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Efficient and Reliable PCR Setup Using Eppendorf MasterMix

8 hours at a deep-freeze temperature, refrigerator temperature, room temperature or thermostat temperature (37C) had no adverse effect on the subsequent performance of the reaction components in the PCR. MasterMix is characterized by maximum thermostability and, at the same time, high processivity and specificity.



Fig. 2: Stability test for Eppendorf MasterMix: Using MasterMix after storage at four different temperatures in three different PCR systems

Lanes 14: A 360-bp fragment of the human apolipoprotein B was amplified from genomic DNA.
Lanes 58: A 547-bp fragment of the pyrophosphatase from Thermus thermophilus HB8 was amplified from genomic DNA.
Lanes 912: A 10-kb fragment from lambda DNA was amplified.
Lane 13: MBI Fermentas GeneRuler (100 bp10 kb)

Experiment 3:
MasterMix shows high performance levels across the entire applications range tested (Fig. 3). Fragments of different sizes can be amplified with high yields and high specificity, irrespective of the template used.



Fig. 3: Applications spectrum of Eppendorf MasterMix with regard to target fragment length and template

Lane 1: 160-bp fragment from exon 26 of the human gene of the apolipoprotein B, (genomic DNA)
Lane 2: 360-bp fragment from exon 26 of the human gene of the apolipoprotein B, (genomic DNA)
Lane 3: 400-bp fragment from the gene of Taq DNA polymerase (60% GC), (plasmid DNA)
Lane 4: 547-bp fragment from the coding sequence of the pyrophosphatase gene Thermus thermophilus (70% GC), (genomic DNA)
Lane 5: 10-kb fragment from the genome of
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