Navigation Links
Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini

Recombinant Enterokinase for highly specific digestion of purified proteins or proteins in crude extracts

Ed Marsh * Johanna Claycomb * ChaoFeng Zheng * Denise Wyborski * John C. Bauer * Joseph A. Sorge * A.K. Bernardino * Peter Vaillancourt
Stratagene Cloning Systems, Inc.

Stratagene introduces recombinant Enterokinase, an efficient, sitespecific protease that cleaves at the carboxy terminus of its recognition target. Cleavage of fusion proteins that contain the enterokinase (EK) recognition sequence positioned between an Nterminal purification tag and the Cterminal polypeptide of interest results in Cterminal fusion partners that have no extraneous amino acids derived from the cleavage site. Thus, recombinant proteins with native amino acid sequence are produced. Stratagene's purified Enterokinase is free of contaminating proteases, functions efficiently over a wide range of reaction conditions and is provided with Soybean Trypsin Inhibitor Agarose (STI Agarose) for rapid and quantitative removal of EK from digests.

figure 1

Site-specific proteases and their cognate target amino acid sequences are often used to remove the fusion protein tags that facilitate enhanced expression, solubility, secretion or purification of the fusion protein. The serine protease enterokinase (EK) is particularly attractive because it cleaves after the C-terminal amino acid of its recognition site, (aspartic acid)4lysine. This cleavage produces fusion proteins that have native amino termini following removal of N-terminal tags by cleaving with EK (figure 1). In the past, the use of EK for fusion protein cleavage was hampered by the generally poor quality of the commercially a
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
3. Efficient Transfection of Neurospora Crassa
4. Efficient Transfection of Neurospora Crassa
5. Efficient Recovery of Ultrapure Plasmid DNA
6. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
7. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
8. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
9. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
10. Efficient and Reliable Linear Amplification of cRNA
11. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Efficient Cleavage Fusion Proteins Yield Native Amino Termini

(Date:9/2/2015)... Sept. 2, 2015 Axovant Sciences Ltd. (NYSE:  AXON), ... dementia, today announced upcoming presentations at three investor conferences: ... Baird 2015 Healthcare Conference in New York ... September 10 at 2:00 PM at the BioCentury NewsMakers Conference ... Broadway Hotel & Conference Center , Thursday, September 17 ...
(Date:9/2/2015)... , Sept. 2, 2015 ... announced the addition of Jain PharmaBiotech,s new ... to their offering. ... pharmaceutical and biotechnology industries is anticipated. Nanotechnology ... drug development - from formulations for optimal ...
(Date:9/2/2015)... ... September 02, 2015 , ... ... systems, analytical testing, education and consulting services for the medical device sector, ... products/processes development, design control, validation, quality assurance and regulatory compliance. Avarent will ...
(Date:9/2/2015)... Research and Markets ... Jain PharmaBiotech,s new report "DNA Sequencing - ... This report briefly reviews basics of ... their applications. Current large and small sequencers are ... applications of sequencing are described including those for ...
Breaking Biology Technology:Axovant Sciences To Present At Upcoming Investor Conferences 2Axovant Sciences To Present At Upcoming Investor Conferences 3Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 2Global Nanobiotechnology Applications, Markets and Companies Report 2015-2024 3NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 2NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 3NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 4NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 5NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 6NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 7Global DNA Sequencing Technologies Report 2015: Profiles of 130 Companies Involved in Sequencing and their 140 Collaborations 2
... part of a new strategic plan featuring enhancements to ... has announced the launch of nonstop Midwest Connect ... serves Research Triangle Park , beginning June 1. ... hostile takeover bid by AirTran Holdings, noted that Raleigh/Durham ...
... NimbleGen Systems announced on Friday afternoon that it has ... and number of shares are yet to be determined. J.P. ... Partners, Leerink-Swann & Company and Robert W. Baird & Co. ... $12.5 million venture capital investment at the close of 2006 ...
... comes to health information technology, Greg Smith doesn't have to ... is thinking. , ,Smith, senior vice president and CIO ... the business side of the Wheaton shop. Armed with a ... resource business, and now sits on Hewlett Packard's ...
Cached Biology Technology:CIO Leadership Series: Greg Smith, Wheaton Franciscan Healthcare 2CIO Leadership Series: Greg Smith, Wheaton Franciscan Healthcare 3CIO Leadership Series: Greg Smith, Wheaton Franciscan Healthcare 4
(Date:8/28/2015)... 2015 According to a new ... Assessment Type (Pen & Paper Based, Hosted, Biometrics), Service, ... Academic Research), Vertical and Region - Global Forecast to ... to grow from USD 2.4 Billion in 2015 to ... Growth Rate (CAGR) of 25.6% from 2015 to 2020. ...
(Date:8/20/2015)... 20, 2015 The wearable technology market ... active and healthy. However, wearable technology has the ... to help improve diagnostic capabilities and therapeutic outcomes. ... Wearable Technologies Conference 2015 , where ... wearables in healthcare.    "Over the ...
(Date:8/18/2015)... , Aug. 17, 2015 Research ... addition of the "Global Biometric Authentication & ... - Estimation & Forecast, 2015-2020" report to ... authentication and identification systems market is expected to ... 2015 to 2020 and generate over $25 billion ...
Breaking Biology News(10 mins):Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 2Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 3Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 4Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 2Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 3Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2
... presumed that the last glacial period denuded the Scandinavian ... weather began and melted away the ice cover some ... research headed by Professor Eske Willerslev from the Centre ... the University of Uppsala, Sweden, and Inger Greve Alsos ...
... potential genetic regulators in planarian stem cells, Whitehead Institute ... two main functions of stem cells. Three of the ... similar to those known to regulate mammalian embryonic stem ... attractive model for studying stem cell biology in vivo. ...
... Cut marks found on Ice Age bones indicate that humans ... known. Dr. Brian Redmond, curator of archaeology at The Cleveland ... in the Feb. 22, 2012 online issue of the journal ... animal bones found in 1998 in the collections of the ...
Cached Biology News:Sturdy Scandinavian conifers survived Ice Age 2Sturdy Scandinavian conifers survived Ice Age 3Planarian genes that control stem cell biology identified 2Planarian genes that control stem cell biology identified 3Research reveals first evidence of hunting by prehistoric Ohioans 2
GOAT ANTI HUMAN PARAINFLUENZA VIRUS 2+3...
... Cary 50 Microplate Reader accessory ... per analysis by extending the ... award-winning Cary 50 instruments. Use ... 384 samples in microlitre volumes, ...
IMAGEQUANT 300 WKSTN, 1 EA. Category: ImageQuant CCD Imagers....
PAb to Hu-IFN-Alpha/Beta R2 [(IFNAR2) CD118], Rabbit Serum, neutralizing Binds to but does not neutralize human interferon alpha/beta receptor...
Biology Products: