( ...)Effectene Transfection Reagent

For transfection of a wide variety of cells, particularly effective for primary cells

Features and benefits

• Fast and easy transfection
  
• No transfection-complex removal needed for most cell lines
  
• Transfection in the presence of serum
  
• Ideal for primary cells or sensitive cell lines
  
• High transfection efficiencies and minimal cytotoxicity
• Low DNA requirement

Principle

Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids (see figure "Model of Effectene Principle"), providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods (1, 2).

Model of Effectene Principle

High Transfection Efficiencies Using Effectene Reagent
Comparison of transfection efficiencies using Effectene Reagent and two commonly used lipid-based reagents. Murine teratocarcinoma F9 cells (5 x 105) were transfected in 6-well plates with a luciferase-reporter plasmid using optimized conditions based on the manufacturer's instructions for each reagent. Transfection efficiencies
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