Navigation Links
Efficient Recovery of Ultrapure Plasmid DNA

Mini Kit. Each digestion was carried out using 1 g DNA. 100 l was separated using 1% TAE agarose gel and stained with ethidium bromide. Gibco 1 kb ladder as standard; length: 9.6 kb. At the same time, RNA is degraded effectively by RNase A, which is included in the kits. Also, an enhanced DNA binding matrix1 was specially developed for these kits. In the presence of high concentrations of chaotropic salts, plasmid DNA selectively binds to the surface of the spin-column matrix, whereas polysaccharides and proteins show little or no affinity to the matrix at these salt concentrations. After only two washing steps, the pure plasmid DNA can thus be eluted from the silica matrix under low-salt conditions.2 With the Miniprep kit, the need for additional DNA purification via alcohol precipitation is eliminated, and the DNA is ready to be used for restriction digests, PCR,* or automated sequencing.

Advantages

In tandem with the combination of DNA binding matrix and chaotropic salts, the optimized lysis step maximizes the reproducibility of the results (Fig. 1). The DNA yields are as high as, and in fact usually markedly better than, those obtained with other kits on the market (Fig. 2). More than 95% of the extracted plasmid DNA is in the supercoiled form. Since the purity of the plasmid DNA is similar to DNA purified on a CsCI density gradient, it can be used directly for downstream applications, such as restriction digestion, cloning, transformation, DNA sequencing, or PCR. Even for the transfection of different cell lines (including CHO, COS1, COS7, NIH 3T3, HeLa, and HEK 293), the results obtained are excellent, and they are at least as good a
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
7. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
9. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
10. Efficient and Reliable Linear Amplification of cRNA
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Efficient Recovery Ultrapure Plasmid DNA

(Date:8/21/2014)... for Molecular Medicine Finland (FIMM), University of Helsinki, ... "man and machine" decision support system for diagnosing ... in PLOS One scientific journal today, ... vision algorithms similar to those used in facial ... diagnostically most relevant areas. Tablet computers can be ...
(Date:8/21/2014)... N.Y. , Aug. 21, 2014 ... launch of their Nutraceutical "TELO-20 for Dogs" with ... only Telomere-lengthening supplement in the world for dogs. ... of every chromosome in the body. A wealth ... leading experts in Telomere Science and Aging, including ...
(Date:8/21/2014)... Aug. 21, 2014  China Cord Blood Corporation (NYSE: ... to release financial results for the first quarter of ... market close in the US.  The ... ET on Friday, August 29, 2014 to discuss its ... Company,s recent developments, followed by a question and answer ...
(Date:8/21/2014)... 21, 2014 SoundConnect , ... recognized as one of the nation’s Fastest Growing ... for the 2nd consecutive year. Inc. magazine today ... Inc. 500|5000, an exclusive ranking of the nation's ... comprehensive look at the most important segment of ...
Breaking Biology Technology:A novel 'man and machine' decision support system makes malaria diagnostics more effective 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 2Telomere Biosciences Announces: TELO-20 for Dogs, the World's First Telomere-Lengthening Supplement for Dogs, to Promote Healthy Aging and Longevity 3China Cord Blood Corporation to Report First Quarter of Fiscal 2015 Financial Results 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3
... INDIANAPOLIS, Sept. 23 The volume of biological samples ... pharmaceutical and biotechnology industries shift their focus from traditional ... and biomarkers in the discovery process has put an ... the practices that life sciences companies employ to collect, ...
... Pharmaceutical and Biotech Trainers (SPBT) today announced that Kevin ... October 1.  He brings to his new role significant ... Mr. Kruse will be responsible for leading ... organization that represents more than 1,000 members in the ...
... 23, 2010 ... ... var shortURL = ... so we need to grab the first one. for (var r in data.results) { first_result = data.results[r]; ...
Cached Biology Technology:BioStorage Technologies Brings Together Industry Leaders for Global Sample Management Benchmarking Symposium 2BioStorage Technologies Brings Together Industry Leaders for Global Sample Management Benchmarking Symposium 3Kevin Kruse Named Executive Director of the Society of Pharmaceutical and Biotech Trainers 2BioSpain 2010 Will Host 2,000 Business Meetings in Which More Than 600 Companies Will Come Together 2BioSpain 2010 Will Host 2,000 Business Meetings in Which More Than 600 Companies Will Come Together 3BioSpain 2010 Will Host 2,000 Business Meetings in Which More Than 600 Companies Will Come Together 4BioSpain 2010 Will Host 2,000 Business Meetings in Which More Than 600 Companies Will Come Together 5BioSpain 2010 Will Host 2,000 Business Meetings in Which More Than 600 Companies Will Come Together 6BioSpain 2010 Will Host 2,000 Business Meetings in Which More Than 600 Companies Will Come Together 7
(Date:8/21/2014)... including researchers from the University of Georgia recently published the ... journal Science . Their discovery paves the way for ... farming and industry. , Canola is grown across much ... is increasingly cultivated in Georgia. Canola oil used for cooking ... and rich supply of omega-3 fatty acids, but the plant ...
(Date:8/21/2014)... use a previously unknown process for harvesting energy and ... a Penn State University scientist has discovered. The discovery ... plant growth, harvesting energy from the Sun, and understanding ... and other lakes worldwide. A paper describing the discovery ... edition of the journal Science on 21 ...
(Date:8/21/2014)... food intake, health and quality of life of older ... at the University of Waterloo receiving close to $1.5 ... Research (CIHR). , Professor Heather Keller, of the ... Schlegel research chair in nutrition and aging, will receive ... living in long-term care homes are poorly nourished. The ...
Breaking Biology News(10 mins):Canola genome sequence reveals evolutionary 'love triangle' 2Hot-spring bacteria reveal ability to use far-red light for photosynthesis 2Hot-spring bacteria reveal ability to use far-red light for photosynthesis 3Grants will fund landmark aging research at Waterloo 2
... to dash after food flakes at mealtime, reaching them before ... MIT recently proved that marine bacteria, the smallest creatures in ... their swimming skills to reach tiny food patches that appear ... bacteria at these small scales could have global implications, possibly ...
... with bacteria from the Chesapeake Bay has led to a ... all kinds of plant products, from leftover brewers mash to ... , That ... Ron Weiner, is the foundation of their incubator company Zymetis, ...
... Abdullah University of Science and Technology (KAUST) and ... Academic Excellence Alliance (AEA) , designed to establish ... the academic curriculum, and identify and nominate the founding ... for the new University. KAUST is ...
Cached Biology News:Marine bacteria's mealtime dash is a swimming success 2Marine bacteria's mealtime dash is a swimming success 3Trash today, ethanol tomorrow 2KAUST announces academic excellence alliance partnership with Imperial College London 2
WASF2 Immunogen: WASF2 (NP_008921, 73 a.a. ~ 173 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
... to ThPok ( Abpromise for all tested ... conjugated to KLH derived from within residues 500 ... the amino acid sequence is proprietary) ... Entrez Gene ID: 51043 ...
Applications: Western blotting Stability: Room temperature, indefinitely ...
Rabbit polyclonal to SUR1 ( Abpromise for all tested applications). Antigen: Synthetic peptide Entrez Gene ID: 6833 Swiss Protein ID: Q09428...
Biology Products: