Navigation Links
Efficiently Insert Unique Restriction Sites into Plasmid Vectors

d Xho I restriction digestion patterns for seven of the clones are shown in Figure 2, indicating that the appropriate restriction sites were successfully introduced into the plasmid vector.

Fig.2

Conclusions

These results demonstrate that a 28-base insertion, encoding three unique restriction enzyme sites, was effectively introduced into a plasmid vector using the QuikChange mutagenesis kit. Mutagenesis reactions and transformation can be completed in less than one day so putative positive clones may be examined the following day. And, because high-fidelity PfuTurbo DNA polymerase is used, random mutations are minimized, rendering this the preferred method for efficient mutagenesis.

REFERENCES
  1. Papworth, C., Bauer, J.C., and Braman, J. (1996) Strategies 9: 3-4.

  2. Hogrefe, H., et al. (1997) Strategies 10: 93-96.

  3. Braman, J. and Basehore, S. (1997) Strategies 10: 84-86.

* U.S. Patent No. 5,789,166 and patents pending
** U.S. Patent Nos. 5,545,552 and 5,866,395 and patents pending


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
2. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
3. A Unique Dimension in Biological Purity
4. Unique Histochemical Substrate Permits Dual Staining In Animal Tissue
5. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
6. Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance
7. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
8. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
9. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
10. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
11. Generate High-Quality, Directional Plasmid cDNA Libraries
Post Your Comments:
(Date:8/26/2015)... WARREN, N.J. , Aug. 26, 2015  Roka ... company focused on providing advanced testing solutions for the ... Thomas, CEO will present at the Sidoti & Company ... The conference will be held at the New York ... the presentation will be available through the investor relations ...
(Date:8/26/2015)... Aug. 26, 2015  Platform therapeutic company Symic ... received a $1.5M Phase II SBIR grant from ... develop its therapeutic agent to reduce arteriovenous fistula ... end stage renal disease (ESRD) patients undergoing hemodialysis. ... of Diabetes and Digestive and Kidney Diseases (NIDDK), ...
(Date:8/26/2015)... -- China Cord Blood Corporation (NYSE: CO ) ... of cord blood collection, laboratory testing, hematopoietic stem cell ... the Company has been informed that Magnum Opus 2 ... Mr. Yuen Kam, chairman of the board of directors ... "Shares") of  Excellent China Healthcare Investment Limited ("KKR SPV") ...
(Date:8/26/2015)... ... ... PRC Clinical, a Clinical Trial Management Expert CRO, will be ... California. The Clinical Trial Management Expert CRO seeks to introduce more California-based sponsors to ... , Stem Cell Meeting on the Mesa on October 7-9 in ...
Breaking Biology Technology:Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 2Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 3China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 2China Cord Blood Corporation Receives Notice of Transactions Regarding its 7% Senior Convertible Notes Due 2017 3PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 2PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 3
... , Multiporator / Electroporator 2510 , , , , , , ... , Protocol No. 4308 915.512 04/2002 , , ... , , Microorganism , ... Cell type , Bacteria, gram negative, , ...
... , , , , , Multiporator , , , , ... Transfection Protocol , Protocol No. 4308 915.022 11/1999 , ... , , , Cell line ... Transfection with , Plasmid RSV/lacZ (in bidistilled H 2 O), ...
... , , , , , , Multiporator / Electroporator 2510 , , ... , Transformation Protocol , Protocol No. 4308 915.509 ... , , , , , , ... , Cell type , ...
Cached Biology Technology:Escherichia coli DH5 2Escherichia coli DH5 3COS-7 2Clostridium botulinum 2
(Date:8/17/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... mobile commerce market and creator of the Wocket® smart ... The new marketing campaign is being spearheaded by an ... ( http://amplitudemarketing.com/ ) and TPMG ( http://tmpg.com/ ). ... driven marketing and brand awareness and has a client ...
(Date:8/11/2015)... -- Today, ZUK announced its Android smartphone Z1, featuring ... revenues in 2015 that relate to sales of FPC1155 for this ... 2,200 MSEK for 2015. Jörgen Lantto, CEO of FPC, ... smartphone manufacturer in China and we ... 155 for Z1 , ...
(Date:8/6/2015)... Calif. , Aug. 6, 2015 ... developer of human interface solutions, today announced collaboration ... technologies on Windows 10. Microsoft leveraged Synaptics, deep ... the operating system. Through stringent ... are fully certified with Microsoft,s Precision TouchPad (PTP) ...
Breaking Biology News(10 mins):NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 2NXT-ID, Inc. Launches Sales Campaign for Wocket Smart Wallet with Award Winning Marketing Team 3FPC's Touch Fingerprint Sensor FPC1155 in ZUK's Smartphone Z1 2Synaptics Chosen for Human Interface Co-Development on Windows 10 2
... Alternating hemiplegia of childhood (AHC) is a very rare ... the body and then the other in devastating bouts ... difficulty walking are common among patients with this diagnosis. ... discovered that mutations in one gene cause the disease ...
... Branch at Galveston researchers have been awarded a two-year, $1.25 ... of custom-growing human lung tissue to make a three-dimensional model ... the size of a dime, will form the core of ... testing new vaccines and drugs and studying disease processes in ...
... This press release is available in German . ... perceive our world in a self-reflective manner is difficult to ... sleep, however, we are not. But there are people, known ... sleep. Studies employing magnetic resonance tomography (MRT) have now been ...
Cached Biology News:Gene discovery set to help with mysterious paralysis of childhood 2Gene discovery set to help with mysterious paralysis of childhood 3UTMB scientists awarded NIH grant for lab-grown lung tissue project 2The seat of meta-consciousness in the brain 2
Mouse monoclonal antibody raised against a partial recombinant ENTPD5. NCBI Entrez Gene ID = ENTPD5...
Mouse monoclonal antibody raised against a partial recombinant TTC3. NCBI Entrez Gene ID = TTC3...
... Protein Staining Solution stains proteins ... electrophoresis (detection limit >= 8 ng ... including washing and destaining processes can ... staining of gel (20 min), proteins ...
Mouse polyclonal antibody raised against a partial recombinant SYT4. NCBI Entrez Gene ID = 6860...
Biology Products: