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Easy Cosmid DNA Purification with StrataPrep Kits

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The StrataPrep plasmid miniprep and midiprep kits both produce high yields of all sizes of DNA from 2.9-kb plasmids to 45-kb cosmids. Both have rapid protocols using a modified alkaline lysis method to yield quality sequence with automated or manual methods. The amount of purified DNA required determines which kit to use. StrataPrep purified cosmid DNA can serve as the source material for subclone library construction used in shotgun sequencing projects.4

REFERENCES
  1. Basehore, S. and Braman, J. (1998) Strategies 12: 5-7.
  2. Braman, J. and Basehore, S. (1997) Strategies 10: 84-86.
  3. Held, P. (1998) Pharmaceutical Laboratory, February issue.
  4. Wilson, R. K. and Mardis, E. (1997) In B. Birren, et al (Eds.), Genome Analysis, A Laboratory Manual. pp. 397-403, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

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