otal RNA separated on a denaturing gel will have sharp, clear 28S and 18S rRNA bands (eukaryotic samples). The 28S rRNA bands of the samples used with or without PLG are approximately twice as intense as the 18S rRNA band (Fig. 2). This 2:1 ratio (28S:18S) is a good indication that the RNA is completely intact.

Fig. 2:. Total RNA were separated by gel electrophoresis beside the Gene Ruler DNA Marker 1010,000 bp (Fermentas) on a 1% denaturing agarose gel.

Lane 1: Total RNA prepared with the use of Eppendorf Phase Lock Gel. Lane 2: Total RNA prepared without the use of Eppendorf Phase Lock Gel. Reverse Transcription PCR

In each preparation, an 844 bp fragment of the human p53 cDNA could be amplified from RNA prepared with or without the use of PLG. As shown in Fig. 3, the amplicons are of the same size and approximately the same intensity, and there are no additional bands visible.

Fig. 3: RT-PCR performed on total RNA isolated with TRIzol and Phase Lock Gel. '"/>

Source:


Page: All 1 2 3 4 5 6
Related biology technology :

1. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
2. Embryonic stem cells, mouse
3. Primary cells, rat heart endothel
4. Primary cells, rat heart muscle
5. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
6. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
7. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
8. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
9. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
10. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
11. measurement of green fluorescent proteins and a red fluorescent protein in live cells in the Analyst GT multimode reader