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phages lambda DNA)
Lane 6: MBI Fermentas GeneRuler (100 bp10 kb)
Conclusion
Using the prepackaged MasterMix with the PCR is characterized by safe and rapid handling: simply add primer and template, and the PCR is ready to go. Reducing pipetting steps not only optimizes the time required but also reduces the risk of contamination and minimizes pipetting errors. Since MasterMix can be stored at 4C, the time-consuming process of thawing reagents is no longer necessary. Special stabilizers make MasterMix extremely thermostable, meaning that temperatures of 20C, room temperature or even 37C have no effect on its performance (see Experiment 2). In comparison to conventional PCR reagents, Eppendorf MasterMix is thus considerably easier to handle in the lab. MasterMix guarantees excellent reproducibility of PCR results (see Experiment 1), with pipetting errors or incorrect preparation of the reagent virtually ruled out.
The consistently high quality of each batch of reaction components, as well as their concentration is subjected to thorough tests during manufacture. MasterMix was successfully tested with a variety of different templates (see Experiment 3), with a wide spectrum of fragments being amplified (160 bp10 kb). Specific, high-yield amplification of very long fragments (10 kb) is also possible using MasterMix without the need to purchase special additional long-range enzymes. Safe to use and with optimized time requirements, MasterMix is ideal for high-throughput laboratories. In addition, its vast applications spectrum and high stability make it the ideal tool for research laboratories that work with different templates and amplicon lengths on a daily basis.
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