Navigation Links
Efficient DNA transfection of primary CNS neurons using TransMessenger ,,, Transfection Reagent

after transfection of a GFP-encoding plasmid; C: fluorescence image of live cells 24 hours after transfection (same visual field as B). Cells were transfected in a 96-well format using 0.3 g of DNA and 2 l of TransMessenger Reagent per well.

To distinguish the cell types in the CryoCell cultures, cell-specific markers were detected using monoclonal primary antibodies (anti-MAP2 antibodies for neurons, anti-GFAP antibodies for astrocytes, the major subtype of glial cells in the brain). "Excellent Morphology of CryoCell Cultures" shows a typical result. Staining with anti-MAP2 antibodies reveals a well-developed neurite network and brightly stained somata, indicating good morphology and differentiation of the neurons. Staining with anti-GFAP antibodies shows glial-cell astrocytes with a stellate morphology. Serum-free cultures contain many fewer astrocytes than serum-containing cultures (data not shown). Transfected and untransfected cultures display the same GFAP- and MAP2-staining pattern. The immunofluorescence results show that primary cultures of cortical neurons generated from CryoCells have morphologies and characteristics comparable to cultures derived from freshly prepared cells.

Excellent Morphology of CryoCell Cultures Cells stained with anti-MAP2 primary antibodies and Cy3-labeled secondary antibodies, 24 hours after transfection: A: Untransfected cells; B: transfected cells, anti-MAP2 staining; C: transfected cells, GFP fluorescence; D: superimposed images from B and C. Cells stained with anti-GFAP primary antibodies and Cy3-labeled secondary antibodies: E: Untransfected cells; F: transfected cells, anti-GFAP s
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
9. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
10. Efficient and Reliable Linear Amplification of cRNA
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
Post Your Comments:
(Date:9/30/2014)... D.C., September 30, 2014 Applying a well-known ... researchers at Harvard University has demonstrated the ability ... -- work that holds promise for making future, ... detailing the sides of next-gen rocket ships and ... light passes through oil in water, it becomes ...
(Date:9/30/2014)... NEW YORK , Sept. 30, 2014 /PRNewswire/ ... trends and segments with highest potential revenues Do ... Visiongain,s updated report gives you revenue predictions for ... ahead. For those therapies you find financial data, ... new analysis you get sales forecasts to 2024 ...
(Date:9/30/2014)... Proove Biosciences , the commercial and ... a new genetic test built upon research from their ... will provide physicians with an objective way to ... are stratified between individuals. With this new test, physicians ... a genetic predisposition that will affect their pain tolerance. ...
(Date:9/30/2014)... 2014 Fast, semi-automatic creation ... in documents ChemAxon , a ... services for life science research, launches ChemCurator, an ... structures from documents. Markush structures are ... generating and understanding Markush structures is a difficult ...
Breaking Biology Technology:Taking thin films to the extreme 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 2Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 3ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2
... Auxogyn, Inc., a privately held medical technology company ... acquired an exclusive license from Stanford University to develop ... in the field of assisted reproduction to significantly improve ... procedures. The technology licensed from Stanford is ...
... Calif., Oct. 4 Dainippon Sumitomo Pharma Co., Ltd. ... have entered into an option agreement to co-develop a ... the agreement, DSP has received an option for exclusive ... a promising cell therapy drug candidate being developed by ...
... BOTHELL, WA and VANCOUVER, Oct. 4 /PRNewswire-FirstCall/ - OncoGenex Pharmaceuticals, ... of OncoGenex will present at the 9th Annual BIO Investor ... The Palace Hotel in San Francisco, CA. Live ... Relations page of the OncoGenex Web site at http://ir.oncogenex.com ...
Cached Biology Technology:Auxogyn Licenses Non-invasive Embryo Assessment Technology From Stanford University 2Dainippon Sumitomo Pharma and SanBio Collaborate on Cell Therapy for Stroke Recovery 2OncoGenex Pharmaceuticals to Present at the 9th Annual BIO Investor Forum 2
(Date:9/30/2014)... news release is available in German . ... in their behavioural response to risky situations such as ... for Ornithology now found in a long-term study on ... with both metabolic rate and ambient temperature. High metabolic ... behaviour, as in these scenarios birds were more likely ...
(Date:9/30/2014)... threats in today,s ocean. From overfishing and pollution ... ecosystems are disappearing at unprecedented rates around the ... surrounding the island of Moorea in French Polynesia ... coral guard-crabs. New research from the National Museum ... McKeon and the museum,s predoctoral fellow Jenna Moore ...
(Date:9/30/2014)... 2014 Winners of The Economist,s prestigious Innovation ... to take place at the JW Marriott Hong Kong on Friday, ... will share their experiences and the lessons those hold for others. ... innovation over the past decade, will be presented at a ceremony ... summit. It will be the first time the ceremony has come ...
Breaking Biology News(10 mins):Risky metabolism 2Smithsonian scientists discover coral's best defender against an army of sea stars 2Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 2Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 3Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 4Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 5Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 6Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 7
... , A scientist from Nanyang Technological University (NTU) may be ... a little sea creature called the mantis shrimp. The ... preferred material for medical implants and military body armour ... Science , the world,s top scientific journal, and focused ...
... high-resolution image of a palm tree in Brazil, which ... on nectars and pollens, was the top jury selection ... GigaBlitz. It,s also an example of what organizers hope ... The Nearby Nature GigaBlitz events are citizen science ...
... coastal restoration, The Nature Conservancy and a team of scientists ... led by the University of Cambridge conducted an in-depth study ... actual biomass (the "living weight") of oyster reefs in dozens ... ecology with real numbers, published today in Proceedings of ...
Cached Biology News:NTU researchers study little mighty creature for scientific breakthrough 2NTU researchers study little mighty creature for scientific breakthrough 3Citizen scientists to document biodiversity with high-resolution imagery during summer solstice 2New report puts real numbers behind history of oyster reefs 2
... Asb-2 (Ankyrin repeat and SOCS box-containing protein) ... peptides containing amino acids 75-93 and 348-368 ... (Imgenex Catalog No. 40149) can be used ... react with mouse and rat Asb-2, based ...
... Rabbit polyclonal to ErbB 2 ... Abpromise for all tested applications). ... peptide: AENPEYLGLDVPV conjugated to KLH, corresponding ... of Human c-ErbB2 ...
SHP-2 Antibody...
Sheep polyclonal to ErbB 2 ( Abpromise for all tested applications). Antigen: Recombinant full length c-ErbB2 protein (Human). Entrez GeneID: 2064 Swiss Protein ID: P04626...
Biology Products: