Nos. 5,545,552 and 5,866,395 and patents pending


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
2. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
8. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
9. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
10. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
11. Generate High-Quality, Directional Plasmid cDNA Libraries
Post Your Comments:
(Date:12/19/2014)... Dec. 19, 2014 Naurex Inc., a biopharmaceutical ... for diseases of the central nervous system, today announced ... executive officer, will present at the 33 rd ... take place at 3:00 p.m. PST on Tuesday, January ... San Francisco, Calif. ...
(Date:12/19/2014)... 18, 2014 BioPlus Specialty ... specialty pharmacies, has an expanded team of Regional ... consultants for prescriber offices in their regions, with ... “We have a variety of Regional Pharmaceutical Care ... specializing in hepatitis C help prescribers understand all ...
(Date:12/19/2014)... MA (PRWEB) December 18, 2014 iLab ... announced the appointment of Siri Bryant as the new ... new Director of Core Implementations. These two new leadership ... outstanding customer service by ensuring that iLab continues to ... “In the last four years, iLab has been deployed ...
(Date:12/19/2014)... MILLBROOK, N.Y. , Dec. 18, 2014 Egenix, ... has been elected Chairman of the Board, to succeed ... President and CEO, but will continue as a member of ... that he has added to the company over almost twenty ... The Board of Directors has established a Search ...
Breaking Biology Technology:Naurex to Present at 33rd Annual J.P. Morgan Healthcare Conference 2BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 2BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 3iLab Solutions Announces Two New Leadership Positions to Meet the Needs of Its Growing Customer Base 2
... Calif., July 7, 2011 InterMune (NASDAQ: ... in ASCEND, a new Phase 3 study of pirfenidone ...  ASCEND is a multinational, randomized, double-blind, placebo controlled Phase ... of Esbriet® (pirfenidone) in IPF patients with mild to ...
... America, Inc . ( www.cordblood-america.com ) (OTC Bulletin Board: ... preservation company focused on bringing the life saving potential ... today that CorCell, its domestic brand for cord blood stem ... Rewards Program in the U.S. for the collection of a ...
... a.m. EDT, the space shuttle Atlantis will streak skyward from ... mission. While the STS-135 flight marks the end of the ... new era of research into infectious diseases, thanks to space ... III, along with their colleagues at Arizona State University,s Biodesign ...
Cached Biology Technology:InterMune Initiates Phase 3 ASCEND Study of Pirfenidone in IPF 2InterMune Initiates Phase 3 ASCEND Study of Pirfenidone in IPF 3InterMune Initiates Phase 3 ASCEND Study of Pirfenidone in IPF 4InterMune Initiates Phase 3 ASCEND Study of Pirfenidone in IPF 5CorCell, a Cord Blood America Company, Launches Referral Rewards Program 2Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 2Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 3Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 4
(Date:12/17/2014)... , Dec. 16, 2014 Valencell, a ... its PerformTek biometric technology to industry leaders such as ... accurate, clinically validated, biometric wearable products. These products will ... in Las Vegas . ... is accurate, flexible and robust – with the clinical ...
(Date:12/17/2014)...  Automation is fundamentally transforming the travel experience ... international borders. Over the past decade, ePassports, biometric ... travelers to self process through border control via ... an increasing number of airports, seaports, and land ... to Maxine Most , Principal at Acuity ...
(Date:12/11/2014)... Research and Markets , ... http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition of the ...  report to their offering. One ... in technology. With continuous advances in technology, it ... latest standard that meets the needs and expectations ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... Munich, Germany March 2, 2009 >> Genomatix Software, ... of Next Generation Sequencing (NGS) data, announced today that ... Genomatix Mining Station and a Genomatix Genome Analyzer at ... deliver the only complete and integrated analysis solution for ...
... An international team of scientists, led by Monash University ... kills elderly hospital patients worldwide - a discovery that ... systems billions of dollars each year. The research ... , unravelled ways to genetically modify the bacterium ...
... a picky preschooler who,s avoiding their vegetables? A new Cornell ... like X-Ray Vision Carrots and Tomato Bursts left preschoolers ... carrots called "X-ray Vision Carrots" ate nearly twice as much ... simply labeled as "carrots." The Robert Wood Johnson-funded study also ...
Cached Biology News:Boehringer Ingelheim uses Genomatix' Next Generation Sequencing data analysis systems 2Monash scientists debug superbug 2
sterile filtered, off the clot, all male donors, Total Protein: 4.5 to 8.5 g/dl...
... histochemically detect specific senescence ... in cultured cells and ... marker is only present ... found in presenescent/quiescent/immortal cells. ...
TAR DNA binding protein...
... BL21 Star strains are ... for improved protein yield ... systems (Figure 1). Because ... synthesizes mRNA more rapidly ...
Biology Products: