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Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results

hot top disabled (data not shown). These results correlate well with the temperature profile obtained from the PTC-100, which showed a difference of up to 1.1 C from the middle to the edges of the block. These PCR results corroborate the block temperature profiles and indicate that researchers can obtain more consistent PCR results from well to well using the RoboCycler 96 thermal cycler.

Conclusions

The RoboCycler temperature cycler provides more consistent fluid temperature profiles between center and edge well positions during PCR thermal cycling than the MJ Research PTC-100. When comparing PCR results from both cyclers, the RoboCycler 96 also shows a greater well-to-well uniformity of amplified products. This temperature uniformity results from the RoboCycler systems unique four-block design that uses thermal blocks and heating techniques optimized for block temperature uniformity.

REFERENCES

  1. Cayouette, M. et. al. (1997) Strategies 10: 75-76.

* US Patent number 5,525,300 and patents pending


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