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Efficient Recovery of Ultrapure Plasmid DNA

analysis. 100% indicates the total amount of living cells after transfection has taken place. Vector pEGFP; length: 4.7 kb; bacteria strain: JM-109. Each experiment was carried out twice. A: Transfection of COS 7 cells with lipofectamine reagent (Life Technologies). B: Transfection of COS 1 cells with a calcium phosphate transfection kit (Eppendorf 5-Prime). References
  1. Marko MA, Chipperfield R, Birnboim HC. Anal Biochem. 1982;121:382-387.
  2. Manchester KL. Biotechniques. 1995;19:208-210.
  3. Wilfinger WW, Mackey K, Chomczynski P. Biotechniques. 1997;22:474-481.


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