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Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN ,,, siRNA and TransMessenger Transfection Reagent*

clusions
  • Our results show that TransMessenger Transfection Reagent can be used to successfully transfect primary neuronal cells with synthetic QIAGEN siRNA and effectively suppress target gene expression. Transfection of synthetic siRNA using the non-liposomal TransMessenger Reagent is an attractive alternative to transfection using other lipid-based reagents, which are often toxic to neuronal cells.

  • As primary neuronal cells are difficult to transfect with DNA, the high transfection efficiency acheived using siRNA and TransMessenger Transfection Reagent (7080%) suggests that neuronal cells, at least, are better suited to RNAi experiments using synthetic siRNA than RNAi experiments that require plasmid DNA to be transfected.

  • The application of RNAi techniques to neurons, a setting where genetic manipulations have traditionally proven difficult, will be a valuable tool in studies on neuronal gene-regulation and function.
References
  1. Krichevksy, A., and Kosik, K. (2002) RNAi functions in cultured mammalian neurons.

  2. Proc. Natl. Acad. Sci. USA 99, 11926. Gonzalez-Billault, C. (2002) Participation of structural microtubuleassociated proteins (MAPs) in the development of neuronal polarity. J. Neurosci. Res. 67, 713.

  3. Caceres, A., Muatinoem J., and Kosik, K. (1992) Suppression of MAP2 in cultured cerebellar macroneurons inhibits minor neurite formation. Neuron. 9, 607.


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