Navigation Links
Efficient DNA transfection of primary CNS neurons using TransMessenger ,,, Transfection Reagent

taining; G: transfected cells, GFP fluorescence; H: superimposed images from F and G. Cells were transfected in a 96-well format with 0.3 g DNA and 2 l TransMessenger Reagent per well.

The superimposed images in Figure "Excellent Morphology of CryoCell Cultures" show that the cells expressing GFP are positive for MAP2 and negative for GFAP and are therefore neurons. In this typical visual field, approximately 10% of the neurons are transfected. In general, the efficiency of transfection in cultured CryoCells was 5-10%. This is a high efficiency for neurons, which are notoriously difficult to transfect. The transfected neurons are again well differentiated and GFP fluorescence is seen in their neurite network and somata. "Excellent Morphology at Higher Magnification" illustrates the excellent morphology of transfected neurons.

Excellent Morphology at Higher Magnification Cortical neurons 24 hours after transfection with a plasmid encoding GFP. Cells were stained with anti-MAP2 primary antibodies and Cy3-labeled secondary antibodies. A: Anti-MAP2 staining; B: GFP fluorescence; C: superimposed images. Cells were transfected in a 96-well format using 0.3 g of DNA and 2 l of TransMessenger Reagent per well.

Conclusions
  • Although primarily recommended for transfection of RNA, TransMessenger Reagent can be used to efficiently transfect DNA into primary cortical neurons, enabling genetic modification of these cells.
  • CryoCells from rat brain cortex can be used to generate high-quality cultures of primary cortical rat neurons, and are a convenient and practical su
    '"/>


Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
9. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
10. Efficient and Reliable Linear Amplification of cRNA
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
Post Your Comments:
(Date:1/22/2015)... Protocol Networks brings independent technology consulting to ... independent consultants, Protocol Networks has recently announced its expansion ... years, his company has attracted several clients in The ... well as others. With the success of these engagements, ...
(Date:1/22/2015)... MA (PRWEB) January 22, 2015 zFlo ... is pleased to announce an exciting product from a new ... motion analysis solutions over the past 15 years, with offerings ... Most recently, their 3D IMU system (inertial measurement unit), iSen, ...
(Date:1/22/2015)... 22, 2015 Selexis SA, a serial ... Cell Banks (RCBs) used for drug discovery to commercial ... will include Next-Generation Sequencing (NGS) data packages. ... biologic manufacturing by ensuring the integrity of the gene, ...
(Date:1/22/2015)... 2015   Cypher Genomics, Inc., the leading ... SQNM ), the leading molecular diagnostics company, ... noninvasive prenatal tests (NIPT). Through this agreement, Sequenom ... to advance analysis of clinically-relevant fetal sub-chromosomal variants ...
Breaking Biology Technology:Protocol Networks Brings Independent Technology Consulting to the Constitution State 23D Motion Capture Just Got Easy 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2Cypher Genomics and Sequenom Announce Development Agreement 2Cypher Genomics and Sequenom Announce Development Agreement 3Cypher Genomics and Sequenom Announce Development Agreement 4
... L.J. Star , Inc., a major supplier ... it has a suite of solutions for the special demands of process ... , , ... monitor fluid flows, and thereby help maintain strict control of critical processes. ...
... soon be able to surf in colder waters and ... Laboratory (NPL) and UK wetsuit manufacturer, Spartan. Spartan ... approached NPL, the UK,s measurement institute, to understand the ... its product design. In particular it wanted to improve ...
... AMSTERDAM , May 19, 2010 ... field of human gene,therapy, today provides its non-audited business update in ... financial position for the first quarter of 2010. , , ... formal review of Glybera(R) dossier, - Dossier validated ...
Cached Biology Technology:L.J. Star Announces Stainless Sanitary Fittings, Sight Glasses and Sight Flow Indicators for the Special Demands of the Pharmaceutical Industry 2L.J. Star Announces Stainless Sanitary Fittings, Sight Glasses and Sight Flow Indicators for the Special Demands of the Pharmaceutical Industry 3L.J. Star Announces Stainless Sanitary Fittings, Sight Glasses and Sight Flow Indicators for the Special Demands of the Pharmaceutical Industry 4A warmer future for watersports 2AMT Provides Business Update for the First Quarter 2010 2AMT Provides Business Update for the First Quarter 2010 3
(Date:1/22/2015)... CHICAGO , Jan. 9, 2015 Valid S.A. ("Company") ... the market in general the State of Washington,s ... contract for a new enrollment and central issuance system for ... The project planning and development will start ...
(Date:1/22/2015)... Jan. 16, 2015  A man-made form of insulin delivered ... mental capabilities in adults with mild cognitive impairment and ... by researchers at Wake Forest Baptist Medical Center. ... amnesic mild cognitive impairment (MCI) or mild to moderate ...
(Date:1/22/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/wxdglm/global_wearable ... "Global Wearable Technologies Market and Applications, Opportunities, Industry ... Forecast 2014-2020" report to their offering. ... that can be worn on the user,s body ...
Breaking Biology News(10 mins):Valid USA Signs Contract For Washington Driver's License Issuance 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Global Wearable Technologies Market and Applications, Opportunities, Industry Analysis, Segmentation, Size, Growth, Share, Overview, Trends and Forecast 2014-2020 2
... , This release is available in ... of Agriculture (USDA) scientists and cooperators provides new details ... antibacterial agent in soaps and other cleaning suppliesinto the ... as treated wastewater solids, to their fields as a ...
... X-rays are the medium of choice for many scientific ... shed light on the material,s structure, providing loads of information ... the sample has to be in a vacuum for the ... liquids, that means you have to remove all the water. ...
... Each year, thousands of acres of crops are planted throughout ... by a parasitic plant called Striga, also known as witchweed. ... security in Africa, and a team of scientists led by ... genes that may break Striga,s stranglehold. When crops grow, ...
Cached Biology News:Tracking triclosan's field footprint 2LiXEdrom: Innovative measuring chamber for X-ray study of liquid jets 2
Candida (IgA) -Ab EIA Sample Size: 5 l...
... cell lines in combination with the ... parameters. Nucleofector Kits are only functional ... Different cell lines can be transfected ... and viabilities are given in brackets ...
...
Request Info...
Biology Products: