Navigation Links
Efficient Cellular Fractionation for RNA ,,, and Protein Isolation

Efficient Cellular Fractionation for RNA and Protein Isolation


Ambion's Protein and RNA Isolation System, PARIS, uses a rapid and simple procedure to isolate both total RNA and native protein from the same experimental sample. Cultured cells and tissues that are either fresh, frozen, or treated with RNA stabilization reagents such as RNAlater and RNAlater-ICE, are all compatible with the procedure. This kit provides a unique tool for researchers who need both RNA and protein for various downstream analysis, reducing time, cost, and variability between independent experimental samples. The PARIS Kit also permits separation of nuclear and cytoplasmic fractions from fresh cultured cells prior to RNA and/or protein isolation. Here, we tested the procedure with various mammalian cell types and show that both RNA and native protein are efficiently recovered from nuclear and cytoplasmic fractions with virtually no cross contamination between the two cellular compartments.


Fast and Simple Cellular Fractionation
To obtain total cellular protein and RNA using the PARIS Kit, whole cells are first homogenized in ice-cold Cell Disruption Buffer to prepare a total cell lysate. Since the homogenization is performed quickly on ice and in the presence of detergent, both protein and high quality RNA can be isolated from this lysate. For RNA isolation, a part of the tota
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
9. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
10. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
11. Efficient and Reliable Linear Amplification of cRNA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Efficient Cellular Fractionation for RNA and Protein Isolation

(Date:6/30/2015)... CALGARY , June 30, 2015 /PRNewswire/ - ... ("Shareholders") of common shares of Resverlogix have approved ... to Eastern Capital Limited of 5,600,000 units at ... Private Placement"), each unit being comprised of one ... for aggregate proceeds of approximately CAD$15 million; and ...
(Date:6/30/2015)... 2015  NASA astronaut Kjell Lindgren , who is ... first mission to the International Space Station , will ... a.m. EDT Tuesday, July 7. Lindgren will participate ... Russia . The interviews will be preceded at ... mission training. To schedule an interview, media must ...
(Date:6/30/2015)... June 30, 2015  Today, Americord Registry, one of the ... Andrew Horne , a partner at Kirkland & Ellis LLP, ... Logo - http://photos.prnewswire.com/prnh/20150630/226958LOGO   "We are ... Board," says Martin Smithmyer , CEO of Americord. "He ... career that will assist Americord,s growth and vision." ...
(Date:6/30/2015)... Kent, WA (PRWEB) , ... June 30, 2015 ... ... offers a hydrophilic filter that does not sacrifice selectivity to achieve faster flow ... construction combines extremely fine pores with significant open space to allow easy liquid ...
Breaking Biology Technology:Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 2Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 3NASA Astronaut Preps for First Space Station Mission, Available for Media Interviews 2Americord Registry Announces Andrew Horne as Advisory Board Member 2New Nanofiber Membrane Provides Fast, Efficient Filtration 2
... Alimentary Health Ltd.,(Alimentary Health), the development stage specialty ... use of probiotics in the,treatment of Irritable Bowel ... Alimentary Health probiotic technology marketed as,Bifantis(R), is the ... in IBS symptoms. Alimentary Health is a ...
... Bioponic,Phytoceuticals, Inc. (OTC: BPYT) ("Bioponic"), an ... serving the Complementary,Alternative Medicine (CAM) and nutraceutical ... Sales and Marketing of Southern California as ... including the states,of California, Washington, Nevada, Arizona, ...
... Investor Conference, November 8, Features ... Mid-cap Public Growth Companies Renowned Investment Banker and ... Stelios Papadopoulos, PhD Provides ... Biotechnology,Council,s ninth annual MASS Opportunities Investor Conference, New,England,s largest ...
Cached Biology Technology:Recent Study Confirms Alimentary Health's Bifantis(R) Demonstrates Efficacy in the Treatment of Irritable Bowel Syndrome 2Recent Study Confirms Alimentary Health's Bifantis(R) Demonstrates Efficacy in the Treatment of Irritable Bowel Syndrome 3Recent Study Confirms Alimentary Health's Bifantis(R) Demonstrates Efficacy in the Treatment of Irritable Bowel Syndrome 4Bioponic Phytoceuticals to Roll Out Multiple Products to 1,000 Major Health Food Stores in the Western United States 22007 Massachusetts Biotechnology Council MASS Opportunities Investment Conference Showcases Leading Growth Companies 22007 Massachusetts Biotechnology Council MASS Opportunities Investment Conference Showcases Leading Growth Companies 3
(Date:6/15/2015)... Jun. 15, 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... the growing mobile commerce market, announces that its leading payment ... media at Digital Experience! @ NYC June 18th. ... the latest innovation in technology to over 300 of the ... at the Metropolitan Pavilion in New York City ...
(Date:6/11/2015)... Va. , June 11, 2015 ... technology, announced today that its IdentityX Mobile Authentication ... (Fast IDentity Online) Alliance is an industry consortium ... open standards for simpler, stronger authentication.  In order ... must pass a rigorous series of tests that ...
(Date:6/9/2015)... 2015   MedNet Solutions , an innovative SaaS-based ... clinical research, will be providing demonstrations of its ... 2015 Drug Information Association (DIA) Annual Meeting in ... presentations will include previews of many exciting new enhancements ... iMedNet 2015 Feature 1 Release. ...
Breaking Biology News(10 mins):Wocket Smart Wallet Exhibiting at Pepcom Digital Experience! @ NYC June 18th 2Wocket Smart Wallet Exhibiting at Pepcom Digital Experience! @ NYC June 18th 3Daon's IdentityX Mobile Authentication Platform One of First Products to be Certified by FIDO Alliance 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 3
... York, NY2 July 2013Dr. Carissa Klein of Australia was ... Education (ASPIRE), sponsored by John Wiley & Sons, Inc., ... Indonesian Ministry of Research and Technology. Dr. ... the University of Queensland that uniquely addresses the issue ...
... by which an atom donates an electron to another ... and is of intense research because of the implications ... interact, electron transfer takes place in a few quadrillionths ... studying this event requires very time-sensitive techniques like ultrafast ...
... for many things clean water, land for crops or ... has been challenging to figure out how to sustain the ... in any given landscape because an improvement in one ... ecologists at the University of WisconsinMadison report this week (July ...
Cached Biology News:ASPIRE prize winner balances ocean conservation and socioeconomic viability 2Solving electron transfer 2Mapping the benefits of our ecosystems 2Mapping the benefits of our ecosystems 3
The iEMS Incubator/Shaker is a high performance microplate incubator and orbital shaker. Its superior temperature control and efficient orbital shaking reduce incubation times. It can incubate up to ...
iEMS Thermal Microplate Holder...
... Orbital Shakers have the durability ... stacked 3-high, and the uniformity ... "soft shake" applications. Heavily ... systems, our stackers are designed ...
... The VARIOKLAV 75 S and 135 S steam ... double bottoms combine the advantages of a double-walled ... use, effective deaeration and drying by built-in jacket ... - fast cooling. The chamber is free of ...
Biology Products: