Navigation Links
Efficient Delivery of siRNAs to Human ,,, Primary Cells: Electroporation ,,, vs. Chemical Transfection

Dmitriy Ovcharenko (Research Associate II, Ambion, Inc.)

Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells are often desired for such experiments because they are more similar to their in vivo counterparts than are immortalized cells. The use of siRNAs in human primary cells continues to accelerate applications such as target validation, gene discovery, and gene therapeutic approaches that could lead to powerful new gene-specific siRNA-based therapeutics.

Chemical transfection is the standard method for introducing siRNA into immortalized cells. Unfortunately, efficient transfer of siRNAs into primary cells by chemical transfection has so far been restricted to a few cell types only. This is illustrated in Figure 1, below. Primary cells tend to be more difficult to transfect chemically in general, which has limited their use for siRNA experiments. Here we address the use of electroporation as an alternative for efficient delivery of siRNAs into primary cell types.


Chemical Transfection: Inefficient siRNA Delivery in Primary Cells
To show that chemical transfection is only useful as an siRNA delivery method for only certain types of primary cells, an siRNA targeting GAPDH was transfected into normal human umbilical vein endothelial cells (HUVEC) and normal human epidermal
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
9. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
10. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
11. Efficient and Reliable Linear Amplification of cRNA
Post Your Comments:
(Date:10/22/2014)... NJ (PRWEB) October 22, 2014 ... and services – has been working with a team ... and New Haven Farms Community Farming Collaborative to provide ... ePRO solution. The study, which started in May, ... program that provides participants weekly exposure to and participation ...
(Date:10/22/2014)... (PRWEB) October 22, 2014 Grace Century, ... and healthcare projects, announces the addition of Dr. ... to its advisory team. Dr. Siddiqui will provide further ... , A graduate of University College Medical School ... degreed in medicine in 2001. With further certification as ...
(Date:10/22/2014)... 22, 2014 According to new ... Service Type (Consulting, Integration, Deployment & Support), by ... Model (Public, Private, Others (Hybrid & Community)) - ... and segments the Cloud Professional Services Market into ... of revenues. It also identifies the drivers and ...
(Date:10/22/2014)... /PRNewswire/ - iCo Therapeutics ("iCo" or "the Company") (TSX-V: ... its Oral Amphotericin B program.  The company recently ... work involving samples from HIV/AIDS patients exposed ... pre clinical studies and regulatory filings to move ... utilizing approximately $700,000 of funding and technological advice ...
Breaking Biology Technology:myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3Dr. Yousef Siddiqui joins the Grace Century Advisory Team 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3
... Aoxing Pharmaceutical,Company, Inc. (OTC Bulletin Board: CAXG), which ... highly regulated narcotic,medicines and pain medication in China, ... fiscal 2008 ended September 30, 2007. China ... first quarter of fiscal 2008, a 33% sequential ...
... Convergence and New Opportunities, NEW BRUNSWICK, N.J., Nov. ... organizational changes to sharpen its,focus on opportunities outside its ... health care. These include the creation of a new,strategy ... The,changes are designed to accelerate growth for Johnson & ...
... 14 Advanced Life Sciences,Holdings, Inc. (Nasdaq: ... development and commercialization of novel drugs in the,therapeutic ... its financial results for the third quarter ended ... The net loss for the three months ended ...
Cached Biology Technology:China Aoxing Pharmaceutical Reports Financial Results for the First Quarter of Fiscal 2008 2China Aoxing Pharmaceutical Reports Financial Results for the First Quarter of Fiscal 2008 3China Aoxing Pharmaceutical Reports Financial Results for the First Quarter of Fiscal 2008 4China Aoxing Pharmaceutical Reports Financial Results for the First Quarter of Fiscal 2008 5Johnson & Johnson Announces Changes to Accelerate Growth 2Johnson & Johnson Announces Changes to Accelerate Growth 3Advanced Life Sciences Announces Third Quarter 2007 Financial Results 2Advanced Life Sciences Announces Third Quarter 2007 Financial Results 3Advanced Life Sciences Announces Third Quarter 2007 Financial Results 4Advanced Life Sciences Announces Third Quarter 2007 Financial Results 5Advanced Life Sciences Announces Third Quarter 2007 Financial Results 6Advanced Life Sciences Announces Third Quarter 2007 Financial Results 7Advanced Life Sciences Announces Third Quarter 2007 Financial Results 8Advanced Life Sciences Announces Third Quarter 2007 Financial Results 9
(Date:10/22/2014)... 2014  Leading identity analyst firm Acuity Market Intelligence ... world,s population will have a chip-based National eID card, ... . Asia , with its vast ... more than 60% of all National eID cards issued ... "The Global National eID Industry Report: 2014 ...
(Date:10/19/2014)... in India over the last 30 years contributed ... growth during that time, according to a new ... Change ., "Energy access is fundamental to development: ... including education, communication, and health," says IIASA researcher ... increased energy access is widely agreed to be ...
(Date:10/18/2014)... referred for evaluation of suspected genetic conditions, whole-exome ... percent, including detection of a number of rare ... according to a study appearing in JAMA ... with the American Society of Human Genetics annual ... coding regions of thousands of genes simultaneously using ...
Breaking Biology News(10 mins):Half the World's Population Will Have Chip-Based National Electronic Identity (eID) Cards by 2018 2Improved electricity access has little impact on climate change 2Whole-exome sequencing shows potential as diagnostic tool 2
... of a protein that enables malaria parasites to invade ... rational antimalarial drug design and vaccine development. The findings ... journal Cell. , Malaria causes approximately 400 million clinical ... 80% of deaths occurring among children. The disease is ...
... to zoom in on smaller sections of cell chromosomes ... identified nearly 100 chromosome regions where genes are either ... findings provide new clues about the location of genes ... cancer –?and one of the deadliest of all malignancies ...
... that instructs cells with fatally damaged DNA to self-destruct pivots ... as it is in over half of all human cancers, ... body cells start to accumulate mutations, which ultimately may lead ... has been studied in great detail for many years but ...
Cached Biology News:Malaria mechanism revealed 2High-powered gene profiles provide clues to genes involved in common form of lung cancer 2High-powered gene profiles provide clues to genes involved in common form of lung cancer 3Cancer related gene p53 not regulated as indicated by previous tissue culture research 2Cancer related gene p53 not regulated as indicated by previous tissue culture research 3
... U.S. sourced, ideal for those ... Low hemoglobin; Endotoxin: < 1.0 ... US origin; U.S. sourced, ideal ... uncompromising quality. Low hemoglobin Endotoxin: ...
Chicken polyclonal to ytzF ( Abpromise for all tested applications). Antigen: Full length protein...
Fetal Calf Serum Replacement (Bistec) serum...
...
Biology Products: