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Efficient Delivery of siRNAs to Human ,,, Primary Cells: Electroporation ,,, vs. Chemical Transfection

ression was calculated as a percentage of gene expression compared with the negative control siRNA. Duplicates were performed for each sample.


Our results demonstrate that electroporation provides an efficient non-viral method for delivering siRNAs in at least these three types of primary cells. In contrast to chemical transfection reagents, electroporation of a GAPDH siRNA reduced GAPDH expression by greater than 90% across several primary cell types, including NHEK cells, which are only poorly transfected with several common chemical transfection agents. The application of siRNA to primary cell types, a setting where genetic manipulations have traditionally proven difficult, will be a valuable tool in studies on target validation, gene discovery, and gene therapeutic approaches.


Note that it is common to see relative variations in data when conducting tissue culture experiments. Reproducibility can be achieved by rigorously following protocols.

Cy is a trademark of Amersham Biosciences.

siPORT Amine is manufactured for Ambion by Mirus.

TaqMan is a registered trademark of Applied Biosystems.


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