Navigation Links
Efficient Delivery of siRNAs to Human ,,, Primary Cells: Electroporation ,,, vs. Chemical Transfection

Dmitriy Ovcharenko (Research Associate II, Ambion, Inc.)

Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells are often desired for such experiments because they are more similar to their in vivo counterparts than are immortalized cells. The use of siRNAs in human primary cells continues to accelerate applications such as target validation, gene discovery, and gene therapeutic approaches that could lead to powerful new gene-specific siRNA-based therapeutics.

Chemical transfection is the standard method for introducing siRNA into immortalized cells. Unfortunately, efficient transfer of siRNAs into primary cells by chemical transfection has so far been restricted to a few cell types only. This is illustrated in Figure 1, below. Primary cells tend to be more difficult to transfect chemically in general, which has limited their use for siRNA experiments. Here we address the use of electroporation as an alternative for efficient delivery of siRNAs into primary cell types.


Chemical Transfection: Inefficient siRNA Delivery in Primary Cells
To show that chemical transfection is only useful as an siRNA delivery method for only certain types of primary cells, an siRNA targeting GAPDH was transfected into normal human umbilical vein endothelial cells (HUVEC) and normal human epidermal
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
9. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
10. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
11. Efficient and Reliable Linear Amplification of cRNA
Post Your Comments:
(Date:7/22/2014)... 22, 2014 The Board of Directors of BD ... declared a quarterly dividend of 54.5 cents per common share ... September 9, 2014. The indicated annual dividend rate is $2.18 ... is a leading medical technology company that partners with customers ... and evolving health needs. Our innovative solutions are focused on ...
(Date:7/22/2014)... within a phylum so full of mean little creatures, the ... its cruelty -- at least to crickets. Native to the ... most predatory sort of parasite. It swoops onto the back ... and leaves its wicked brood to invade, kill and consume ... would be possible without the fly,s ability to find a ...
(Date:7/22/2014)... Institute of Standards and Technology (NIST) and California ... design for an atomic clock that is based ... , The microcomb clock, featured on the cover ... Optica ,* is the first demonstration of ... conversion of optical frequencies to lower microwave frequencies. ...
(Date:7/22/2014)... in water with ultrasound and they,ll spin around their ... yet knows exactly. But researchers at the National Institute ... it,s fast. At up to 150,000 revolutions per minute, ... object submerged in liquid ever reported. , The discovery ... that they could be used not only for moving ...
Breaking Biology Technology:Fly-inspired sound detector 2Fly-inspired sound detector 3Fly-inspired sound detector 4'Comb on a chip' powers new NIST/Caltech atomic clock design 2NIST shows ultrasonically propelled nanorods spin dizzyingly fast 2
... 2 SAFC Hitech(TM), a focus,area within SAFC(R), ... SIAL ),today announced the introduction of the OM700, ... to controlled precursor delivery,for larger lot sizes, enabling ... controls., The OM700 offers reliable and very ...
... geographic scales ranging from vast climatic regions of the Earth ... of soil nano-particles in a pinch of soil. , ... properties vary from the summit of a hill down to ... less than a quarter inch in diameter, there is a ...
... major U.S. cancer conference, CHICAGO, May 30 ... evolving field of molecular diagnostics, announced today that ... prognosis test,will be presented at the American Society ... show that MammaPrint, a,prognostic test for breast cancer ...
Cached Biology Technology:SAFC Hitech Introduces Large Scale Bubbler for Compound Semiconductor Production 2SAFC Hitech Introduces Large Scale Bubbler for Compound Semiconductor Production 3Sophisticated soil analysis for improved land use 2MammaPrint(R) Breast Cancer Test Provides Valuable Insight for Personalized Treatment Decisions 2MammaPrint(R) Breast Cancer Test Provides Valuable Insight for Personalized Treatment Decisions 3MammaPrint(R) Breast Cancer Test Provides Valuable Insight for Personalized Treatment Decisions 4
(Date:7/22/2014)... uncanny precision are key parts of high-powered lenses, ... instead of electricity. But producing these crystals by ... precise beam of electrons, is often extremely difficult ... Columbia universities have proposed a new method that ... specialized materials, known as photonic crystals, with relative ...
(Date:7/22/2014)... kids will hear that drinking milk helps make their bones strong ... messages foster the idea that if something is good for us, ... the Journal of Consumer Research , when children hear about ... , "We predicted that when food is presented to children as ... such as learning how to read or count, they would conclude ...
(Date:7/22/2014)... that feature natural habitats and trees and not just ... according to a University of Colorado Boulder study. , ... also provide stress-reducing benefits for youth, according to a ... The study is one of the first of ... access to green settings and stress. , "Many schools ...
Breaking Biology News(10 mins):Technique simplifies the creation of high-tech crystals 2Technique simplifies the creation of high-tech crystals 3Natural-terrain schoolyards reduce children's stress, says Colorado University-Boulder study 2Natural-terrain schoolyards reduce children's stress, says Colorado University-Boulder study 3
... to newly placed sod may result in nutrient runoff into ... to apply fertilizer and what kind is the best for ... team of scientists from Texas A&M AgriLife Research: Dr. Jacqui ... Ben Wherley, assistant professor of turfgrass science and ecology; Dr. ...
... reverse a principal cause of global warming? A new study ... population that keeps sea urchins in check will in turn allow kelp ... times the amount of CO 2 from the atmosphere than if ... The theory is outlined in a paper released online today (September ...
... North Carolina State University shows that exposure to the chemical ... of anxiety by causing significant gene expression changes in a ... also found that a soy-rich diet can mitigate these effects. ... variety of species, and wanted to begin to understand why ...
Cached Biology News:Turf study to monitor runoff, establish fertilizer management practices 2UC Santa Cruz study shows how sea otters can reduce CO2 in the atmosphere 2Study finds how BPA affects gene expression, anxiety; Soy mitigates effects 2
Denhardt's Solution is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains 1% Ficoll (type 400) 1% polyvinylpyrrolidone and 1% bovine serum albumin. Ste...
... 97 Cytological Labeling Kit provides a unique ... of cellular targets, including cell-surface receptors and ... Kit contains the patented ELF 97 phosphatase ... precipitate at the site of enzymatic activity ...
... H 10 O 2 S 2 ... Form: White crystalline powder Assay: greater ... By IR Melting Point: greater than ... less than or equal to 1% ...
... consists of an anodized aluminum body in ... Compounds embedded in polymerized matrix. It is ... and Synergy Multi-Detection Readers. This multi-functional test ... reducing the need for tedious wet testing. ...
Biology Products: