Navigation Links
Efficient and Reliable Linear,,, Amplification of cRNA

lizing T7 RNA polymerase for the preparation of the labeled target, first described by van Gelder and Eberwine [1], has become a standard procedure (Figure 1 a). This protocol consists of a first- and second-strand cDNA synthesis followed by cRNA in vitro transcription using T7 RNA polymerase. For first-strand cDNA synthesis an oligo(dT) primer linked to the T7 promoter sequence is used. In the next step, this T7 linker allows the linear amplification of the template and the simultaneous incorporation of labeled nucleotides with T7 RNA polymerase, generating singlestranded, labeled cRNA. The complete target-preparation workflow consists of multiple enzymatic steps determining the quality and integrity of the targets.

Experimental Validation

To validate the T7 RNA polymerase-based target synthesis procedure, the Roche Applied Science (RAS) Microarray RNA Target Synthesis Kit (T7) and the kit of a major Supplier (A) were tested on three different human acute myeloid leukemia (AML) research samples and one chronic lymphocytic leukemia (CLL) research sample (Figure 1b).

First, 5 g of total RNA was converted into double-stranded (ds) cDNA using the RAS Microarray cDNA Synthesis Kit (eight independent reactions). One group of ds cDNA samples was purified with the RAS Microarray Target Purification Kit, then amplified with T7 RNA polymerase and labeled with Biotin-UTP, using the RAS Microarray RNA Target Synthesis Kit (T7). The resulting labeled cRNA was purified with the RAS Microarray Target Purification Kit. The ds cDNA of the other sample group was purified according to the Supplier As protocol, followed by in vitro transcription and labeling with Biotin- UTP and Biotin-CTP using Supplier As kit. Purification of the labeled cRNA was carried out accor
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
9. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
10. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Efficient and Reliable Linear Amplification cRNA

(Date:5/28/2015)... DUBLIN , May 28, 2015 ... Markets ( http://www.researchandmarkets.com/research/8h5hs2/animal ) has announced the ... "Animal Biotechnology - Technologies, Markets and ... http://photos.prnewswire.com/prnh/20130307/600769) , ,This report describes and ... in veterinary medicine and pharmaceuticals as ...
(Date:5/28/2015)... , May 27, 2015 ... the addition of Jain PharmaBiotech,s new report ... to their offering. An increasing ... biotechnology industries is anticipated. Nanotechnology will be ... - from formulations for optimal delivery to ...
(Date:5/28/2015)... Research and Markets( http://www.researchandmarkets.com/research/l9dqwt/animal_genetics ) has announced ... Products, by Testing services - Global Forecast to 2020" ... animal genetics market is estimated to be worth around ... of 8-9% during the forecast period of 2014 to ... increasing animal protein consumption, increasing adoption of advanced genetic ...
(Date:5/28/2015)... , May 27, 2015 Research ... of Jain PharmaBiotech,s new report "Cell Therapy ... offering. This report describes and evaluates ... started to play an important role in the ... replacing the old fashioned bone marrow transplants. Role ...
Breaking Biology Technology:Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 3Global Nanobiotechnology Market 2015-2024 - Applications, Markets and Companies 2Global Nanobiotechnology Market 2015-2024 - Applications, Markets and Companies 3Global Animal Genetics (Canine, Equine, Poultry, Porcine, Bovine, and Genetic Material) Market 2015 - Global Forecast to 2020 2Global Cell Therapy Market 2015-2020 - Technologies, Markets and Companies 2
... an early-stage medical imaging company developing what it believes to ... had to bypass angel tax credits from the State ... technology. , ,The company instead has raised a total of ... and grants. , ,According to CEO Larry Wells, state law ...
... of a critical state audit, a prominent venture investment executive ... state's Certified Capital Companies. , ,John Neis, co-founder and senior ... findings of the Legislative Audit Bureau are incomplete ... by the so-called CAPCOs, which include Venture Investors, Banc ...
... - Two leaders of The Center for Bioscience ... an entity of Kenosha's Gateway Technical College , have ... open meetings law in a complaint filed by the ... and chairperson Mark Naidicz of BioCATT could pay a maximum ...
Cached Biology Technology:NovaScan decides against tax credits 2Venture capitalist defends Wisconsin CAPCOs 2Venture capitalist defends Wisconsin CAPCOs 3AG accuses BioCATT of violating meetings law 2
(Date:5/28/2015)... , May 28, 2015 Industry ... of a new report titled "Markets for Self-Healing ... polymers, inorganic capsule and vascular systems, biomaterials, relevant ... grow to around $2.7 billion by 2020. ... n-tech,s extensive coverage of smart materials. Other recent n-tech ...
(Date:5/27/2015)... , May 27, 2015   ... medication safety systems and image documentation solutions ... of comprehensive, technologically-advanced automation solutions, is pleased ... integration. The Codonics® Safe Label System® (SLS), ... in conjunction with the Omnicell Anesthesia Workstation, ...
(Date:5/26/2015)... May 26, 2015 Research ... addition of the "Saudi Arabia Biometric Systems ... their offering. ,     (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , ,The biometric ... is projected to grow at over 22% ... is attributed to the surging demand for better ...
Breaking Biology News(10 mins):The Market for Self-Healing Materials to Reach $2.7 Billion by 2020 Says New n-tech Research Study 2The Market for Self-Healing Materials to Reach $2.7 Billion by 2020 Says New n-tech Research Study 3Codonics and Omnicell Expand Partnership 2Codonics and Omnicell Expand Partnership 3Codonics and Omnicell Expand Partnership 4Saudi Arabia Biometric Systems Market Forecast and Opportunities, 2020 with Morpho Safran, ZK Technlogy, and Suprema Dominating 2
... beetles on a quiet day is simple for bats hunting ... must be like trying to locate rustling treats just metres ... impossible to pick out a centipede,s footsteps as a juggernaut ... prey by sound alone cope in our increasingly noisy world ...
... coincided with the emergence and re-emergence of infectious diseases ... the National Science Foundation (NSF) and the National Institutes ... for eight projects under the Ecology of Infectious Diseases ... ninth year of funding. "In a time of ...
... her newborn may be stronger in women who deliver naturally ... a study published by Yale School of Medicine researchers in ... Psychiatry . The researchers, led by Yale Child ... of parents from postpartum wards. One group of 12 mothers ...
Cached Biology News:Bats pick up rustling sounds against highway background noise 2NSF, NIH award Ecology of Infectious Disease grants 2Natural childbirth linked to stronger baby bonding than C-sections 2
Rabbit polyclonal to Presenilin 1, prediluted ( Abpromise for all tested applications). entrezGeneID: 5663 SwissProtID: P49768...
Mouse monoclonal [LPL4A.1] to Plastin L ( Abpromise for all tested applications). SwissProtID: P13796...
... MaV203 Competent Yeast Cells are designed ... (Figure 1). S. cerevisiae strain MaV203 contains ... genes for use with GAL4-based two-hybrid systems. ... mutations for selection of ProQuest bait and ...
Request Info...
Biology Products: