( ere incubated with the complexes for 4....)Effectene Transfection Reagent provides efficient gene delivery ,,, to primary neuronal cell cultures

ere incubated with the complexes for 4.5 hours, the cells were removed, and incubated for a further 24 hours. A Cells were fixed and stained for MAP2-immunoreactivity which specifically labels the somatodendritic compartments of neurons (red). B Cell nuclei were stained using DAPI (blue). C Cells expressing EGFP appear green. D Superimposed images.

Conclusions

• Effectene Transfection Reagent can be used for efficient gene delivery into differentiated neurons in primary cell culture.
  
• Small amounts of DNA together with Effectene Transfection Reagent delivered high transfection efficiencies and expression levels, without damaging neurons.
  
• Cell death in 24-well plates was negligible if less than 10 l Effectene Reagent was used per well and the exposure of cells to transfection complexes was less than 5 hours.
  
• Effectene Transfection Reagent is a powerful tool for in vitro delivery of genes to nondividing cells - particularly differentiated neurons - and therefore greatly facilitates studies of neurophysiology in living cells.

References

1. Meier, J., Vannier, C., Serg, A. Triller, A., and Choquet, D. (2001) Fast and reversible trapping of surface glycine receptors by gephyrin. Nat. Neurosci. 4, 253.

2. Meier, J., Meunier- Durmort, C., Forest, C., Triller, A., and Vannier, C. (2000) Formation of glycine receptor clusters and their accumulation at synapses. J. Cell Sci. 113, 2783.




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