The diverse reproductive capabilities and the nutritional conventions of Neurospora crassa have made this filamentous fungus a model organism in the field of molecular biology and genetics for more than a half a century. In fact, N. crassa was used in the Nobel Prize-winning "One-gene One-enzyme" theory . Since then, N. crassa has been used as a model organism in other types of molecular and genetic applications, joining the illustrious company of E. coli and Drosphila melanogaster.
Perhaps one of the most important roles forN. crassa has been as a host organism for exogenous DNA. Over the years, several techniques have been developed to introduce exogenous material into cells. Electroporation, a technique utilizing electronically regulated pulses to open the cellular membrane and introduce material, has become an easy and efficient method of transfection.
The Eppendorf® Electroporator 2510 employs electric pulse
technology to provide an easy and efficient means of introducing exogenous
DNA into N. crassa conidia . Most procedures in the past
have either used protoplasts for the transfection of N. crassa, or have
required the addition of an enzyme to facilitate the weakening of the
cell wall. When using the Electroporator 2510, germinated conidia can