Navigation Links
Effectene Transfection Reagent

For transfection of a wide variety of cells, particularly effective for primary cells

Features and benefits
  • Fast and easy transfection
  • No transfection-complex removal needed for most cell lines
  • Transfection in the presence of serum
  • Ideal for primary cells or sensitive cell lines
  • High transfection efficiencies and minimal cytotoxicity
  • Low DNA requirement

Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids (see figure "Model of Effectene Principle"), providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods (1, 2).

Model of Effectene Principle

High Transfection Efficiencies Using Effectene Reagent
Comparison of transfection efficiencies using Effectene Reagent and two commonly used lipid-based reagents. Murine teratocarcinoma F9 cells (5 x 105) were transfected in 6-well plates with a luciferase-reporter plasmid using optimized conditions based on the manufacturer's instructions for each reagent. Transfection efficiencies were determined by measuring luciferase activity 48 h post-transfection, and are given as relative light units (RLU). (Data kindly provided by I. Clavereau, D. Petitprez, and I. Van Seuningen, Unit INSERM 377, Place de Verdun, Lille Cedex, France.)

Transfection of Oligonucleotides Using Effectene Reagent

C6 glioblastoma cells (1 x 104) were transfected with either naked FITC-labeled CD44 antisense oligodeoxynucleotide (ODN) or with EffecteneODN complexes. Intracellular accumulation and distribution of the transfected ODNs was analyzed 16 h post-transfection by immunofluoresence. A Naked ODNs (2 g). B ODNs (0.04 g) complexed with Effectene Reagent. Transfected ODNs are green in the photos. (Data kindly provided by G. Beutel and M. Schott, Institute of Neuropathology, Hanover Medical School, Hanover, Germany.)


The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 25 minutes. Effectene Reagent is then added and the mixture is incubated for 510 minutes to allow EffecteneDNA complexes to form. The complexes are mixed with growth medium (which can contain serum and antibiotics), and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression.


Effectene Reagent is suitable for transient and stable transfection of a broad rang e of cell types (see figures "High Transfection Efficiencies Using Effectene Reagent" and "Transfection of Oligonucleotides Using Effectene Reagent"). Because transfection can be performed in the presence of serum and requires low amounts of DNA (see figure "Serum and DNA Quantity vs. Transfection Efficiency"), cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells (see figure "Effectene Reagent with Primary Cells"). A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site

Serum and DNA Quantity vs. Transfection Efficiency

Influence of serum and DNA quantity on transfection using Effectene Reagent. 2 x 104 COS-7 cells were seeded per well in 96-well plates one day before transfection. Cells were transfected using 0.011.0 g of a beta-galactosidasereporter plasmid and 0.088.0 l Enhancer (DNA: Enhancer ratio of 1:8) and 2 l Effectene Reagent, in either the presence or absence of serum. Each bar represents the average efficiency from four replicates 48 h post-transfection.

Effectene Reagent with Primary Cells

Expression of green fluorescent protein (GFP) in primary rabbit aortic smooth muscle cell s transfected using Effectene Reagent. 1 x 105 cells were seeded one day prior to transfection, and transfections were performed in 6-well plates using 0.4 g of a GFP-reporter plasmid and 10 l Effectene Reagent per well. Cells were viewed 24 h post-transfection by fluorescence microscopy. Approximately 40% of the cells were transfected, as determined by FACS analysis. (Data kindly provided by K. Veit, 2nd Medical Clinic, Dept. Clinical Pharmacology, Mainz, Germany.)

High-throughput transfection

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.

Cited References

1. Ausubel, F.M., et al. eds. (1991) Current Protocols in Molecular Biology, Wiley Interscience, New York.
2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. (1989) Molecular cloning a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.



Page: All 1 2 3 4

Related biology technology :

1. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
2. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
3. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
4. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
5. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
6. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
7. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
8. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
9. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
10. Efficient Transfection of Neurospora Crassa
11. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
Post Your Comments:

(Date:10/13/2015)... Oct. 13, 2015 Applied BioMath ( ... decision points throughout the drug invention process, announced that ... 2015 on October 20-22, 2015 in Boston ... Co-Founder, President, and CEO of Applied BioMath, will present ... research to clinical trials" on Wednesday October 21st 2015 ...
(Date:10/13/2015)... October 13, 2015      Q ... company, has entered into a strategic relationship with Wombat ... and Paris, France based strategic ... --> This collaborative arrangement gives Q BioMed ... advisory team as well as long established pharmaceutical industry ...
(Date:10/13/2015)... ... ... Clinovo recently appointed Jeff Parr and Ed Donaldson to its Business Development Management ... Jeff Parr has spent the past decade providing quality management software and consulting services ... Sciex to name a few. He is based in Salt Lake City, a ...
(Date:10/12/2015)... THOUSAND OAKS, Calif. and BRUSSELS ... and UCB (Euronext Brussels: UCB) today presented additional findings from ... trial. 1 The findings were presented today in an ... Mineral Research (ASBMR) 2015 Annual Meeting in Seattle ... 2 --> The small exploratory sub-study ...
Breaking Biology Technology:
(Date:9/30/2015)... YORK , Sept. 30, 2015  The global glucose ... billion for 2015. So says a new report on the ... diabetes management segment dominate the market, followed by continuous glucose ... publisher. Kalorama reports on the market for these products in its ... ...
(Date:9/28/2015)... Sept. 28, 2015 CLEAR, the ... its expedited traveler service is coming to ... travel, bringing a frictionless experience, serious speed ... "CLEAR offers our travelers an expedited ... said Jim Smith , Executive Director, ...
(Date:9/28/2015)... According to a new market ... (Scanner & Others), Application (Access Control & Others), Industry ... Geography Global - Forecast to 2020", published by MarketsandMarkets, the ... Million by 2020, at a CAGR of 23.40% between ... data T ables and 66 Figures ...
Breaking Biology News(10 mins):