Navigation Links
Efficient Cellular Fractionation for RNA ,,, and Protein Isolation

Efficient Cellular Fractionation for RNA and Protein Isolation


Ambion's Protein and RNA Isolation System, PARIS, uses a rapid and simple procedure to isolate both total RNA and native protein from the same experimental sample. Cultured cells and tissues that are either fresh, frozen, or treated with RNA stabilization reagents such as RNAlater and RNAlater-ICE, are all compatible with the procedure. This kit provides a unique tool for researchers who need both RNA and protein for various downstream analysis, reducing time, cost, and variability between independent experimental samples. The PARIS Kit also permits separation of nuclear and cytoplasmic fractions from fresh cultured cells prior to RNA and/or protein isolation. Here, we tested the procedure with various mammalian cell types and show that both RNA and native protein are efficiently recovered from nuclear and cytoplasmic fractions with virtually no cross contamination between the two cellular compartments.


Fast and Simple Cellular Fractionation
To obtain total cellular protein and RNA using the PARIS Kit, whole cells are first homogenized in ice-cold Cell Disruption Buffer to prepare a total cell lysate. Since the homogenization is performed quickly on ice and in the presence of detergent, both protein and high quality RNA can be isolated from this lysate. For RNA isolation, a part of the tota
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
4. Efficient Transfection of Neurospora Crassa
5. Efficient Transfection of Neurospora Crassa
6. Efficient Recovery of Ultrapure Plasmid DNA
7. Efficient and Reliable PCR Setup Using Eppendorf MasterMix
8. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
9. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
10. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
11. Efficient and Reliable Linear Amplification of cRNA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Efficient Cellular Fractionation for RNA and Protein Isolation

(Date:7/11/2014)... , July 11, 2014 EPFL ... announce the appointment of John P. Donoghue . ...   in  Geneva . The new ... Biotech in Geneva , has named ... neuroscientists. John P. Donoghue , founder of Brown ...
(Date:7/10/2014)... 10, 2014 Product and process ... low abundance and are often “lost in the ... , Join presenters Dr. Rowel Tobias, Senior Scientist, ... Anders, Head of Quality at Nanotherapeutics, Inc., to ... can speed detection and quantitation while achieving the ...
(Date:7/10/2014)... Poway, CA (PRWEB) July 10, 2014 Robert ... Regenerative Veterinary Medicine company, is proud to announce the relaunch ... Pets, launching with a new series called “ What are ... to give an honest and straightforward foundation in the basics ... an educated decision on the right type of treatment when ...
(Date:7/10/2014)... , July 10, 2014 ... Dmitry Medvedev , presented Russia,s ... BIOCAD . The Company is developing a unique ... MabNext project BIOCAD develops a number of innovative drugs ... autoimmune diseases. The ceremony took place at the International ...
Breaking Biology Technology:John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 2John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 3Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2
... one of six states to receive a $50,000 grant ... network further into the Internet age. , ,Wisconsin's grant ... of Justice Assistance, which houses the Wisconsin Justice ... maintaining, promoting and coordinating automated justice information systems. , ...
... the learning acquired in the program but rather the blue-chip roster ... mythology goes, you can call on a fellow alum to help ... will be of value once again. , ,Though I can't say ... can attest to the power of alumni connections. , ,While I'm ...
... of health-care and business technology, announced a seven-year contract with ... Information System (MMIS). The new system is expected to be ... contracts EDS to implement its InterChange health system on the ... Services . The contract is worth approximately $189 million and ...
Cached Biology Technology:State receives grant for justice information sharing 2Pay attention to your alumni connections when job hunting 2Pay attention to your alumni connections when job hunting 3Pay attention to your alumni connections when job hunting 4State picks long-time vendor for $189m Medicaid information system 2
(Date:7/11/2014)... Rio de Janeiro, Brazil- In the brains of ... mechanism that allows an electric or chemical signal ... another. Chemical synapses, which are the most abundant ... inhibitory. Synapse formation is crucial for learning, memory, ... and inhibitory synapses critical for brain function. ...
(Date:7/10/2014)... , July 1, 2014 Smart ... way for convenience and improved security: NXT-ID, Inc. (OTCBB: NXTD), ... GOOG ), Apple Inc. (NASDAQ: AAPL ... Corporation (NYSE: MA) NXT-ID, Inc., (OTCQB: NXTD) a biometric ... to announce that the second series of 30 second spots ...
(Date:7/10/2014)... -- Research and Markets ( http://www.researchandmarkets.com/research/h7h8lq/gesture ) has ... Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, ... to their offering http://photos.prnewswire.com/prnh/20130307/600769 ... Sensing Market to Grow Exponentially. Touch-less sensing applications ... companies were unable to leverage this technology due ...
Breaking Biology News(10 mins):Blame it on the astrocytes 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 4Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 5Global Gesture Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, Capacitive) Market - Forecasts to 2020 2
... the Texas Biomedical Research Institute may help explain why ... at-risk for atypical fractures in the long bones in ... Ph.D. and colleagues at the Southwest Research Institute and ... differences in the microstructure of their femurs that she ...
... Antibiotic resistance is an international reality whose solution ... an infectious disease physician. "The big problem is ... not only can they lead to side effects like ... is development of multidrug-resistant organisms," said Dr. Josѐ A. ...
... the genetic signature of respiratory syncytial virus (RSV), the leading ... a key step toward a better understanding of the immune ... vaccine and a tool that could allow physicians to determine ... study, from a team at The Research Institute at Nationwide ...
Cached Biology News:New studies may explain fractures in some who take osteoporosis drugs 2Antibiotic resistance is a international issue that better education can address 2Genetic signature identified for RSV, the leading cause of infant hospitalizations worldwide 2Genetic signature identified for RSV, the leading cause of infant hospitalizations worldwide 3
... DNA Kit enables efficient extraction of DNA fragments from ... agarose gels. The procedure uses GelMelt solution to dissolve ... to a silica membrane in a spin column format. ... in low-salt buffer. Each spin column can bind up ...
... efficient extraction of DNA from agarose gels The ... fragments of 150 bp to > 12,000 bp in ... to dissolve the gel slice, followed by adsorption of ... column format. After a wash step, the purified DNA ...
When added to either StemSep CD4+ or CD8+ T Cell Enrichment cocktails, human HLA-DR TAC is designed to deplete human HLA-DR from samples to isolate resting CD4+ or CD8+ T cells....
BlotGel application pool -see Blotters (Semi-Dry) for more info...
Biology Products: