Navigation Links
Efficient Delivery of siRNAs to Human ,,, Primary Cells: Electroporation ,,, vs. Chemical Transfection

Dmitriy Ovcharenko (Research Associate II, Ambion, Inc.)

Gene silencing using small interfering RNAs (siRNAs) has become a powerful method for studying gene function. Human primary cells are often desired for such experiments because they are more similar to their in vivo counterparts than are immortalized cells. The use of siRNAs in human primary cells continues to accelerate applications such as target validation, gene discovery, and gene therapeutic approaches that could lead to powerful new gene-specific siRNA-based therapeutics.

Chemical transfection is the standard method for introducing siRNA into immortalized cells. Unfortunately, efficient transfer of siRNAs into primary cells by chemical transfection has so far been restricted to a few cell types only. This is illustrated in Figure 1, below. Primary cells tend to be more difficult to transfect chemically in general, which has limited their use for siRNA experiments. Here we address the use of electroporation as an alternative for efficient delivery of siRNAs into primary cell types.


Chemical Transfection: Inefficient siRNA Delivery in Primary Cells
To show that chemical transfection is only useful as an siRNA delivery method for only certain types of primary cells, an siRNA targeting GAPDH was transfected into normal human umbilical vein endothelial cells (HUVEC) and normal human epidermal
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
9. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
10. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
11. Efficient and Reliable Linear Amplification of cRNA
Post Your Comments:
(Date:1/22/2015)... Protocol Networks brings independent technology consulting to the ... consultants, Protocol Networks has recently announced its expansion into ... his company has attracted several clients in The Constitution ... as others. With the success of these engagements, Rogers ...
(Date:1/22/2015)... January 22, 2015 Dr. Greg Leyer of ... annual Scripps Natural Supplements Pre-Conference seminar on probiotics in San ... an annual continuing education conference for health care professionals. This ... the topic of probiotics in health. Dr. Leyer spoke about ...
(Date:1/22/2015)... Nueva Jersey , 22 de enero de 2015  El ... abre hoy su llamada a nominaciones para 2015. Este ... ha hecho, o tiene el potencial para hacer, contribuciones ... nominaciones se aceptarán hasta el 15 de marzo de ...
(Date:1/22/2015)... N.J. , Jan. 22, 2015  Derma Sciences, ... regeneration company focused on advanced wound care, announces ... placental tissues, have been added to the Premier, ... increases the opportunity for the AMNIOEXCEL® and AMNIOMATRIX® ...
Breaking Biology Technology:Protocol Networks Brings Independent Technology Consulting to the Constitution State 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5
... Inc. (CTi), a federal government professional consulting firm, announced ... web-based services in support of DoD,s Blast Injury Research ... International Corporation (NYSE: SAI ) and the ... U.S. Army Medical Research and Materiel Command (USAMRMC), CTi ...
... extensive experience in multi-GPU high-performance computing systems ... provide scientists, engineers and researchers with a ... Supercomputer (PSC), which will be shown at ... SC08 Conference, the top international conference for ...
... ParkwayHealth is the first healthcare group in Southeast Asia to offer ... , ... Singapore (PRWEB) November 18, 2008 -- Singapore,s ParkwayHealth ... In-Space percutaneous interspinous distraction device, a revolutionary spinal procedure ., ...
Cached Biology Technology:Cascades Technologies, Inc. Chosen to Deliver IT Support on a DoD Blast Injury Research Program 2Cascades Technologies, Inc. Chosen to Deliver IT Support on a DoD Blast Injury Research Program 3BOXX Personal Supercomputer for Research Scientists Featured at SC08 2Singapore Medicine - Singapore Doctors Use Hi-Tech Implant to Add Space to Narrowed Spinal Canals 2Singapore Medicine - Singapore Doctors Use Hi-Tech Implant to Add Space to Narrowed Spinal Canals 3Singapore Medicine - Singapore Doctors Use Hi-Tech Implant to Add Space to Narrowed Spinal Canals 4
(Date:12/22/2014)... , Dec. 22, 2014 Research and Markets ... addition of the "The Global Watermarking and ... http://photos.prnewswire.com/prnh/20130307/600769 ... digital media watermarking and fingerprinting markets. Watermarking aims ...
(Date:12/19/2014)... and Markets ( http://www.researchandmarkets.com/research/8kmfcn/iphone_5s ) has announced ... - Apple/AuthenTec TMDR92 & Sapphire - Technology Report" ... http://photos.prnewswire.com/prnh/20130307/600769 Following the acquisition of AuthenTec ... with the iPhone 5S. It is currently the only ...
(Date:12/19/2014)... Dec. 18, 2014  23andMe, Inc., the leading personal genetics company, ... differences in genetic ancestry of individuals from across ... arrived more than four hundred years ago, the ... for peoples from different continents. This study illuminates how American ...
Breaking Biology News(10 mins):The Global Watermarking and Fingerprinting Markets 2iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 223andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4
... Office of Science has established a program to ... Katrina. , "Our colleagues in science have historically ... L. Orbach, director of DOE's Office of Science, ... universities and colleges, including those in Alabama, Louisiana ...
... Analysis of a key protein in different subtypes of ... drug amantadine in H5N1 occurs worldwide, but is especially ... to the antiviral drug amantadine is spreading more rapidly ... Asia than in North America, according to the study ...
... A new website with a Global Information System will ... Hurricane Katrina. The National Institute of Environmental Health Sciences ... the website to provide the most up-to-date data to ... waters, infrastructure and industry maps, as well as demographic ...
Cached Biology News:DOE's Office of Science sets up program to aid scientists displaced by Hurricane Katrina 2Drug resistant avian influenza viruses more common in Southeast Asia than North America 2Drug resistant avian influenza viruses more common in Southeast Asia than North America 3Drug resistant avian influenza viruses more common in Southeast Asia than North America 4FDA approves child-friendly AIDS medicine 2
Mouse polyclonal antibody raised against a partial recombinant DDEF2. NCBI Entrez Gene ID = 8853...
Mouse monoclonal antibody raised against a partial recombinant ALAS2. NCBI Entrez Gene ID = ALAS2...
Mouse polyclonal antibody raised against a partial recombinant QPCT. NCBI Entrez Gene ID = 25797...
... raised against a partial recombinant IFNA2. ... recombinant protein is produced in E. coli. ... IU/ mg. OMIM: 147562, ... Number: MM951100564 MA Code: ...
Biology Products: