( ...)Efficient and Reliable Linear,,, Amplification of cRNA

Gene Expression Profiling Using Microarray Kits from Roche Applied Science

The use of gene-expression profiling, a genomic application measuring mRNA transcript levels of many genes in parallel, has been growing rapidly over the last few years. Besides the actual microarrays and instrumentation for hybridization and imaging, target preparation is of major importance for reliability and sensitivity of expression-profiling experiments. Several different target preparation strategies have been recently developed that are mainly related to the amount of starting material available.

Benefits of the Roche Applied Science Microarray Kits

• High yield and integrity of labeled cRNA

• Representational amplification of small amounts of RNA for gene-expression analysis

• Seamless workflow from cDNA synthesis to cRNA labeling

• Fast and easy procedure

• Incorporation of hapten- or fluorescence-labeled nucleotides

• Only one labeled nucleotide needed

• Compatible with spotted or in situ synthesized microarrays

Target Preparation Workflow
Especially in medical research applications, sample material is often limited and therefore target amplification is mandatory. Starting from a few micrograms of total RNA, linear amplification of cRNA uti
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