Navigation Links
Efficient DNA transfection of primary CNS neurons using TransMessenger ,,, Transfection Reagent

taining; G: transfected cells, GFP fluorescence; H: superimposed images from F and G. Cells were transfected in a 96-well format with 0.3 g DNA and 2 l TransMessenger Reagent per well.

The superimposed images in Figure "Excellent Morphology of CryoCell Cultures" show that the cells expressing GFP are positive for MAP2 and negative for GFAP and are therefore neurons. In this typical visual field, approximately 10% of the neurons are transfected. In general, the efficiency of transfection in cultured CryoCells was 5-10%. This is a high efficiency for neurons, which are notoriously difficult to transfect. The transfected neurons are again well differentiated and GFP fluorescence is seen in their neurite network and somata. "Excellent Morphology at Higher Magnification" illustrates the excellent morphology of transfected neurons.

Excellent Morphology at Higher Magnification Cortical neurons 24 hours after transfection with a plasmid encoding GFP. Cells were stained with anti-MAP2 primary antibodies and Cy3-labeled secondary antibodies. A: Anti-MAP2 staining; B: GFP fluorescence; C: superimposed images. Cells were transfected in a 96-well format using 0.3 g of DNA and 2 l of TransMessenger Reagent per well.

Conclusions
  • Although primarily recommended for transfection of RNA, TransMessenger Reagent can be used to efficiently transfect DNA into primary cortical neurons, enabling genetic modification of these cells.
  • CryoCells from rat brain cortex can be used to generate high-quality cultures of primary cortical rat neurons, and are a convenient and practical su
    '"/>


Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
9. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
10. Efficient and Reliable Linear Amplification of cRNA
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
Post Your Comments:
(Date:10/31/2014)... , Oct. 31, 2014  Tavistock Development ... by Tavistock Group, celebrated the groundbreaking of the ... City. The 92,000-square-foot, three-story facility ... Central Florida designed specifically for life ... the heart of the 650-acre Lake Nona Medical ...
(Date:10/30/2014)... 2014  Ardelyx, Inc. (NASDAQ: ARDX ... gastrointestinal and metabolic diseases, today announced that it ... Thursday, November 6, 2014. Following the announcement, the ... call and webcast at 4:30pm ET to review ... update. The live webcast can be ...
(Date:10/30/2014)... SoundConnect , an industry leading ... end users the key to turning this already ... adding voice enablement and hybrid audio conferencing. , ... communication tool that includes presence, instant messaging and ... becomes a comprehensive unified communications solution. Adding the ...
(Date:10/30/2014)... (PRWEB) October 30, 2014 Whitehouse Laboratories ... its ICH Stability Testing and Package Test Capacity. Responding ... centers on a dramatic increase in environmental chamber storage ... stability, storage and aging samples to be securely held ... this major capital expenditure has already taken place with ...
Breaking Biology Technology:GuideWell Innovation Center Groundbreaking Hosted by Tavistock Development Company at Lake Nona Medical City 2GuideWell Innovation Center Groundbreaking Hosted by Tavistock Development Company at Lake Nona Medical City 3Ardelyx to Report Third Quarter 2014 Financial Results on November 6, 2014 2SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 2SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 3Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 2Whitehouse Labs Announces Major Capital Investment in Stability and Storage Testing Capacity 3
... an important role in many cases of Parkinson,s disease (PD). ... Journal of Neuroscience , researchers from the Ecole Polytechnique ... model for studying a specific gene mutation commonly found in ... the malady. , "This is a great step forward toward ...
... shed water so effectively that they don,t need wipers. ... the water more efficiently than ordinary hulls. These are ... the hottest new materials in the field of nanotechnology, ... of physics at Vanderbilt. Dickerson and his colleagues have ...
... SAN MARINO, Calif., Feb. 1, 2011 Boston-based ... (Pink Sheets: VRAL ) and published ... on the company,s website at www.viralgenetics.com/investors/press-releases/R20Report ... through institutional platforms including FactSet, CapitalIQ, and TheMarkets.com. ...
Cached Biology Technology:A new model for studying Parkinson's 2Tuning graphene film so it sheds water 2Research 2.0 Initiates Coverage of Viral Genetics and Publishes Research Report 2Research 2.0 Initiates Coverage of Viral Genetics and Publishes Research Report 3
(Date:10/29/2014)... is open for the Society of Interventional ... 2015 is the one place where interventional ... health care professionals can come together to ... technologies being presented, discussed and tested. ... invasive, image-guided medicine, SIR 2015 will feature ...
(Date:10/29/2014)... to make their food, but they also need protection from ... a group of molecules in plants that shields them from ... of the American Chemical Society , one team reports on ... Timothy Zwier and colleagues at Purdue University note that the ... serious damage to plant DNA and, as a result, hinder ...
(Date:10/29/2014)... all the rage. Thousands of scientists worldwide are conducting ... titanium dioxide nanoparticles from sun creams can get through ... from electronic products are as hazardous for the lungs ... food can get into the blood via the intestinal ... are flowing – and the number of scientific projects ...
Breaking Biology News(10 mins):SIR 2015: Interventional radiology shapes tomorrow's medicine 2Nanosafety research: The quest for the gold standard 2Nanosafety research: The quest for the gold standard 3
... in the most exciting and unsettling period in the ... in public. On the one hand, the ... the psychiatric diagnostic manual, DSM-V, representing the current best ... categorize the enormously complex pattern of human emotional, cognitive, ...
... University of Adelaide has shown for the first time ... greatly increasing the risk of complications for themselves and their unborn ... world, researchers from the University,s Robinson Institute compared data ... results have been published online ahead of print in the ...
... time that the blood vessels that transport blood to and ... just bodily pipelines. Arterioles and capillaries, the small vessels, actually ... blood they,re carrying. Biomedical engineers at Drexel University, who study ... how they do it. The team, which includes Drs. ...
Cached Biology News:What is the brain telling us about the diagnoses of schizophrenia and bipolar disorder? 2Smoking + asthma + pregnant = a dangerous combination 2Drexel researchers looking inside vessels to understand blood's ebb and flow 2
capacity 250 mL...
... to MSY2/YBOX2 ( Abpromise for ... Antigen: Synthetic peptide derived from ... of human YBX2. (Note: the ... Entrez ...
... CLS number is a ... to easily match Cornings ... no availability yet, please ... Sigma-Aldrich number (Z35,347-7) or ...
Standard Fluka For microscopy (Bact., Hist.)...
Biology Products: