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Efficient Transfection of Neurospora Crassa

Wild Type
(74-OR23-1A) pCSN44 HPH 370 Wild Type
(74-OR23-1A) Negative Control N/A 0 DISCUSSION:

Neurospora crassa has been a model organism in genetic studies for many years. It is an organism that shows great promise for its use in many areas of research including pharmaceuticals, food, molecular biology, genetics, and biochemistry. The emergence of an easier method of transferring exogenous DNA into this organism can only facilitate its use in these areas of study.

Electroporation with the Eppendorf 2510 Electroporator offers a rapid, easy method for transfecting N. crassa conidia. It is no longer necessary to create protoplasts, which are fragile after transfection and may not survive standard electroporation procedures. The method also eliminates the need to treat the conidia with enzymes that weaken the cell wall, which may be required in other electroporation protocols. This procedure is a practical alternative that eliminates the use of toxic chemicals and unnecessary steps.

The transfection results recorded show that this procedure is quick, easy, and efficient. It is also important to note that the steps listed in this protocol make it easily adaptable for use with various selection markers, plasmids, and other types of fungi, with small modifications and adjustments.

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