( e of cell types (see figures High ...)Effectene Transfection Reagent

e of cell types (see figures "High Transfection Efficiencies Using Effectene Reagent" and "Transfection of Oligonucleotides Using Effectene Reagent"). Because transfection can be performed in the presence of serum and requires low amounts of DNA (see figure "Serum and DNA Quantity vs. Transfection Efficiency"), cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells (see figure "Effectene Reagent with Primary Cells"). A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site www.qiagen.com/transfectiontools/.

Serum and DNA Quantity vs. Transfection Efficiency



Influence of serum and DNA quantity on transfection using Effectene Reagent. 2 x 104 COS-7 cells were seeded per well in 96-well plates one day before transfection. Cells were transfected using 0.011.0 g of a beta-galactosidasereporter plasmid and 0.088.0 l Enhancer (DNA: Enhancer ratio of 1:8) and 2 l Effectene Reagent, in either the presence or absence of serum. Each bar represents the average efficiency from four replicates 48 h post-transfection.

Effectene Reagent with Primary Cells

Expression of green fluorescent protein (GFP) in primary rabbit aortic smooth muscle cell
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